Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0236 and Psest_0237 are separated by 102 nucleotides Psest_0237 and Psest_0238 are separated by 129 nucleotides
Psest_0236: Psest_0236 - Predicted SAM-dependent methyltransferases, at 246,797 to 247,993
_0236
Psest_0237: Psest_0237 - intracellular protease, PfpI family, at 248,096 to 248,635
_0237
Psest_0238: Psest_0238 - Predicted signal transduction protein, at 248,765 to 249,631
_0238
Position (kb) 248
249 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 247.287 kb on - strand, within Psest_0236 at 247.305 kb on + strand, within Psest_0236 at 247.305 kb on + strand, within Psest_0236 at 247.306 kb on - strand, within Psest_0236 at 247.306 kb on - strand, within Psest_0236 at 247.306 kb on - strand, within Psest_0236 at 247.306 kb on - strand, within Psest_0236 at 247.306 kb on - strand, within Psest_0236 at 247.341 kb on + strand, within Psest_0236 at 247.341 kb on + strand, within Psest_0236 at 247.341 kb on + strand, within Psest_0236 at 247.341 kb on + strand, within Psest_0236 at 247.342 kb on - strand, within Psest_0236 at 247.342 kb on - strand, within Psest_0236 at 247.342 kb on - strand, within Psest_0236 at 247.377 kb on + strand, within Psest_0236 at 247.377 kb on + strand, within Psest_0236 at 247.377 kb on + strand, within Psest_0236 at 247.378 kb on - strand, within Psest_0236 at 247.483 kb on - strand, within Psest_0236 at 247.483 kb on - strand, within Psest_0236 at 247.532 kb on + strand, within Psest_0236 at 247.532 kb on + strand, within Psest_0236 at 247.556 kb on + strand, within Psest_0236 at 247.556 kb on + strand, within Psest_0236 at 247.557 kb on - strand, within Psest_0236 at 247.557 kb on - strand, within Psest_0236 at 247.674 kb on + strand, within Psest_0236 at 247.674 kb on + strand, within Psest_0236 at 247.924 kb on - strand at 247.924 kb on - strand at 247.924 kb on - strand at 248.026 kb on - strand at 248.419 kb on - strand, within Psest_0237 at 248.453 kb on + strand, within Psest_0237 at 248.454 kb on - strand, within Psest_0237 at 248.600 kb on + strand at 248.601 kb on - strand at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.196 kb on + strand, within Psest_0238 at 249.197 kb on - strand, within Psest_0238 at 249.260 kb on + strand, within Psest_0238 at 249.261 kb on - strand, within Psest_0238 at 249.261 kb on - strand, within Psest_0238 at 249.338 kb on + strand, within Psest_0238 at 249.338 kb on + strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.563 kb on + strand at 249.563 kb on + strand at 249.563 kb on + strand at 249.564 kb on - strand at 249.564 kb on - strand at 249.581 kb on + strand at 249.581 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 247,287 - Psest_0236 0.41 +1.4 247,305 + Psest_0236 0.42 -0.8 247,305 + Psest_0236 0.42 +0.1 247,306 - Psest_0236 0.43 -0.6 247,306 - Psest_0236 0.43 -0.7 247,306 - Psest_0236 0.43 -0.3 247,306 - Psest_0236 0.43 -0.7 247,306 - Psest_0236 0.43 -3.0 247,341 + Psest_0236 0.45 +0.6 247,341 + Psest_0236 0.45 +1.5 247,341 + Psest_0236 0.45 -0.3 247,341 + Psest_0236 0.45 +1.0 247,342 - Psest_0236 0.46 -2.5 247,342 - Psest_0236 0.46 +0.5 247,342 - Psest_0236 0.46 +1.3 247,377 + Psest_0236 0.48 -1.2 247,377 + Psest_0236 0.48 +1.4 247,377 + Psest_0236 0.48 -0.0 247,378 - Psest_0236 0.49 +2.1 247,483 - Psest_0236 0.57 -0.8 247,483 - Psest_0236 0.57 +1.2 247,532 + Psest_0236 0.61 -0.6 247,532 + Psest_0236 0.61 -0.8 247,556 + Psest_0236 0.63 -0.0 247,556 + Psest_0236 0.63 +1.3 247,557 - Psest_0236 0.63 +1.9 247,557 - Psest_0236 0.63 +1.2 247,674 + Psest_0236 0.73 +0.8 247,674 + Psest_0236 0.73 -1.6 247,924 - -0.4 247,924 - -3.0 247,924 - +0.8 248,026 - +4.0 248,419 - Psest_0237 0.60 -2.0 248,453 + Psest_0237 0.66 -0.7 248,454 - Psest_0237 0.66 -0.8 248,600 + +1.0 248,601 - -0.8 249,176 + Psest_0238 0.47 -1.7 249,176 + Psest_0238 0.47 -0.0 249,176 + Psest_0238 0.47 -1.9 249,176 + Psest_0238 0.47 -1.6 249,176 + Psest_0238 0.47 -0.7 249,176 + Psest_0238 0.47 -0.0 249,176 + Psest_0238 0.47 -0.4 249,176 + Psest_0238 0.47 -3.9 249,177 - Psest_0238 0.48 -2.9 249,177 - Psest_0238 0.48 +1.9 249,177 - Psest_0238 0.48 -0.9 249,177 - Psest_0238 0.48 +2.2 249,177 - Psest_0238 0.48 -2.5 249,177 - Psest_0238 0.48 +0.3 249,177 - Psest_0238 0.48 -0.0 249,177 - Psest_0238 0.48 -1.3 249,177 - Psest_0238 0.48 +2.0 249,177 - Psest_0238 0.48 -2.5 249,196 + Psest_0238 0.50 -3.4 249,197 - Psest_0238 0.50 -2.2 249,260 + Psest_0238 0.57 +2.0 249,261 - Psest_0238 0.57 +0.9 249,261 - Psest_0238 0.57 -0.6 249,338 + Psest_0238 0.66 +1.7 249,338 + Psest_0238 0.66 -3.1 249,339 - Psest_0238 0.66 -2.3 249,339 - Psest_0238 0.66 -2.9 249,339 - Psest_0238 0.66 -1.5 249,563 + +3.1 249,563 + -3.0 249,563 + -3.1 249,564 - +1.1 249,564 - -0.4 249,581 + +0.6 249,581 + -1.3
Or see this region's nucleotide sequence