Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0193 and Psest_0194 are separated by 178 nucleotides Psest_0194 and Psest_0195 are separated by 110 nucleotides Psest_0195 and Psest_0196 are separated by 390 nucleotides
Psest_0193: Psest_0193 - conserved hypothetical protein, at 189,910 to 190,341
_0193
Psest_0194: Psest_0194 - hypothetical protein, at 190,520 to 190,756
_0194
Psest_0195: Psest_0195 - Small integral membrane protein, at 190,867 to 191,037
_0195
Psest_0196: Psest_0196 - Uncharacterized membrane protein, required for spore maturation in B.subtilis., at 191,428 to 192,657
_0196
Position (kb) 190
191
192 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 189.974 kb on - strand, within Psest_0193 at 190.138 kb on + strand, within Psest_0193 at 190.474 kb on - strand at 190.505 kb on + strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.881 kb on + strand at 190.881 kb on + strand at 190.881 kb on + strand at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.974 kb on - strand, within Psest_0195 at 190.974 kb on - strand, within Psest_0195 at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.090 kb on - strand at 191.090 kb on - strand at 191.090 kb on - strand at 191.222 kb on + strand at 191.222 kb on + strand at 191.222 kb on + strand at 191.324 kb on - strand at 191.358 kb on - strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.529 kb on - strand at 191.529 kb on - strand at 191.529 kb on - strand at 191.865 kb on + strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.906 kb on - strand, within Psest_0196
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 189,974 - Psest_0193 0.15 -0.7 190,138 + Psest_0193 0.53 -1.3 190,474 - +0.2 190,505 + -0.3 190,506 - +1.2 190,506 - -1.6 190,506 - -1.8 190,506 - +0.4 190,506 - -0.1 190,506 - +1.9 190,506 - -0.4 190,661 + Psest_0194 0.59 -3.8 190,661 + Psest_0194 0.59 +3.0 190,661 + Psest_0194 0.59 +0.9 190,661 + Psest_0194 0.59 -0.1 190,661 + Psest_0194 0.59 +2.4 190,661 + Psest_0194 0.59 +1.9 190,661 + Psest_0194 0.59 +1.4 190,661 + Psest_0194 0.59 +0.7 190,661 + Psest_0194 0.59 +0.7 190,661 + Psest_0194 0.59 +2.9 190,661 + Psest_0194 0.59 -0.2 190,661 + Psest_0194 0.59 +1.5 190,662 - Psest_0194 0.60 +1.7 190,662 - Psest_0194 0.60 -1.2 190,662 - Psest_0194 0.60 +2.6 190,662 - Psest_0194 0.60 +3.5 190,662 - Psest_0194 0.60 +0.1 190,662 - Psest_0194 0.60 +2.5 190,662 - Psest_0194 0.60 -1.2 190,662 - Psest_0194 0.60 +3.3 190,662 - Psest_0194 0.60 +0.2 190,675 + Psest_0194 0.65 -3.7 190,675 + Psest_0194 0.65 +1.6 190,675 + Psest_0194 0.65 +0.5 190,675 + Psest_0194 0.65 -0.7 190,675 + Psest_0194 0.65 +1.4 190,675 + Psest_0194 0.65 +0.7 190,676 - Psest_0194 0.66 -0.8 190,676 - Psest_0194 0.66 +1.1 190,676 - Psest_0194 0.66 -2.5 190,676 - -0.0 190,676 - Psest_0194 0.66 -2.8 190,676 - Psest_0194 0.66 -3.8 190,881 + -0.5 190,881 + -1.7 190,881 + +1.1 190,884 + Psest_0195 0.10 +1.8 190,884 + Psest_0195 0.10 -1.0 190,884 + Psest_0195 0.10 -2.0 190,884 + Psest_0195 0.10 -0.9 190,884 + Psest_0195 0.10 -2.4 190,884 + Psest_0195 0.10 +0.1 190,884 + Psest_0195 0.10 +2.1 190,884 + Psest_0195 0.10 -3.5 190,884 + Psest_0195 0.10 -0.1 190,884 + Psest_0195 0.10 +2.9 190,884 + Psest_0195 0.10 -3.2 190,884 + Psest_0195 0.10 +1.0 190,884 + Psest_0195 0.10 +1.3 190,884 + Psest_0195 0.10 +1.3 190,884 + Psest_0195 0.10 -1.2 190,884 + Psest_0195 0.10 -0.9 190,885 - Psest_0195 0.11 -3.0 190,885 - Psest_0195 0.11 -1.5 190,885 - Psest_0195 0.11 -1.3 190,885 - Psest_0195 0.11 -0.4 190,885 - Psest_0195 0.11 -2.3 190,885 - Psest_0195 0.11 -0.3 190,885 - Psest_0195 0.11 -0.4 190,885 - Psest_0195 0.11 -1.0 190,885 - Psest_0195 0.11 +1.4 190,885 - Psest_0195 0.11 -2.7 190,974 - Psest_0195 0.63 -1.9 190,974 - Psest_0195 0.63 +0.6 191,089 + -1.3 191,089 + -1.4 191,089 + -0.7 191,089 + +0.3 191,089 + +0.7 191,090 - -0.8 191,090 - +0.8 191,090 - -1.8 191,222 + +2.4 191,222 + -1.7 191,222 + -1.2 191,324 - -2.7 191,358 - +0.1 191,528 + -0.7 191,528 + -1.7 191,528 + -1.5 191,528 + -1.2 191,529 - -0.8 191,529 - -0.8 191,529 - +1.4 191,865 + Psest_0196 0.36 -0.6 191,866 - Psest_0196 0.36 -3.0 191,866 - Psest_0196 0.36 -0.2 191,866 - Psest_0196 0.36 -0.1 191,866 - Psest_0196 0.36 -1.6 191,905 + Psest_0196 0.39 +1.3 191,905 + Psest_0196 0.39 -0.2 191,905 + Psest_0196 0.39 +2.0 191,905 + Psest_0196 0.39 -0.7 191,905 + Psest_0196 0.39 -2.5 191,906 - Psest_0196 0.39 +1.6
Or see this region's nucleotide sequence