Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1952 and Psest_1953 are separated by 5 nucleotides Psest_1953 and Psest_1954 are separated by 163 nucleotides Psest_1954 and Psest_1955 overlap by 4 nucleotides Psest_1955 and Psest_1956 are separated by 14 nucleotides
Psest_1952: Psest_1952 - hypothetical protein, at 2,069,668 to 2,070,759
_1952
Psest_1953: Psest_1953 - deoxyguanosinetriphosphate triphosphohydrolase, putative, at 2,070,765 to 2,072,096
_1953
Psest_1954: Psest_1954 - hypothetical protein, at 2,072,260 to 2,072,580
_1954
Psest_1955: Psest_1955 - Predicted membrane protein, at 2,072,577 to 2,072,957
_1955
Psest_1956: Psest_1956 - Uncharacterized conserved protein, at 2,072,972 to 2,073,373
_1956
Position (kb)
2070
2071
2072
2073 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2069.990 kb on + strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952 at 2069.991 kb on - strand, within Psest_1952 at 2070.124 kb on - strand, within Psest_1952 at 2070.161 kb on + strand, within Psest_1952 at 2070.252 kb on - strand, within Psest_1952 at 2070.252 kb on - strand, within Psest_1952 at 2070.351 kb on + strand, within Psest_1952 at 2070.386 kb on - strand, within Psest_1952 at 2070.468 kb on - strand, within Psest_1952 at 2070.561 kb on + strand, within Psest_1952 at 2070.814 kb on + strand at 2070.814 kb on + strand at 2070.988 kb on - strand, within Psest_1953 at 2070.988 kb on - strand, within Psest_1953 at 2070.988 kb on - strand, within Psest_1953 at 2071.004 kb on - strand, within Psest_1953 at 2071.078 kb on - strand, within Psest_1953 at 2071.210 kb on - strand, within Psest_1953 at 2071.210 kb on - strand, within Psest_1953 at 2071.210 kb on - strand, within Psest_1953 at 2071.258 kb on + strand, within Psest_1953 at 2071.258 kb on + strand, within Psest_1953 at 2071.259 kb on - strand, within Psest_1953 at 2071.259 kb on - strand, within Psest_1953 at 2071.281 kb on + strand, within Psest_1953 at 2071.282 kb on - strand, within Psest_1953 at 2071.282 kb on - strand, within Psest_1953 at 2071.282 kb on - strand, within Psest_1953 at 2071.388 kb on + strand, within Psest_1953 at 2071.388 kb on + strand, within Psest_1953 at 2071.389 kb on - strand, within Psest_1953 at 2071.393 kb on + strand, within Psest_1953 at 2071.393 kb on + strand, within Psest_1953 at 2071.393 kb on + strand, within Psest_1953 at 2071.394 kb on - strand, within Psest_1953 at 2071.546 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.551 kb on + strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.552 kb on - strand, within Psest_1953 at 2071.555 kb on + strand, within Psest_1953 at 2071.555 kb on + strand, within Psest_1953 at 2071.556 kb on - strand, within Psest_1953 at 2071.556 kb on - strand, within Psest_1953 at 2071.556 kb on - strand, within Psest_1953 at 2071.556 kb on - strand, within Psest_1953 at 2071.573 kb on + strand, within Psest_1953 at 2071.573 kb on + strand, within Psest_1953 at 2071.573 kb on + strand, within Psest_1953 at 2071.574 kb on - strand, within Psest_1953 at 2071.574 kb on - strand, within Psest_1953 at 2071.574 kb on - strand, within Psest_1953 at 2071.574 kb on - strand, within Psest_1953 at 2071.606 kb on + strand, within Psest_1953 at 2071.851 kb on + strand, within Psest_1953 at 2071.852 kb on - strand, within Psest_1953 at 2071.852 kb on - strand, within Psest_1953 at 2071.852 kb on - strand, within Psest_1953 at 2071.919 kb on + strand, within Psest_1953 at 2071.920 kb on - strand, within Psest_1953 at 2071.925 kb on + strand, within Psest_1953 at 2071.926 kb on - strand, within Psest_1953 at 2072.045 kb on + strand at 2072.046 kb on - strand at 2072.076 kb on - strand at 2072.138 kb on - strand at 2072.138 kb on - strand at 2072.143 kb on - strand at 2072.143 kb on - strand at 2072.262 kb on - strand at 2072.338 kb on + strand, within Psest_1954 at 2072.339 kb on - strand, within Psest_1954 at 2072.372 kb on - strand, within Psest_1954 at 2072.489 kb on + strand, within Psest_1954 at 2072.489 kb on + strand, within Psest_1954 at 2072.490 kb on - strand, within Psest_1954 at 2072.490 kb on - strand, within Psest_1954 at 2072.567 kb on - strand at 2072.685 kb on + strand, within Psest_1955 at 2072.685 kb on + strand, within Psest_1955 at 2072.695 kb on + strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.696 kb on - strand, within Psest_1955 at 2072.872 kb on - strand, within Psest_1955 at 2072.872 kb on - strand, within Psest_1955 at 2072.872 kb on - strand, within Psest_1955 at 2072.872 kb on - strand, within Psest_1955 at 2072.872 kb on - strand, within Psest_1955 at 2072.961 kb on + strand at 2072.962 kb on - strand at 2073.043 kb on + strand, within Psest_1956 at 2073.044 kb on - strand, within Psest_1956 at 2073.044 kb on - strand, within Psest_1956 at 2073.051 kb on + strand, within Psest_1956 at 2073.051 kb on + strand, within Psest_1956 at 2073.051 kb on + strand, within Psest_1956 at 2073.051 kb on + strand, within Psest_1956 at 2073.051 kb on + strand, within Psest_1956 at 2073.052 kb on - strand, within Psest_1956 at 2073.052 kb on - strand, within Psest_1956 at 2073.094 kb on + strand, within Psest_1956 at 2073.094 kb on + strand, within Psest_1956 at 2073.095 kb on - strand, within Psest_1956 at 2073.095 kb on - strand, within Psest_1956
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 2,069,990 + Psest_1952 0.29 -2.4 2,069,991 - Psest_1952 0.30 +0.0 2,069,991 - Psest_1952 0.30 -2.0 2,069,991 - Psest_1952 0.30 -1.8 2,070,124 - Psest_1952 0.42 +2.5 2,070,161 + Psest_1952 0.45 +0.8 2,070,252 - Psest_1952 0.53 +0.3 2,070,252 - Psest_1952 0.53 +0.6 2,070,351 + Psest_1952 0.63 +0.8 2,070,386 - Psest_1952 0.66 +3.9 2,070,468 - Psest_1952 0.73 -0.7 2,070,561 + Psest_1952 0.82 +0.9 2,070,814 + -1.6 2,070,814 + -1.3 2,070,988 - Psest_1953 0.17 +1.3 2,070,988 - Psest_1953 0.17 +0.9 2,070,988 - Psest_1953 0.17 -2.5 2,071,004 - Psest_1953 0.18 +0.6 2,071,078 - Psest_1953 0.23 -1.9 2,071,210 - Psest_1953 0.33 +2.7 2,071,210 - Psest_1953 0.33 -3.9 2,071,210 - Psest_1953 0.33 +0.4 2,071,258 + Psest_1953 0.37 -2.2 2,071,258 + Psest_1953 0.37 +0.0 2,071,259 - Psest_1953 0.37 +2.9 2,071,259 - Psest_1953 0.37 -0.7 2,071,281 + Psest_1953 0.39 -1.7 2,071,282 - Psest_1953 0.39 +0.4 2,071,282 - Psest_1953 0.39 -3.0 2,071,282 - Psest_1953 0.39 +0.9 2,071,388 + Psest_1953 0.47 -0.9 2,071,388 + Psest_1953 0.47 -1.2 2,071,389 - Psest_1953 0.47 -0.4 2,071,393 + Psest_1953 0.47 -0.7 2,071,393 + Psest_1953 0.47 -2.2 2,071,393 + Psest_1953 0.47 -1.9 2,071,394 - Psest_1953 0.47 -1.3 2,071,546 + Psest_1953 0.59 -0.0 2,071,551 + Psest_1953 0.59 -1.9 2,071,551 + Psest_1953 0.59 -2.9 2,071,551 + Psest_1953 0.59 -2.4 2,071,551 + Psest_1953 0.59 -1.4 2,071,551 + Psest_1953 0.59 -2.5 2,071,551 + Psest_1953 0.59 +0.5 2,071,551 + Psest_1953 0.59 -3.6 2,071,551 + Psest_1953 0.59 -0.1 2,071,551 + Psest_1953 0.59 -3.0 2,071,551 + Psest_1953 0.59 -3.5 2,071,552 - Psest_1953 0.59 -3.8 2,071,552 - Psest_1953 0.59 -1.3 2,071,552 - Psest_1953 0.59 +0.5 2,071,552 - Psest_1953 0.59 -3.9 2,071,552 - Psest_1953 0.59 -0.7 2,071,552 - Psest_1953 0.59 -0.4 2,071,555 + Psest_1953 0.59 -0.4 2,071,555 + Psest_1953 0.59 -2.2 2,071,556 - Psest_1953 0.59 -3.9 2,071,556 - Psest_1953 0.59 -1.4 2,071,556 - Psest_1953 0.59 -1.0 2,071,556 - Psest_1953 0.59 -2.7 2,071,573 + Psest_1953 0.61 -1.3 2,071,573 + Psest_1953 0.61 -3.1 2,071,573 + Psest_1953 0.61 +1.7 2,071,574 - Psest_1953 0.61 -3.0 2,071,574 - Psest_1953 0.61 -3.7 2,071,574 - Psest_1953 0.61 -0.1 2,071,574 - Psest_1953 0.61 -0.8 2,071,606 + Psest_1953 0.63 +0.2 2,071,851 + Psest_1953 0.82 -1.0 2,071,852 - Psest_1953 0.82 -0.9 2,071,852 - Psest_1953 0.82 -0.7 2,071,852 - Psest_1953 0.82 +0.8 2,071,919 + Psest_1953 0.87 -2.7 2,071,920 - Psest_1953 0.87 +0.8 2,071,925 + Psest_1953 0.87 -1.0 2,071,926 - Psest_1953 0.87 -0.1 2,072,045 + +0.9 2,072,046 - -1.3 2,072,076 - -1.3 2,072,138 - +1.6 2,072,138 - -2.0 2,072,143 - +1.8 2,072,143 - +0.1 2,072,262 - -0.9 2,072,338 + Psest_1954 0.24 +0.1 2,072,339 - Psest_1954 0.25 +0.4 2,072,372 - Psest_1954 0.35 -2.6 2,072,489 + Psest_1954 0.71 -1.1 2,072,489 + Psest_1954 0.71 -2.3 2,072,490 - Psest_1954 0.72 +1.4 2,072,490 - Psest_1954 0.72 +1.2 2,072,567 - +1.3 2,072,685 + Psest_1955 0.28 +1.2 2,072,685 + Psest_1955 0.28 -1.6 2,072,695 + Psest_1955 0.31 -0.2 2,072,696 - Psest_1955 0.31 +0.4 2,072,696 - Psest_1955 0.31 +2.2 2,072,696 - Psest_1955 0.31 -2.3 2,072,696 - Psest_1955 0.31 +1.5 2,072,696 - Psest_1955 0.31 -0.9 2,072,696 - Psest_1955 0.31 -0.1 2,072,696 - Psest_1955 0.31 -1.3 2,072,872 - Psest_1955 0.77 +1.1 2,072,872 - Psest_1955 0.77 +1.5 2,072,872 - Psest_1955 0.77 -1.4 2,072,872 - Psest_1955 0.77 -2.7 2,072,872 - Psest_1955 0.77 -0.1 2,072,961 + -4.3 2,072,962 - +0.1 2,073,043 + Psest_1956 0.18 -3.0 2,073,044 - Psest_1956 0.18 -0.6 2,073,044 - Psest_1956 0.18 +1.8 2,073,051 + Psest_1956 0.20 +1.5 2,073,051 + Psest_1956 0.20 -1.7 2,073,051 + Psest_1956 0.20 +0.9 2,073,051 + Psest_1956 0.20 +1.6 2,073,051 + Psest_1956 0.20 +1.4 2,073,052 - Psest_1956 0.20 +1.1 2,073,052 - Psest_1956 0.20 +2.0 2,073,094 + Psest_1956 0.30 +1.2 2,073,094 + Psest_1956 0.30 -0.2 2,073,095 - Psest_1956 0.31 +0.2 2,073,095 - Psest_1956 0.31 -0.9
Or see this region's nucleotide sequence