Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1802 and Psest_1803 are separated by 248 nucleotides Psest_1803 and Psest_1804 are separated by 384 nucleotides
Psest_1802: Psest_1802 - Spermidine synthase, at 1,897,906 to 1,898,655
_1802
Psest_1803: Psest_1803 - Methyl-accepting chemotaxis protein, at 1,898,904 to 1,901,066
_1803
Psest_1804: Psest_1804 - Superfamily II DNA and RNA helicases, at 1,901,451 to 1,903,121
_1804
Position (kb)
1898
1899
1900
1901
1902 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1897.965 kb on - strand at 1898.024 kb on + strand, within Psest_1802 at 1898.025 kb on - strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.121 kb on - strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.653 kb on + strand at 1898.654 kb on - strand at 1898.838 kb on - strand at 1898.941 kb on + strand at 1898.976 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.025 kb on - strand at 1899.025 kb on - strand at 1899.025 kb on - strand at 1899.076 kb on + strand at 1899.077 kb on - strand at 1899.092 kb on + strand at 1899.402 kb on + strand, within Psest_1803 at 1899.402 kb on + strand, within Psest_1803 at 1899.402 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.525 kb on + strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.734 kb on - strand, within Psest_1803 at 1899.774 kb on + strand, within Psest_1803 at 1899.891 kb on + strand, within Psest_1803 at 1899.930 kb on + strand, within Psest_1803 at 1899.931 kb on - strand, within Psest_1803 at 1899.931 kb on - strand, within Psest_1803 at 1899.931 kb on - strand, within Psest_1803 at 1899.981 kb on + strand, within Psest_1803 at 1899.981 kb on + strand, within Psest_1803 at 1899.981 kb on + strand, within Psest_1803 at 1899.981 kb on + strand, within Psest_1803 at 1899.982 kb on - strand, within Psest_1803 at 1899.982 kb on - strand, within Psest_1803 at 1899.998 kb on + strand, within Psest_1803 at 1899.998 kb on + strand, within Psest_1803 at 1900.008 kb on - strand, within Psest_1803 at 1900.027 kb on + strand, within Psest_1803 at 1900.027 kb on + strand, within Psest_1803 at 1900.027 kb on + strand, within Psest_1803 at 1900.034 kb on - strand, within Psest_1803 at 1900.034 kb on - strand, within Psest_1803 at 1900.085 kb on + strand, within Psest_1803 at 1900.232 kb on - strand, within Psest_1803 at 1900.232 kb on - strand, within Psest_1803 at 1900.327 kb on + strand, within Psest_1803 at 1900.361 kb on - strand, within Psest_1803 at 1900.805 kb on - strand, within Psest_1803 at 1901.011 kb on + strand at 1901.011 kb on + strand at 1901.011 kb on + strand at 1901.011 kb on + strand at 1901.012 kb on - strand at 1901.012 kb on - strand at 1901.012 kb on - strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.107 kb on + strand at 1901.108 kb on - strand at 1901.108 kb on - strand at 1901.108 kb on - strand at 1901.108 kb on - strand at 1901.235 kb on - strand at 1901.235 kb on - strand at 1901.343 kb on + strand at 1901.344 kb on - strand at 1901.666 kb on + strand, within Psest_1804 at 1901.739 kb on + strand, within Psest_1804 at 1901.779 kb on - strand, within Psest_1804
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,897,965 - +1.3 1,898,024 + Psest_1802 0.16 -2.8 1,898,025 - Psest_1802 0.16 +0.7 1,898,059 + Psest_1802 0.20 +0.5 1,898,059 + Psest_1802 0.20 -0.5 1,898,121 - Psest_1802 0.29 +1.0 1,898,257 + Psest_1802 0.47 -1.2 1,898,257 + Psest_1802 0.47 +3.5 1,898,257 + Psest_1802 0.47 -0.1 1,898,257 + Psest_1802 0.47 +0.5 1,898,257 + Psest_1802 0.47 +4.0 1,898,258 - Psest_1802 0.47 -2.2 1,898,258 - Psest_1802 0.47 +2.3 1,898,258 - Psest_1802 0.47 -1.7 1,898,258 - Psest_1802 0.47 -3.5 1,898,258 - Psest_1802 0.47 +0.0 1,898,330 - Psest_1802 0.57 +0.8 1,898,330 - Psest_1802 0.57 -1.6 1,898,653 + +0.3 1,898,654 - +0.1 1,898,838 - +0.3 1,898,941 + +0.8 1,898,976 - -0.1 1,898,987 - -2.8 1,898,987 - -1.2 1,898,987 - -1.4 1,898,987 - +0.1 1,898,987 - -1.6 1,898,987 - +0.1 1,899,024 + +0.9 1,899,024 + -1.5 1,899,024 + -2.2 1,899,024 + -2.3 1,899,025 - +0.2 1,899,025 - -3.5 1,899,025 - -0.8 1,899,076 + +0.2 1,899,077 - +1.0 1,899,092 + +0.5 1,899,402 + Psest_1803 0.23 -1.4 1,899,402 + Psest_1803 0.23 -0.6 1,899,402 + Psest_1803 0.23 +1.7 1,899,500 + Psest_1803 0.28 +0.5 1,899,500 + Psest_1803 0.28 -0.7 1,899,500 + Psest_1803 0.28 -0.1 1,899,501 - Psest_1803 0.28 +0.4 1,899,501 - Psest_1803 0.28 -0.7 1,899,501 - Psest_1803 0.28 +0.3 1,899,501 - Psest_1803 0.28 +2.0 1,899,501 - Psest_1803 0.28 +0.3 1,899,501 - Psest_1803 0.28 -0.9 1,899,525 + Psest_1803 0.29 -2.4 1,899,526 - Psest_1803 0.29 -0.1 1,899,526 - Psest_1803 0.29 +2.5 1,899,526 - Psest_1803 0.29 +0.0 1,899,526 - Psest_1803 0.29 +0.4 1,899,526 - Psest_1803 0.29 +2.1 1,899,526 - Psest_1803 0.29 -1.6 1,899,734 - Psest_1803 0.38 -1.4 1,899,774 + Psest_1803 0.40 -2.7 1,899,891 + Psest_1803 0.46 +2.9 1,899,930 + Psest_1803 0.47 +1.3 1,899,931 - Psest_1803 0.47 -1.0 1,899,931 - Psest_1803 0.47 -0.7 1,899,931 - Psest_1803 0.47 -1.2 1,899,981 + Psest_1803 0.50 +1.0 1,899,981 + Psest_1803 0.50 -1.1 1,899,981 + Psest_1803 0.50 -3.1 1,899,981 + Psest_1803 0.50 +0.3 1,899,982 - Psest_1803 0.50 -3.2 1,899,982 - Psest_1803 0.50 -3.2 1,899,998 + Psest_1803 0.51 +0.1 1,899,998 + Psest_1803 0.51 +1.6 1,900,008 - Psest_1803 0.51 +1.7 1,900,027 + Psest_1803 0.52 +1.2 1,900,027 + Psest_1803 0.52 +0.8 1,900,027 + Psest_1803 0.52 +3.1 1,900,034 - Psest_1803 0.52 -1.0 1,900,034 - Psest_1803 0.52 -0.5 1,900,085 + Psest_1803 0.55 -0.6 1,900,232 - Psest_1803 0.61 +1.8 1,900,232 - Psest_1803 0.61 +1.6 1,900,327 + Psest_1803 0.66 -3.3 1,900,361 - Psest_1803 0.67 +0.1 1,900,805 - Psest_1803 0.88 -2.0 1,901,011 + +2.3 1,901,011 + +0.6 1,901,011 + +1.4 1,901,011 + +2.1 1,901,012 - -1.7 1,901,012 - -2.1 1,901,012 - +3.9 1,901,107 + -1.9 1,901,107 + -0.8 1,901,107 + -1.8 1,901,107 + -2.8 1,901,107 + -4.0 1,901,107 + +0.6 1,901,107 + -0.1 1,901,107 + -0.7 1,901,108 - +1.2 1,901,108 - -1.3 1,901,108 - -1.5 1,901,108 - +1.0 1,901,235 - +0.3 1,901,235 - -0.1 1,901,343 + -2.0 1,901,344 - +1.9 1,901,666 + Psest_1804 0.13 +0.3 1,901,739 + Psest_1804 0.17 +0.3 1,901,779 - Psest_1804 0.20 -0.1
Or see this region's nucleotide sequence