Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0163 and Psest_0164 are separated by 2 nucleotides Psest_0164 and Psest_0165 are separated by 64 nucleotides Psest_0165 and Psest_0166 are separated by 51 nucleotides Psest_0166 and Psest_0167 are separated by 213 nucleotides Psest_0167 and Psest_0168 are separated by 1 nucleotides
Psest_0163: Psest_0163 - Rhodanese-related sulfurtransferase, at 162,307 to 162,720
_0163
Psest_0164: Psest_0164 - Glutaredoxin, GrxC family, at 162,723 to 162,977
_0164
Psest_0165: Psest_0165 - protein-export chaperone SecB, at 163,042 to 163,536
_0165
Psest_0166: Psest_0166 - NADPH:quinone reductase and related Zn-dependent oxidoreductases, at 163,588 to 164,568
_0166
Psest_0167: Psest_0167 - Flagellar basal body-associated protein, at 164,782 to 165,183
_0167
Psest_0168: Psest_0168 - Uncharacterized conserved protein, at 165,185 to 165,634
_0168
Position (kb) 163
164
165 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.718 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.741 kb on + strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.742 kb on - strand at 162.743 kb on + strand at 162.743 kb on + strand at 162.807 kb on + strand, within Psest_0164 at 163.028 kb on - strand at 163.028 kb on - strand at 163.056 kb on + strand at 163.057 kb on - strand at 163.117 kb on + strand, within Psest_0165 at 163.118 kb on - strand, within Psest_0165 at 163.118 kb on - strand, within Psest_0165 at 163.477 kb on + strand, within Psest_0165 at 163.477 kb on + strand, within Psest_0165 at 163.477 kb on + strand, within Psest_0165 at 163.735 kb on - strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.931 kb on + strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.932 kb on - strand, within Psest_0166 at 163.998 kb on - strand, within Psest_0166 at 163.998 kb on - strand, within Psest_0166 at 163.998 kb on - strand, within Psest_0166 at 164.175 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.198 kb on + strand, within Psest_0166 at 164.199 kb on - strand, within Psest_0166 at 164.199 kb on - strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.417 kb on + strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.418 kb on - strand, within Psest_0166 at 164.626 kb on - strand at 164.644 kb on + strand at 164.644 kb on + strand at 164.645 kb on - strand at 164.645 kb on - strand at 164.695 kb on + strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.696 kb on - strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.697 kb on + strand at 164.698 kb on - strand at 164.788 kb on + strand at 164.875 kb on + strand, within Psest_0167 at 164.876 kb on - strand, within Psest_0167 at 164.878 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.908 kb on + strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.909 kb on - strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.932 kb on + strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.933 kb on - strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.935 kb on + strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 164.936 kb on - strand, within Psest_0167 at 165.135 kb on + strand, within Psest_0167 at 165.136 kb on - strand, within Psest_0167 at 165.486 kb on + strand, within Psest_0168 at 165.486 kb on + strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.487 kb on - strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.514 kb on + strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.515 kb on - strand, within Psest_0168 at 165.541 kb on - strand, within Psest_0168 at 165.549 kb on - strand, within Psest_0168
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 162,718 + +0.5 162,718 + +0.9 162,718 + -3.3 162,718 + +2.6 162,718 + +0.4 162,718 + -0.0 162,718 + +1.2 162,718 + -1.6 162,741 + +0.4 162,741 + -0.1 162,741 + -3.9 162,741 + -0.6 162,741 + +0.4 162,741 + -1.3 162,741 + -1.0 162,741 + +1.7 162,741 + -1.6 162,741 + +1.0 162,741 + +0.5 162,741 + -2.1 162,741 + -3.9 162,741 + -1.1 162,741 + -0.8 162,741 + -2.2 162,741 + -0.6 162,741 + +1.0 162,741 + -0.1 162,741 + -3.4 162,741 + -2.9 162,742 - +5.4 162,742 - +1.0 162,742 - +1.0 162,742 - +2.0 162,742 - -2.3 162,742 - -2.5 162,742 - -0.6 162,743 + +0.4 162,743 + +1.8 162,807 + Psest_0164 0.33 +1.1 163,028 - +2.0 163,028 - +1.0 163,056 + +1.0 163,057 - +2.0 163,117 + Psest_0165 0.15 +1.0 163,118 - Psest_0165 0.15 +2.0 163,118 - Psest_0165 0.15 -0.0 163,477 + Psest_0165 0.88 -1.6 163,477 + Psest_0165 0.88 -1.6 163,477 + Psest_0165 0.88 -1.7 163,735 - Psest_0166 0.15 -1.6 163,931 + Psest_0166 0.35 +0.7 163,931 + Psest_0166 0.35 -2.3 163,931 + Psest_0166 0.35 -0.4 163,931 + Psest_0166 0.35 -2.2 163,931 + Psest_0166 0.35 +0.4 163,931 + Psest_0166 0.35 +0.7 163,931 + Psest_0166 0.35 -2.4 163,931 + Psest_0166 0.35 +0.0 163,931 + Psest_0166 0.35 +2.7 163,931 + Psest_0166 0.35 -0.0 163,931 + Psest_0166 0.35 -1.8 163,931 + Psest_0166 0.35 -3.0 163,932 - Psest_0166 0.35 -3.0 163,932 - Psest_0166 0.35 -1.0 163,932 - Psest_0166 0.35 -0.0 163,932 - Psest_0166 0.35 -1.8 163,932 - Psest_0166 0.35 +0.1 163,932 - Psest_0166 0.35 -1.3 163,998 - Psest_0166 0.42 +2.0 163,998 - Psest_0166 0.42 +1.4 163,998 - Psest_0166 0.42 -2.1 164,175 + Psest_0166 0.60 +0.2 164,198 + Psest_0166 0.62 -2.1 164,198 + Psest_0166 0.62 -0.7 164,198 + Psest_0166 0.62 -3.1 164,198 + Psest_0166 0.62 -0.3 164,198 + Psest_0166 0.62 -2.5 164,198 + Psest_0166 0.62 -1.2 164,199 - Psest_0166 0.62 -1.9 164,199 - Psest_0166 0.62 -2.0 164,417 + Psest_0166 0.85 -0.3 164,417 + Psest_0166 0.85 -1.5 164,417 + Psest_0166 0.85 +0.3 164,417 + Psest_0166 0.85 -1.8 164,417 + Psest_0166 0.85 -0.7 164,418 - Psest_0166 0.85 +1.0 164,418 - Psest_0166 0.85 -0.4 164,418 - Psest_0166 0.85 +0.9 164,418 - Psest_0166 0.85 -1.4 164,418 - Psest_0166 0.85 -2.7 164,626 - -1.6 164,644 + +2.8 164,644 + -0.3 164,645 - +2.4 164,645 - -2.0 164,695 + -0.6 164,696 - -2.1 164,696 - -3.8 164,696 - -1.5 164,696 - +1.7 164,697 + -1.7 164,697 + -0.6 164,697 + -3.5 164,697 + -1.3 164,698 - -0.8 164,788 + -2.1 164,875 + Psest_0167 0.23 +1.8 164,876 - Psest_0167 0.23 +3.3 164,878 + Psest_0167 0.24 -0.2 164,908 + Psest_0167 0.31 -2.6 164,908 + Psest_0167 0.31 -3.3 164,908 + Psest_0167 0.31 -0.0 164,908 + Psest_0167 0.31 +0.6 164,908 + Psest_0167 0.31 +0.6 164,908 + Psest_0167 0.31 -2.1 164,908 + Psest_0167 0.31 -1.3 164,908 + Psest_0167 0.31 -1.2 164,908 + Psest_0167 0.31 +0.2 164,908 + Psest_0167 0.31 -1.6 164,908 + Psest_0167 0.31 +3.2 164,908 + Psest_0167 0.31 +0.5 164,909 - Psest_0167 0.32 -1.1 164,909 - Psest_0167 0.32 +6.3 164,909 - Psest_0167 0.32 -0.5 164,909 - Psest_0167 0.32 -1.2 164,909 - Psest_0167 0.32 -0.3 164,909 - Psest_0167 0.32 +1.1 164,909 - Psest_0167 0.32 +1.5 164,932 + Psest_0167 0.37 +0.4 164,932 + Psest_0167 0.37 -0.7 164,932 + Psest_0167 0.37 +0.1 164,932 + Psest_0167 0.37 -0.6 164,932 + Psest_0167 0.37 -1.5 164,933 - Psest_0167 0.38 +3.3 164,933 - Psest_0167 0.38 +0.7 164,933 - Psest_0167 0.38 -2.1 164,935 + Psest_0167 0.38 +0.4 164,935 + Psest_0167 0.38 -1.0 164,935 + Psest_0167 0.38 -3.3 164,935 + Psest_0167 0.38 +2.5 164,935 + Psest_0167 0.38 +0.6 164,935 + Psest_0167 0.38 -1.5 164,935 + Psest_0167 0.38 +0.2 164,936 - Psest_0167 0.38 -0.1 164,936 - Psest_0167 0.38 -2.3 164,936 - Psest_0167 0.38 +3.3 164,936 - Psest_0167 0.38 +0.4 164,936 - Psest_0167 0.38 -0.9 164,936 - Psest_0167 0.38 -2.7 164,936 - Psest_0167 0.38 -1.3 164,936 - Psest_0167 0.38 +0.1 164,936 - Psest_0167 0.38 -0.3 164,936 - Psest_0167 0.38 +2.3 165,135 + Psest_0167 0.88 +0.9 165,136 - Psest_0167 0.88 +0.6 165,486 + Psest_0168 0.67 +0.8 165,486 + Psest_0168 0.67 -1.8 165,487 - Psest_0168 0.67 +4.2 165,487 - Psest_0168 0.67 +5.1 165,487 - Psest_0168 0.67 -1.9 165,487 - Psest_0168 0.67 +1.6 165,487 - Psest_0168 0.67 +0.6 165,514 + Psest_0168 0.73 +1.2 165,514 + Psest_0168 0.73 -1.6 165,514 + Psest_0168 0.73 +0.9 165,515 - Psest_0168 0.73 -1.1 165,515 - Psest_0168 0.73 +0.3 165,541 - Psest_0168 0.79 -1.9 165,549 - Psest_0168 0.81 -1.7
Or see this region's nucleotide sequence