Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1219 and Psest_1220 are separated by 3 nucleotides Psest_1220 and Psest_1221 overlap by 4 nucleotides Psest_1221 and Psest_1222 are separated by 7 nucleotides
Psest_1219: Psest_1219 - Glutathione S-transferase, at 1,326,680 to 1,327,618
_1219
Psest_1220: Psest_1220 - hypothetical protein, at 1,327,622 to 1,328,464
_1220
Psest_1221: Psest_1221 - Uncharacterized conserved protein, at 1,328,461 to 1,329,279
_1221
Psest_1222: Psest_1222 - Nucleoid-associated protein, at 1,329,287 to 1,330,294
_1222
Position (kb)
1328
1329
1330 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1327.515 kb on + strand, within Psest_1219 at 1327.516 kb on - strand, within Psest_1219 at 1327.785 kb on - strand, within Psest_1220 at 1327.893 kb on + strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.984 kb on - strand, within Psest_1220 at 1327.998 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.203 kb on - strand, within Psest_1220 at 1328.203 kb on - strand, within Psest_1220 at 1328.435 kb on + strand at 1328.436 kb on - strand at 1328.594 kb on - strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.601 kb on - strand, within Psest_1221 at 1328.774 kb on + strand, within Psest_1221 at 1328.775 kb on - strand, within Psest_1221 at 1328.900 kb on + strand, within Psest_1221 at 1328.900 kb on + strand, within Psest_1221 at 1328.900 kb on + strand, within Psest_1221 at 1328.903 kb on + strand, within Psest_1221 at 1328.903 kb on + strand, within Psest_1221 at 1328.903 kb on + strand, within Psest_1221 at 1328.903 kb on + strand, within Psest_1221 at 1328.903 kb on + strand, within Psest_1221 at 1328.904 kb on - strand, within Psest_1221 at 1328.904 kb on - strand, within Psest_1221 at 1329.091 kb on + strand, within Psest_1221 at 1329.091 kb on + strand, within Psest_1221 at 1329.513 kb on + strand, within Psest_1222 at 1329.601 kb on - strand, within Psest_1222 at 1329.664 kb on - strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.789 kb on + strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.790 kb on - strand, within Psest_1222 at 1329.942 kb on + strand, within Psest_1222 at 1329.942 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.077 kb on + strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.078 kb on - strand, within Psest_1222 at 1330.158 kb on + strand, within Psest_1222 at 1330.158 kb on + strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222 at 1330.159 kb on - strand, within Psest_1222
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,327,515 + Psest_1219 0.89 +2.4 1,327,516 - Psest_1219 0.89 -1.5 1,327,785 - Psest_1220 0.19 +0.2 1,327,893 + Psest_1220 0.32 +4.2 1,327,894 - Psest_1220 0.32 +0.7 1,327,894 - Psest_1220 0.32 +0.1 1,327,894 - Psest_1220 0.32 +2.1 1,327,894 - Psest_1220 0.32 -0.0 1,327,984 - Psest_1220 0.43 -2.4 1,327,998 + Psest_1220 0.45 +3.1 1,328,088 + Psest_1220 0.55 -1.3 1,328,088 + Psest_1220 0.55 -2.1 1,328,088 + Psest_1220 0.55 +0.3 1,328,088 + Psest_1220 0.55 -2.3 1,328,088 + Psest_1220 0.55 -0.7 1,328,089 - Psest_1220 0.55 -0.8 1,328,089 - Psest_1220 0.55 -2.1 1,328,089 - Psest_1220 0.55 +0.8 1,328,089 - Psest_1220 0.55 -0.6 1,328,089 - Psest_1220 0.55 +2.7 1,328,089 - Psest_1220 0.55 -1.4 1,328,202 + Psest_1220 0.69 +1.4 1,328,202 + Psest_1220 0.69 +0.6 1,328,202 + Psest_1220 0.69 -1.0 1,328,203 - Psest_1220 0.69 +0.8 1,328,203 - Psest_1220 0.69 -0.4 1,328,435 + -1.1 1,328,436 - +1.9 1,328,594 - Psest_1221 0.16 -0.8 1,328,600 + Psest_1221 0.17 +0.6 1,328,600 + Psest_1221 0.17 +1.0 1,328,600 + Psest_1221 0.17 +1.1 1,328,601 - Psest_1221 0.17 -2.3 1,328,774 + Psest_1221 0.38 +0.6 1,328,775 - Psest_1221 0.38 +2.9 1,328,900 + Psest_1221 0.54 +2.7 1,328,900 + Psest_1221 0.54 +1.4 1,328,900 + Psest_1221 0.54 -1.4 1,328,903 + Psest_1221 0.54 +0.5 1,328,903 + Psest_1221 0.54 -2.5 1,328,903 + Psest_1221 0.54 +0.6 1,328,903 + Psest_1221 0.54 +5.6 1,328,903 + Psest_1221 0.54 +0.1 1,328,904 - Psest_1221 0.54 -1.0 1,328,904 - Psest_1221 0.54 +1.3 1,329,091 + Psest_1221 0.77 +1.2 1,329,091 + Psest_1221 0.77 +1.0 1,329,513 + Psest_1222 0.22 +1.2 1,329,601 - Psest_1222 0.31 +0.7 1,329,664 - Psest_1222 0.37 -1.8 1,329,789 + Psest_1222 0.50 +1.5 1,329,789 + Psest_1222 0.50 -2.1 1,329,789 + Psest_1222 0.50 -2.3 1,329,789 + Psest_1222 0.50 -0.9 1,329,789 + Psest_1222 0.50 -1.5 1,329,789 + Psest_1222 0.50 -0.9 1,329,789 + Psest_1222 0.50 +2.4 1,329,789 + Psest_1222 0.50 -1.6 1,329,789 + Psest_1222 0.50 +4.8 1,329,789 + Psest_1222 0.50 +0.2 1,329,789 + Psest_1222 0.50 -2.6 1,329,789 + Psest_1222 0.50 +1.0 1,329,790 - Psest_1222 0.50 -1.5 1,329,790 - Psest_1222 0.50 +0.9 1,329,790 - Psest_1222 0.50 +2.0 1,329,790 - Psest_1222 0.50 -0.8 1,329,790 - Psest_1222 0.50 +0.4 1,329,790 - Psest_1222 0.50 -1.4 1,329,790 - Psest_1222 0.50 -0.2 1,329,790 - Psest_1222 0.50 +0.9 1,329,790 - Psest_1222 0.50 -0.1 1,329,790 - Psest_1222 0.50 +0.0 1,329,790 - Psest_1222 0.50 +0.2 1,329,942 + Psest_1222 0.65 +2.0 1,329,942 + Psest_1222 0.65 -0.8 1,330,077 + Psest_1222 0.78 -1.7 1,330,077 + Psest_1222 0.78 +3.0 1,330,077 + Psest_1222 0.78 +0.7 1,330,077 + Psest_1222 0.78 -2.0 1,330,077 + Psest_1222 0.78 +1.3 1,330,077 + Psest_1222 0.78 +0.8 1,330,078 - Psest_1222 0.78 -0.0 1,330,078 - Psest_1222 0.78 +0.0 1,330,078 - Psest_1222 0.78 -0.6 1,330,078 - Psest_1222 0.78 +0.5 1,330,078 - Psest_1222 0.78 -1.9 1,330,078 - Psest_1222 0.78 -2.4 1,330,078 - Psest_1222 0.78 -1.5 1,330,158 + Psest_1222 0.86 -1.0 1,330,158 + Psest_1222 0.86 -0.5 1,330,159 - Psest_1222 0.87 +0.3 1,330,159 - Psest_1222 0.87 +1.2 1,330,159 - Psest_1222 0.87 -0.4 1,330,159 - Psest_1222 0.87 +2.3 1,330,159 - Psest_1222 0.87 +2.0 1,330,159 - Psest_1222 0.87 +1.6 1,330,159 - Psest_1222 0.87 +0.0 1,330,159 - Psest_1222 0.87 +0.9 1,330,159 - Psest_1222 0.87 -2.2 1,330,159 - Psest_1222 0.87 +2.7
Or see this region's nucleotide sequence