Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1204 and Psest_1205 are separated by 306 nucleotides Psest_1205 and Psest_1206 overlap by 4 nucleotides Psest_1206 and Psest_1207 are separated by 9 nucleotides
Psest_1204: Psest_1204 - Transcriptional regulators, at 1,307,408 to 1,308,163
_1204
Psest_1205: Psest_1205 - ABC-type Mn/Zn transport systems, ATPase component, at 1,308,470 to 1,309,141
_1205
Psest_1206: Psest_1206 - ABC-type Mn2+/Zn2+ transport systems, permease components, at 1,309,138 to 1,310,001
_1206
Psest_1207: Psest_1207 - ABC-type metal ion transport system, periplasmic component/surface adhesin, at 1,310,011 to 1,310,883
_1207
Position (kb)
1308
1309
1310 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1307.486 kb on + strand, within Psest_1204 at 1307.565 kb on + strand, within Psest_1204 at 1307.565 kb on + strand, within Psest_1204 at 1307.566 kb on - strand, within Psest_1204 at 1307.629 kb on - strand, within Psest_1204 at 1307.973 kb on - strand, within Psest_1204 at 1307.973 kb on - strand, within Psest_1204 at 1308.207 kb on + strand at 1308.207 kb on + strand at 1308.208 kb on - strand at 1308.287 kb on + strand at 1308.288 kb on - strand at 1308.288 kb on - strand at 1308.322 kb on - strand at 1308.343 kb on + strand at 1308.343 kb on + strand at 1308.343 kb on + strand at 1308.343 kb on + strand at 1308.344 kb on - strand at 1308.465 kb on + strand at 1308.465 kb on + strand at 1308.466 kb on - strand at 1308.466 kb on - strand at 1308.518 kb on - strand at 1308.607 kb on - strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.640 kb on + strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.641 kb on - strand, within Psest_1205 at 1308.642 kb on + strand, within Psest_1205 at 1308.642 kb on + strand, within Psest_1205 at 1308.642 kb on + strand, within Psest_1205 at 1308.642 kb on + strand, within Psest_1205 at 1308.642 kb on + strand, within Psest_1205 at 1308.643 kb on - strand, within Psest_1205 at 1308.643 kb on - strand, within Psest_1205 at 1308.677 kb on + strand, within Psest_1205 at 1309.022 kb on - strand, within Psest_1205 at 1309.367 kb on + strand, within Psest_1206 at 1309.879 kb on + strand, within Psest_1206 at 1309.879 kb on + strand, within Psest_1206 at 1309.880 kb on - strand, within Psest_1206 at 1309.880 kb on - strand, within Psest_1206 at 1309.931 kb on - strand at 1309.947 kb on - strand at 1309.980 kb on + strand at 1309.980 kb on + strand at 1309.980 kb on + strand at 1309.981 kb on - strand at 1310.005 kb on - strand at 1310.005 kb on - strand at 1310.005 kb on - strand at 1310.017 kb on + strand at 1310.027 kb on + strand at 1310.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,307,486 + Psest_1204 0.10 +2.0 1,307,565 + Psest_1204 0.21 +2.3 1,307,565 + Psest_1204 0.21 -2.4 1,307,566 - Psest_1204 0.21 -2.4 1,307,629 - Psest_1204 0.29 -2.7 1,307,973 - Psest_1204 0.75 -3.0 1,307,973 - Psest_1204 0.75 -0.9 1,308,207 + +3.4 1,308,207 + +1.5 1,308,208 - +0.5 1,308,287 + +1.7 1,308,288 - -0.0 1,308,288 - -0.0 1,308,322 - -0.2 1,308,343 + -0.3 1,308,343 + +2.1 1,308,343 + -2.4 1,308,343 + -3.0 1,308,344 - -0.9 1,308,465 + +1.0 1,308,465 + +2.0 1,308,466 - +4.5 1,308,466 - -0.1 1,308,518 - +3.3 1,308,607 - Psest_1205 0.20 +0.5 1,308,640 + Psest_1205 0.25 -0.3 1,308,640 + Psest_1205 0.25 -0.4 1,308,640 + Psest_1205 0.25 +0.1 1,308,640 + Psest_1205 0.25 -2.2 1,308,640 + Psest_1205 0.25 -0.0 1,308,640 + Psest_1205 0.25 -0.9 1,308,640 + Psest_1205 0.25 -2.0 1,308,640 + Psest_1205 0.25 -3.4 1,308,640 + Psest_1205 0.25 +0.3 1,308,640 + Psest_1205 0.25 -1.9 1,308,640 + Psest_1205 0.25 +2.0 1,308,640 + Psest_1205 0.25 -1.4 1,308,641 - Psest_1205 0.25 +1.7 1,308,641 - Psest_1205 0.25 -2.0 1,308,641 - Psest_1205 0.25 -0.6 1,308,641 - Psest_1205 0.25 +1.0 1,308,641 - Psest_1205 0.25 -1.2 1,308,641 - Psest_1205 0.25 +0.5 1,308,641 - Psest_1205 0.25 -1.4 1,308,641 - Psest_1205 0.25 +3.5 1,308,641 - Psest_1205 0.25 +0.9 1,308,641 - Psest_1205 0.25 -2.5 1,308,641 - Psest_1205 0.25 -0.4 1,308,642 + Psest_1205 0.26 -1.5 1,308,642 + Psest_1205 0.26 -3.2 1,308,642 + Psest_1205 0.26 -1.9 1,308,642 + Psest_1205 0.26 -2.6 1,308,642 + Psest_1205 0.26 -0.1 1,308,643 - Psest_1205 0.26 +0.2 1,308,643 - Psest_1205 0.26 -0.7 1,308,677 + Psest_1205 0.31 -0.1 1,309,022 - Psest_1205 0.82 -0.2 1,309,367 + Psest_1206 0.27 -0.7 1,309,879 + Psest_1206 0.86 -1.3 1,309,879 + Psest_1206 0.86 -0.1 1,309,880 - Psest_1206 0.86 +1.9 1,309,880 - Psest_1206 0.86 -0.4 1,309,931 - -0.4 1,309,947 - +0.6 1,309,980 + -1.1 1,309,980 + +0.6 1,309,980 + -0.0 1,309,981 - +0.3 1,310,005 - +2.1 1,310,005 - -1.1 1,310,005 - -1.0 1,310,017 + +0.4 1,310,027 + -0.4 1,310,028 - -2.9
Or see this region's nucleotide sequence