Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0001

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0001 and Psest_0002 are separated by 514 nucleotides Psest_0001: Psest_0001 - ribosomal protein L34, bacterial type, at 12 to 146 _0001 Psest_0002: Psest_0002 - chromosomal replication initiator protein DnaA, at 661 to 2,109 _0002 Position (kb) 0 1Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 0.300 kb on - strandat 0.300 kb on - strandat 0.315 kb on - strandat 0.315 kb on - strandat 0.401 kb on - strandat 0.401 kb on - strandat 0.423 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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300 - -0.5
300 - +0.6
315 - +2.4
315 - -3.1
401 - +0.6
401 - +1.2
423 - -2.1

Or see this region's nucleotide sequence