Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3217 and Psest_3218 are separated by 148 nucleotides Psest_3218 and Psest_3219 are separated by 130 nucleotides Psest_3219 and Psest_3221 are separated by 364 nucleotides
Psest_3217: Psest_3217 - PAS domain S-box, at 3,387,688 to 3,391,407
_3217
Psest_3218: Psest_3218 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 3,391,556 to 3,392,209
_3218
Psest_3219: Psest_3219 - Zn-dependent hydrolases, including glyoxylases, at 3,392,340 to 3,392,990
_3219
Psest_3221: Psest_3221 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II, at 3,393,355 to 3,395,040
_3221
Position (kb)
3392
3393 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3391.360 kb on + strand at 3391.360 kb on + strand at 3391.459 kb on - strand at 3391.542 kb on + strand at 3391.547 kb on + strand at 3391.547 kb on + strand at 3391.548 kb on - strand at 3391.550 kb on + strand at 3391.550 kb on + strand at 3391.557 kb on + strand at 3391.558 kb on - strand at 3391.650 kb on + strand, within Psest_3218 at 3391.692 kb on + strand, within Psest_3218 at 3391.753 kb on + strand, within Psest_3218 at 3391.763 kb on - strand, within Psest_3218 at 3391.873 kb on + strand, within Psest_3218 at 3391.873 kb on + strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.874 kb on - strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.977 kb on + strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.978 kb on - strand, within Psest_3218 at 3391.980 kb on + strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.104 kb on - strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.105 kb on + strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.106 kb on - strand, within Psest_3218 at 3392.139 kb on + strand, within Psest_3218 at 3392.140 kb on - strand, within Psest_3218 at 3392.140 kb on - strand, within Psest_3218 at 3392.153 kb on - strand at 3392.209 kb on + strand at 3392.210 kb on - strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.213 kb on + strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.214 kb on - strand at 3392.233 kb on + strand at 3392.254 kb on - strand at 3392.279 kb on - strand at 3392.279 kb on - strand at 3392.279 kb on - strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.283 kb on + strand at 3392.284 kb on - strand at 3392.305 kb on + strand at 3392.305 kb on + strand at 3392.306 kb on - strand at 3392.306 kb on - strand at 3392.438 kb on + strand, within Psest_3219 at 3392.438 kb on + strand, within Psest_3219 at 3392.438 kb on + strand, within Psest_3219 at 3392.439 kb on - strand, within Psest_3219 at 3392.439 kb on - strand, within Psest_3219 at 3392.440 kb on + strand, within Psest_3219 at 3392.440 kb on + strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.441 kb on - strand, within Psest_3219 at 3392.582 kb on + strand, within Psest_3219 at 3392.582 kb on + strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.583 kb on - strand, within Psest_3219 at 3392.671 kb on + strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3392.672 kb on - strand, within Psest_3219 at 3393.012 kb on + strand at 3393.319 kb on + strand at 3393.319 kb on + strand at 3393.320 kb on - strand at 3393.328 kb on + strand at 3393.501 kb on + strand at 3393.501 kb on + strand at 3393.501 kb on + strand at 3393.502 kb on - strand at 3393.502 kb on - strand at 3393.631 kb on + strand, within Psest_3221 at 3393.631 kb on + strand, within Psest_3221 at 3393.824 kb on + strand, within Psest_3221 at 3393.824 kb on + strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.825 kb on - strand, within Psest_3221 at 3393.827 kb on + strand, within Psest_3221 at 3393.827 kb on + strand, within Psest_3221 at 3393.828 kb on - strand, within Psest_3221 at 3393.828 kb on - strand, within Psest_3221 at 3393.838 kb on - strand, within Psest_3221 at 3393.857 kb on - strand, within Psest_3221
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 3,391,360 + +0.7 3,391,360 + +1.3 3,391,459 - -0.6 3,391,542 + +0.7 3,391,547 + +0.3 3,391,547 + +1.2 3,391,548 - +0.0 3,391,550 + +0.8 3,391,550 + -1.1 3,391,557 + +1.2 3,391,558 - +1.4 3,391,650 + Psest_3218 0.14 +0.2 3,391,692 + Psest_3218 0.21 -1.6 3,391,753 + Psest_3218 0.30 +1.5 3,391,763 - Psest_3218 0.32 -0.3 3,391,873 + Psest_3218 0.48 -2.5 3,391,873 + Psest_3218 0.48 -0.1 3,391,874 - Psest_3218 0.49 +1.4 3,391,874 - Psest_3218 0.49 -0.5 3,391,874 - Psest_3218 0.49 +0.2 3,391,874 - Psest_3218 0.49 +0.9 3,391,977 + Psest_3218 0.64 +0.5 3,391,977 + Psest_3218 0.64 -0.6 3,391,977 + Psest_3218 0.64 +0.8 3,391,977 + Psest_3218 0.64 +1.7 3,391,977 + Psest_3218 0.64 -0.7 3,391,978 - Psest_3218 0.65 +1.3 3,391,978 - Psest_3218 0.65 -0.8 3,391,978 - Psest_3218 0.65 -0.9 3,391,978 - Psest_3218 0.65 +1.4 3,391,978 - Psest_3218 0.65 +1.6 3,391,978 - Psest_3218 0.65 +0.3 3,391,978 - Psest_3218 0.65 +0.7 3,391,980 + Psest_3218 0.65 -1.4 3,392,104 - Psest_3218 0.84 -0.2 3,392,104 - Psest_3218 0.84 -1.1 3,392,104 - Psest_3218 0.84 +0.6 3,392,105 + Psest_3218 0.84 +0.3 3,392,105 + Psest_3218 0.84 -0.8 3,392,105 + Psest_3218 0.84 -2.4 3,392,105 + Psest_3218 0.84 -0.6 3,392,105 + Psest_3218 0.84 -0.7 3,392,105 + Psest_3218 0.84 +3.6 3,392,106 - Psest_3218 0.84 +0.7 3,392,106 - Psest_3218 0.84 +0.9 3,392,106 - Psest_3218 0.84 +0.0 3,392,106 - Psest_3218 0.84 -2.2 3,392,106 - Psest_3218 0.84 -0.0 3,392,106 - Psest_3218 0.84 +4.0 3,392,106 - Psest_3218 0.84 +2.5 3,392,106 - Psest_3218 0.84 -2.3 3,392,106 - Psest_3218 0.84 +1.4 3,392,106 - Psest_3218 0.84 +0.9 3,392,106 - Psest_3218 0.84 +5.3 3,392,106 - Psest_3218 0.84 -0.7 3,392,106 - Psest_3218 0.84 +0.5 3,392,106 - Psest_3218 0.84 -1.5 3,392,106 - Psest_3218 0.84 -0.8 3,392,106 - Psest_3218 0.84 -0.0 3,392,106 - Psest_3218 0.84 +0.0 3,392,106 - Psest_3218 0.84 -0.7 3,392,139 + Psest_3218 0.89 +0.9 3,392,140 - Psest_3218 0.89 -0.9 3,392,140 - Psest_3218 0.89 -0.4 3,392,153 - -0.1 3,392,209 + -0.1 3,392,210 - -1.9 3,392,213 + +0.3 3,392,213 + -0.1 3,392,213 + -1.8 3,392,213 + -0.6 3,392,214 - -1.3 3,392,214 - +0.3 3,392,214 - -0.1 3,392,214 - -1.1 3,392,214 - -1.3 3,392,214 - +2.4 3,392,214 - +0.7 3,392,214 - -1.7 3,392,214 - +1.2 3,392,214 - -1.0 3,392,214 - +1.3 3,392,214 - +0.1 3,392,233 + -2.0 3,392,254 - +1.6 3,392,279 - +1.0 3,392,279 - -1.5 3,392,279 - +0.6 3,392,283 + +1.0 3,392,283 + -0.7 3,392,283 + -0.5 3,392,283 + +0.1 3,392,284 - +1.3 3,392,305 + -0.8 3,392,305 + -0.2 3,392,306 - +1.9 3,392,306 - -0.2 3,392,438 + Psest_3219 0.15 +1.2 3,392,438 + Psest_3219 0.15 -0.8 3,392,438 + Psest_3219 0.15 +0.4 3,392,439 - Psest_3219 0.15 -0.0 3,392,439 - Psest_3219 0.15 -0.7 3,392,440 + Psest_3219 0.15 -0.5 3,392,440 + Psest_3219 0.15 +1.0 3,392,441 - Psest_3219 0.16 -0.1 3,392,441 - Psest_3219 0.16 -0.2 3,392,441 - Psest_3219 0.16 -0.0 3,392,582 + Psest_3219 0.37 -0.3 3,392,582 + Psest_3219 0.37 -1.0 3,392,583 - Psest_3219 0.37 -1.0 3,392,583 - Psest_3219 0.37 -0.0 3,392,583 - Psest_3219 0.37 +0.9 3,392,583 - Psest_3219 0.37 +0.0 3,392,671 + Psest_3219 0.51 -1.2 3,392,672 - Psest_3219 0.51 -0.8 3,392,672 - Psest_3219 0.51 +0.8 3,392,672 - Psest_3219 0.51 -1.1 3,393,012 + -1.0 3,393,319 + +0.3 3,393,319 + -0.9 3,393,320 - +0.2 3,393,328 + +1.5 3,393,501 + +0.4 3,393,501 + +0.8 3,393,501 + -0.5 3,393,502 - -1.0 3,393,502 - -0.5 3,393,631 + Psest_3221 0.16 +0.4 3,393,631 + Psest_3221 0.16 +0.4 3,393,824 + Psest_3221 0.28 +2.6 3,393,824 + Psest_3221 0.28 -0.1 3,393,825 - Psest_3221 0.28 +3.5 3,393,825 - Psest_3221 0.28 +0.0 3,393,825 - Psest_3221 0.28 -2.0 3,393,825 - Psest_3221 0.28 +1.1 3,393,825 - Psest_3221 0.28 +1.3 3,393,825 - Psest_3221 0.28 +1.8 3,393,825 - Psest_3221 0.28 +0.7 3,393,827 + Psest_3221 0.28 +1.8 3,393,827 + Psest_3221 0.28 -0.7 3,393,828 - Psest_3221 0.28 +1.0 3,393,828 - Psest_3221 0.28 +0.7 3,393,838 - Psest_3221 0.29 +0.5 3,393,857 - Psest_3221 0.30 -0.8
Or see this region's nucleotide sequence