Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0314 and Psest_0315 are separated by 288 nucleotides Psest_0315 and Psest_0316 are separated by 107 nucleotides Psest_0316 and Psest_0317 are separated by 9 nucleotides Psest_0317 and Psest_0318 are separated by 60 nucleotides
Psest_0314: Psest_0314 - adenosylhomocysteinase, at 337,219 to 338,625
_0314
Psest_0315: Psest_0315 - hypothetical protein, at 338,914 to 339,351
_0315
Psest_0316: Psest_0316 - Cytochrome c556, at 339,459 to 339,905
_0316
Psest_0317: Psest_0317 - Protein of unknown function (DUF1090)., at 339,915 to 340,295
_0317
Psest_0318: Psest_0318 - NAD-dependent DNA ligase (contains BRCT domain type II), at 340,356 to 342,020
_0318
Position (kb) 339
340 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 338.645 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.677 kb on - strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.797 kb on + strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.798 kb on - strand at 338.840 kb on + strand at 338.840 kb on + strand at 338.840 kb on + strand at 338.840 kb on + strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.841 kb on - strand at 338.862 kb on + strand at 338.863 kb on - strand at 338.989 kb on + strand, within Psest_0315 at 338.989 kb on + strand, within Psest_0315 at 338.990 kb on - strand, within Psest_0315 at 338.990 kb on - strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.993 kb on + strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 338.994 kb on - strand, within Psest_0315 at 339.060 kb on + strand, within Psest_0315 at 339.060 kb on + strand, within Psest_0315 at 339.251 kb on + strand, within Psest_0315 at 339.251 kb on + strand, within Psest_0315 at 339.251 kb on + strand, within Psest_0315 at 339.252 kb on - strand, within Psest_0315 at 339.252 kb on - strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.277 kb on + strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.278 kb on - strand, within Psest_0315 at 339.283 kb on + strand, within Psest_0315 at 339.425 kb on - strand at 339.503 kb on - strand at 339.503 kb on - strand at 339.561 kb on + strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.916 kb on + strand at 339.917 kb on - strand at 340.032 kb on + strand, within Psest_0317 at 340.055 kb on - strand, within Psest_0317 at 340.308 kb on + strand at 340.308 kb on + strand at 340.308 kb on + strand at 340.308 kb on + strand at 340.309 kb on - strand at 340.309 kb on - strand at 340.309 kb on - strand at 340.327 kb on - strand at 340.371 kb on - strand at 340.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 338,645 - -1.9 338,647 - -2.2 338,647 - -0.2 338,647 - +2.6 338,647 - +1.0 338,647 - +0.9 338,647 - +0.9 338,647 - -1.3 338,647 - +0.3 338,647 - +1.3 338,647 - -0.7 338,677 - +1.8 338,797 + -0.7 338,797 + -0.8 338,797 + +0.8 338,797 + -0.1 338,797 + -1.2 338,797 + -0.6 338,797 + -2.6 338,797 + +0.2 338,798 - +1.0 338,798 - +0.6 338,798 - +1.9 338,798 - -0.5 338,798 - +0.7 338,798 - +1.0 338,798 - -1.3 338,840 + -0.7 338,840 + -0.7 338,840 + +1.9 338,840 + +1.8 338,841 - -1.8 338,841 - +0.5 338,841 - -0.5 338,841 - -1.2 338,841 - -0.2 338,841 - -0.4 338,841 - -0.6 338,841 - -0.7 338,841 - -0.8 338,841 - +1.3 338,862 + +1.0 338,863 - -1.0 338,989 + Psest_0315 0.17 -0.6 338,989 + Psest_0315 0.17 +3.6 338,990 - Psest_0315 0.17 -1.3 338,990 - Psest_0315 0.17 -0.1 338,993 + Psest_0315 0.18 -1.6 338,993 + Psest_0315 0.18 +1.6 338,993 + Psest_0315 0.18 -0.7 338,993 + Psest_0315 0.18 -0.1 338,993 + Psest_0315 0.18 -0.9 338,993 + Psest_0315 0.18 -1.3 338,993 + Psest_0315 0.18 -0.5 338,993 + Psest_0315 0.18 -1.7 338,993 + Psest_0315 0.18 -0.5 338,993 + Psest_0315 0.18 -1.3 338,993 + Psest_0315 0.18 +2.4 338,994 - Psest_0315 0.18 +1.3 338,994 - Psest_0315 0.18 +1.5 338,994 - Psest_0315 0.18 +0.1 338,994 - Psest_0315 0.18 +0.0 338,994 - Psest_0315 0.18 -2.5 338,994 - Psest_0315 0.18 -0.8 338,994 - Psest_0315 0.18 -0.5 338,994 - Psest_0315 0.18 -0.5 338,994 - Psest_0315 0.18 +2.5 338,994 - Psest_0315 0.18 +3.1 338,994 - Psest_0315 0.18 -1.4 338,994 - Psest_0315 0.18 -0.2 338,994 - Psest_0315 0.18 +1.6 338,994 - Psest_0315 0.18 -1.9 339,060 + Psest_0315 0.33 +0.2 339,060 + Psest_0315 0.33 -1.9 339,251 + Psest_0315 0.77 -0.8 339,251 + Psest_0315 0.77 -2.7 339,251 + Psest_0315 0.77 -1.1 339,252 - Psest_0315 0.77 +0.6 339,252 - Psest_0315 0.77 +0.2 339,277 + Psest_0315 0.83 +1.0 339,277 + Psest_0315 0.83 -2.3 339,277 + Psest_0315 0.83 +1.0 339,277 + Psest_0315 0.83 +1.2 339,277 + Psest_0315 0.83 +3.1 339,277 + Psest_0315 0.83 +0.6 339,278 - Psest_0315 0.83 +1.3 339,278 - Psest_0315 0.83 +1.6 339,278 - Psest_0315 0.83 -1.8 339,278 - Psest_0315 0.83 +2.1 339,278 - Psest_0315 0.83 +3.5 339,278 - Psest_0315 0.83 -1.8 339,278 - Psest_0315 0.83 +0.7 339,278 - Psest_0315 0.83 +0.2 339,278 - Psest_0315 0.83 -1.3 339,283 + Psest_0315 0.84 -0.8 339,425 - -0.0 339,503 - +0.0 339,503 - +3.1 339,561 + Psest_0316 0.23 -0.4 339,562 - Psest_0316 0.23 +3.4 339,562 - Psest_0316 0.23 +3.0 339,562 - Psest_0316 0.23 +2.1 339,916 + +0.3 339,917 - -1.4 340,032 + Psest_0317 0.31 +0.3 340,055 - Psest_0317 0.37 -1.6 340,308 + -0.5 340,308 + -0.1 340,308 + +3.3 340,308 + -0.2 340,309 - +1.0 340,309 - -0.9 340,309 - -2.6 340,327 - +2.7 340,371 - +1.5 340,371 - +0.3
Or see this region's nucleotide sequence