Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2453 and Psest_2454 are separated by 78 nucleotides Psest_2454 and Psest_2455 are separated by 41 nucleotides Psest_2455 and Psest_2456 are separated by 119 nucleotides
Psest_2453: Psest_2453 - Thiosulfate reductase cytochrome B subunit (membrane anchoring protein), at 2,622,467 to 2,623,063
_2453
Psest_2454: Psest_2454 - Rhodanese-related sulfurtransferase, at 2,623,142 to 2,623,690
_2454
Psest_2455: Psest_2455 - Acyl-CoA thioesterase, at 2,623,732 to 2,624,529
_2455
Psest_2456: Psest_2456 - Transglutaminase-like enzymes, putative cysteine proteases, at 2,624,649 to 2,626,649
_2456
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2622.732 kb on - strand, within Psest_2453 at 2622.735 kb on - strand, within Psest_2453 at 2622.735 kb on - strand, within Psest_2453 at 2622.818 kb on + strand, within Psest_2453 at 2622.818 kb on + strand, within Psest_2453 at 2622.819 kb on - strand, within Psest_2453 at 2622.819 kb on - strand, within Psest_2453 at 2622.819 kb on - strand, within Psest_2453 at 2622.819 kb on - strand, within Psest_2453 at 2622.819 kb on - strand, within Psest_2453 at 2622.872 kb on + strand, within Psest_2453 at 2622.872 kb on + strand, within Psest_2453 at 2622.872 kb on + strand, within Psest_2453 at 2622.873 kb on - strand, within Psest_2453 at 2622.873 kb on - strand, within Psest_2453 at 2622.944 kb on - strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.987 kb on + strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.988 kb on - strand, within Psest_2453 at 2622.989 kb on + strand, within Psest_2453 at 2622.990 kb on - strand, within Psest_2453 at 2623.083 kb on - strand at 2623.096 kb on + strand at 2623.096 kb on + strand at 2623.097 kb on - strand at 2623.098 kb on + strand at 2623.116 kb on + strand at 2623.116 kb on + strand at 2623.117 kb on - strand at 2623.229 kb on + strand, within Psest_2454 at 2623.338 kb on - strand, within Psest_2454 at 2623.547 kb on + strand, within Psest_2454 at 2623.547 kb on + strand, within Psest_2454 at 2623.671 kb on - strand at 2623.847 kb on + strand, within Psest_2455 at 2623.847 kb on + strand, within Psest_2455 at 2624.055 kb on + strand, within Psest_2455 at 2624.055 kb on + strand, within Psest_2455 at 2624.055 kb on + strand, within Psest_2455 at 2624.055 kb on + strand, within Psest_2455 at 2624.055 kb on + strand, within Psest_2455 at 2624.056 kb on - strand, within Psest_2455 at 2624.056 kb on - strand, within Psest_2455 at 2624.056 kb on - strand, within Psest_2455 at 2624.110 kb on - strand, within Psest_2455 at 2624.123 kb on - strand, within Psest_2455 at 2624.131 kb on + strand, within Psest_2455 at 2624.132 kb on - strand, within Psest_2455 at 2624.132 kb on - strand, within Psest_2455 at 2624.135 kb on + strand, within Psest_2455 at 2624.135 kb on + strand, within Psest_2455 at 2624.135 kb on + strand, within Psest_2455 at 2624.136 kb on - strand, within Psest_2455 at 2624.136 kb on - strand, within Psest_2455 at 2624.136 kb on - strand, within Psest_2455 at 2624.500 kb on + strand at 2624.552 kb on - strand at 2624.711 kb on + strand at 2624.711 kb on + strand at 2624.711 kb on + strand at 2624.712 kb on - strand at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.015 kb on + strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.016 kb on - strand, within Psest_2456 at 2625.064 kb on + strand, within Psest_2456 at 2625.065 kb on - strand, within Psest_2456 at 2625.073 kb on - strand, within Psest_2456 at 2625.091 kb on + strand, within Psest_2456 at 2625.092 kb on - strand, within Psest_2456 at 2625.092 kb on - strand, within Psest_2456 at 2625.284 kb on - strand, within Psest_2456 at 2625.325 kb on + strand, within Psest_2456 at 2625.325 kb on + strand, within Psest_2456 at 2625.325 kb on + strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.326 kb on - strand, within Psest_2456 at 2625.502 kb on + strand, within Psest_2456 at 2625.502 kb on + strand, within Psest_2456 at 2625.503 kb on - strand, within Psest_2456 at 2625.509 kb on - strand, within Psest_2456
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 2,622,732 - Psest_2453 0.44 -1.0 2,622,735 - Psest_2453 0.45 -2.1 2,622,735 - Psest_2453 0.45 -0.4 2,622,818 + Psest_2453 0.59 -2.1 2,622,818 + Psest_2453 0.59 -1.7 2,622,819 - Psest_2453 0.59 +0.1 2,622,819 - Psest_2453 0.59 +0.6 2,622,819 - Psest_2453 0.59 -0.2 2,622,819 - Psest_2453 0.59 -1.2 2,622,819 - Psest_2453 0.59 +2.0 2,622,872 + Psest_2453 0.68 +0.6 2,622,872 + Psest_2453 0.68 +2.4 2,622,872 + Psest_2453 0.68 +0.8 2,622,873 - Psest_2453 0.68 -0.2 2,622,873 - Psest_2453 0.68 +0.2 2,622,944 - Psest_2453 0.80 +1.9 2,622,987 + Psest_2453 0.87 +0.4 2,622,987 + Psest_2453 0.87 -0.2 2,622,987 + Psest_2453 0.87 -0.7 2,622,987 + Psest_2453 0.87 -0.2 2,622,987 + Psest_2453 0.87 +0.3 2,622,987 + Psest_2453 0.87 -2.3 2,622,987 + Psest_2453 0.87 +0.7 2,622,987 + Psest_2453 0.87 +2.5 2,622,987 + Psest_2453 0.87 +0.4 2,622,987 + Psest_2453 0.87 -0.4 2,622,987 + Psest_2453 0.87 -0.2 2,622,987 + Psest_2453 0.87 -1.3 2,622,988 - Psest_2453 0.87 +1.1 2,622,988 - Psest_2453 0.87 +0.3 2,622,988 - Psest_2453 0.87 -0.7 2,622,988 - Psest_2453 0.87 -1.2 2,622,988 - Psest_2453 0.87 -0.6 2,622,988 - Psest_2453 0.87 -0.1 2,622,988 - Psest_2453 0.87 -1.5 2,622,988 - Psest_2453 0.87 -0.1 2,622,988 - Psest_2453 0.87 +2.3 2,622,988 - Psest_2453 0.87 -1.0 2,622,988 - Psest_2453 0.87 -1.2 2,622,988 - Psest_2453 0.87 -1.6 2,622,988 - Psest_2453 0.87 +0.2 2,622,988 - Psest_2453 0.87 +1.7 2,622,988 - Psest_2453 0.87 +1.0 2,622,988 - Psest_2453 0.87 +0.1 2,622,988 - Psest_2453 0.87 -0.8 2,622,988 - Psest_2453 0.87 +2.4 2,622,988 - Psest_2453 0.87 +0.6 2,622,988 - Psest_2453 0.87 +1.4 2,622,988 - Psest_2453 0.87 -0.7 2,622,988 - Psest_2453 0.87 +1.2 2,622,989 + Psest_2453 0.87 -0.4 2,622,990 - Psest_2453 0.88 +0.1 2,623,083 - -2.2 2,623,096 + -0.1 2,623,096 + -1.1 2,623,097 - +1.8 2,623,098 + +1.8 2,623,116 + +2.0 2,623,116 + -2.7 2,623,117 - +0.2 2,623,229 + Psest_2454 0.16 +1.2 2,623,338 - Psest_2454 0.36 +0.0 2,623,547 + Psest_2454 0.74 -1.2 2,623,547 + Psest_2454 0.74 +0.6 2,623,671 - +0.1 2,623,847 + Psest_2455 0.14 -1.6 2,623,847 + Psest_2455 0.14 -0.6 2,624,055 + Psest_2455 0.40 -1.0 2,624,055 + Psest_2455 0.40 -2.2 2,624,055 + Psest_2455 0.40 -0.4 2,624,055 + Psest_2455 0.40 -2.7 2,624,055 + Psest_2455 0.40 -1.8 2,624,056 - Psest_2455 0.41 -0.8 2,624,056 - Psest_2455 0.41 +1.0 2,624,056 - Psest_2455 0.41 +1.8 2,624,110 - Psest_2455 0.47 -0.7 2,624,123 - Psest_2455 0.49 +0.0 2,624,131 + Psest_2455 0.50 +0.5 2,624,132 - Psest_2455 0.50 -1.6 2,624,132 - Psest_2455 0.50 -0.3 2,624,135 + Psest_2455 0.51 -2.7 2,624,135 + Psest_2455 0.51 -0.8 2,624,135 + Psest_2455 0.51 +0.8 2,624,136 - Psest_2455 0.51 -0.8 2,624,136 - Psest_2455 0.51 -0.4 2,624,136 - Psest_2455 0.51 +0.8 2,624,500 + +0.0 2,624,552 - -1.2 2,624,711 + -0.2 2,624,711 + -0.8 2,624,711 + -1.7 2,624,712 - +0.1 2,625,015 + Psest_2456 0.18 +0.7 2,625,015 + Psest_2456 0.18 -0.3 2,625,015 + Psest_2456 0.18 -0.8 2,625,015 + Psest_2456 0.18 -0.2 2,625,015 + Psest_2456 0.18 +0.3 2,625,015 + Psest_2456 0.18 +1.1 2,625,015 + Psest_2456 0.18 +1.6 2,625,016 - Psest_2456 0.18 -0.6 2,625,016 - Psest_2456 0.18 -0.5 2,625,016 - Psest_2456 0.18 +0.5 2,625,016 - Psest_2456 0.18 +0.2 2,625,016 - Psest_2456 0.18 -1.8 2,625,016 - Psest_2456 0.18 +0.1 2,625,016 - Psest_2456 0.18 +2.3 2,625,016 - Psest_2456 0.18 -0.8 2,625,064 + Psest_2456 0.21 -1.1 2,625,065 - Psest_2456 0.21 -1.1 2,625,073 - Psest_2456 0.21 -0.8 2,625,091 + Psest_2456 0.22 +1.6 2,625,092 - Psest_2456 0.22 -0.8 2,625,092 - Psest_2456 0.22 -0.0 2,625,284 - Psest_2456 0.32 -1.0 2,625,325 + Psest_2456 0.34 +0.6 2,625,325 + Psest_2456 0.34 +0.8 2,625,325 + Psest_2456 0.34 -3.3 2,625,326 - Psest_2456 0.34 -0.8 2,625,326 - Psest_2456 0.34 -0.8 2,625,326 - Psest_2456 0.34 -0.2 2,625,326 - Psest_2456 0.34 +0.2 2,625,326 - Psest_2456 0.34 -0.1 2,625,326 - Psest_2456 0.34 -0.5 2,625,326 - Psest_2456 0.34 +0.5 2,625,502 + Psest_2456 0.43 -1.0 2,625,502 + Psest_2456 0.43 +1.0 2,625,503 - Psest_2456 0.43 -0.2 2,625,509 - Psest_2456 0.43 +1.5
Or see this region's nucleotide sequence