Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0789 and Psest_0790 are separated by 68 nucleotides Psest_0790 and Psest_0791 are separated by 166 nucleotides Psest_0791 and Psest_0792 are separated by 275 nucleotides
Psest_0789: Psest_0789 - hypothetical protein, at 862,381 to 862,734
_0789
Psest_0790: Psest_0790 - Uncharacterized protein conserved in bacteria, at 862,803 to 863,243
_0790
Psest_0791: Psest_0791 - Acyl-CoA dehydrogenases, at 863,410 to 864,660
_0791
Psest_0792: Psest_0792 - Regulator of nitric oxide reductase transcription, at 864,936 to 867,113
_0792
Position (kb) 863
864
865 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 862.431 kb on + strand, within Psest_0789 at 862.432 kb on - strand, within Psest_0789 at 862.432 kb on - strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.618 kb on + strand, within Psest_0789 at 862.619 kb on - strand, within Psest_0789 at 862.625 kb on + strand, within Psest_0789 at 862.626 kb on - strand, within Psest_0789 at 862.810 kb on - strand at 862.989 kb on + strand, within Psest_0790 at 863.083 kb on + strand, within Psest_0790 at 863.083 kb on + strand, within Psest_0790 at 863.084 kb on - strand, within Psest_0790 at 863.229 kb on + strand at 863.229 kb on + strand at 863.229 kb on + strand at 863.229 kb on + strand at 863.230 kb on - strand at 863.230 kb on - strand at 863.370 kb on + strand at 863.371 kb on - strand at 863.422 kb on + strand at 863.527 kb on + strand at 863.887 kb on + strand, within Psest_0791 at 863.888 kb on - strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.277 kb on + strand, within Psest_0791 at 864.278 kb on - strand, within Psest_0791 at 864.278 kb on - strand, within Psest_0791 at 864.289 kb on + strand, within Psest_0791 at 864.475 kb on + strand, within Psest_0791 at 864.475 kb on + strand, within Psest_0791 at 864.634 kb on + strand at 864.634 kb on + strand at 864.638 kb on + strand at 864.639 kb on - strand at 864.639 kb on - strand at 864.639 kb on - strand at 864.639 kb on - strand at 864.639 kb on - strand at 864.775 kb on + strand at 864.775 kb on + strand at 864.776 kb on - strand at 864.776 kb on - strand at 864.776 kb on - strand at 864.776 kb on - strand at 864.776 kb on - strand at 864.787 kb on + strand at 864.787 kb on + strand at 864.788 kb on - strand at 864.896 kb on + strand at 864.896 kb on + strand at 864.896 kb on + strand at 864.896 kb on + strand at 864.896 kb on + strand at 864.896 kb on + strand at 864.897 kb on - strand at 864.897 kb on - strand at 864.897 kb on - strand at 864.897 kb on - strand at 864.897 kb on - strand at 864.897 kb on - strand at 864.925 kb on + strand at 864.925 kb on + strand at 864.925 kb on + strand at 864.926 kb on - strand at 864.990 kb on - strand at 865.048 kb on - strand at 865.117 kb on - strand at 865.158 kb on + strand, within Psest_0792 at 865.197 kb on + strand, within Psest_0792 at 865.198 kb on - strand, within Psest_0792 at 865.260 kb on + strand, within Psest_0792 at 865.260 kb on + strand, within Psest_0792 at 865.260 kb on + strand, within Psest_0792 at 865.260 kb on + strand, within Psest_0792 at 865.261 kb on - strand, within Psest_0792 at 865.261 kb on - strand, within Psest_0792 at 865.261 kb on - strand, within Psest_0792 at 865.261 kb on - strand, within Psest_0792 at 865.261 kb on - strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.335 kb on + strand, within Psest_0792 at 865.336 kb on - strand, within Psest_0792
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 862,431 + Psest_0789 0.14 -0.3 862,432 - Psest_0789 0.14 +0.4 862,432 - Psest_0789 0.14 -0.8 862,618 + Psest_0789 0.67 -0.4 862,618 + Psest_0789 0.67 -0.5 862,618 + Psest_0789 0.67 +0.8 862,618 + Psest_0789 0.67 -1.0 862,618 + Psest_0789 0.67 +1.7 862,618 + Psest_0789 0.67 +1.3 862,619 - Psest_0789 0.67 +0.4 862,625 + Psest_0789 0.69 +4.3 862,626 - Psest_0789 0.69 +0.2 862,810 - +2.7 862,989 + Psest_0790 0.42 -0.2 863,083 + Psest_0790 0.63 +0.2 863,083 + Psest_0790 0.63 -0.3 863,084 - Psest_0790 0.64 -0.8 863,229 + -0.6 863,229 + +1.8 863,229 + -0.2 863,229 + +1.6 863,230 - -1.0 863,230 - -1.0 863,370 + -1.6 863,371 - +1.7 863,422 + +0.4 863,527 + +0.4 863,887 + Psest_0791 0.38 +0.6 863,888 - Psest_0791 0.38 +1.9 864,277 + Psest_0791 0.69 +0.4 864,277 + Psest_0791 0.69 +0.2 864,277 + Psest_0791 0.69 +0.8 864,277 + Psest_0791 0.69 -1.3 864,277 + Psest_0791 0.69 +2.5 864,277 + Psest_0791 0.69 -0.3 864,277 + Psest_0791 0.69 -0.1 864,278 - Psest_0791 0.69 +0.6 864,278 - Psest_0791 0.69 +3.7 864,289 + Psest_0791 0.70 -0.0 864,475 + Psest_0791 0.85 +1.6 864,475 + Psest_0791 0.85 +2.3 864,634 + -0.9 864,634 + -0.5 864,638 + -1.0 864,639 - +0.4 864,639 - -0.2 864,639 - +1.7 864,639 - +2.6 864,639 - +0.6 864,775 + +0.2 864,775 + +0.7 864,776 - -0.7 864,776 - -0.9 864,776 - -0.2 864,776 - +1.9 864,776 - +2.3 864,787 + -1.7 864,787 + -0.7 864,788 - +3.0 864,896 + -1.5 864,896 + -1.3 864,896 + +0.8 864,896 + +1.3 864,896 + -1.1 864,896 + +1.6 864,897 - -0.4 864,897 - -2.6 864,897 - +1.0 864,897 - +0.1 864,897 - +2.6 864,897 - -2.9 864,925 + +0.4 864,925 + +1.2 864,925 + +0.1 864,926 - -0.7 864,990 - -3.4 865,048 - -0.4 865,117 - +1.7 865,158 + Psest_0792 0.10 -2.8 865,197 + Psest_0792 0.12 -1.2 865,198 - Psest_0792 0.12 -0.0 865,260 + Psest_0792 0.15 +0.5 865,260 + Psest_0792 0.15 -0.3 865,260 + Psest_0792 0.15 -0.1 865,260 + Psest_0792 0.15 -1.7 865,261 - Psest_0792 0.15 -2.8 865,261 - Psest_0792 0.15 -1.6 865,261 - Psest_0792 0.15 -1.8 865,261 - Psest_0792 0.15 -0.3 865,261 - Psest_0792 0.15 -0.5 865,335 + Psest_0792 0.18 +0.1 865,335 + Psest_0792 0.18 -1.0 865,335 + Psest_0792 0.18 +2.2 865,335 + Psest_0792 0.18 +0.5 865,335 + Psest_0792 0.18 -0.9 865,335 + Psest_0792 0.18 +1.6 865,335 + Psest_0792 0.18 +1.1 865,336 - Psest_0792 0.18 +0.7
Or see this region's nucleotide sequence