Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4347 and Psest_4348 overlap by 8 nucleotides Psest_4348 and Psest_4349 are separated by 61 nucleotides Psest_4349 and Psest_4350 are separated by 2 nucleotides
Psest_4347: Psest_4347 - PAS domain S-box, at 4,546,139 to 4,548,544
_4347
Psest_4348: Psest_4348 - Heme oxygenase, at 4,548,537 to 4,549,091
_4348
Psest_4349: Psest_4349 - glucosamine--fructose-6-phosphate aminotransferase (isomerizing), at 4,549,153 to 4,551,003
_4349
Psest_4350: Psest_4350 - Transcriptional regulators of sugar metabolism, at 4,551,006 to 4,551,788
_4350
Position (kb) 4549
4550
4551
4552 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4548.409 kb on - strand at 4548.422 kb on + strand at 4548.548 kb on - strand at 4548.548 kb on - strand at 4548.603 kb on + strand, within Psest_4348 at 4548.604 kb on - strand, within Psest_4348 at 4548.604 kb on - strand, within Psest_4348 at 4548.814 kb on + strand, within Psest_4348 at 4548.823 kb on + strand, within Psest_4348 at 4548.823 kb on + strand, within Psest_4348 at 4548.823 kb on + strand, within Psest_4348 at 4548.823 kb on + strand, within Psest_4348 at 4548.823 kb on + strand, within Psest_4348 at 4548.824 kb on - strand, within Psest_4348 at 4548.824 kb on - strand, within Psest_4348 at 4548.846 kb on + strand, within Psest_4348 at 4548.847 kb on - strand, within Psest_4348 at 4548.847 kb on - strand, within Psest_4348 at 4548.847 kb on - strand, within Psest_4348 at 4548.847 kb on - strand, within Psest_4348 at 4548.895 kb on + strand, within Psest_4348 at 4548.896 kb on - strand, within Psest_4348 at 4549.061 kb on + strand at 4549.062 kb on - strand at 4549.078 kb on - strand at 4549.083 kb on + strand at 4549.084 kb on - strand at 4549.084 kb on - strand at 4549.084 kb on - strand at 4549.084 kb on - strand at 4551.226 kb on - strand, within Psest_4350 at 4551.226 kb on - strand, within Psest_4350 at 4551.226 kb on - strand, within Psest_4350 at 4551.243 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 4,548,409 - -0.9 4,548,422 + -0.9 4,548,548 - -3.1 4,548,548 - +1.0 4,548,603 + Psest_4348 0.12 +1.2 4,548,604 - Psest_4348 0.12 -0.4 4,548,604 - Psest_4348 0.12 +0.2 4,548,814 + Psest_4348 0.50 -1.1 4,548,823 + Psest_4348 0.52 +1.4 4,548,823 + Psest_4348 0.52 +1.8 4,548,823 + Psest_4348 0.52 +0.9 4,548,823 + Psest_4348 0.52 -0.4 4,548,823 + Psest_4348 0.52 -0.4 4,548,824 - Psest_4348 0.52 +2.5 4,548,824 - Psest_4348 0.52 +0.3 4,548,846 + Psest_4348 0.56 +1.0 4,548,847 - Psest_4348 0.56 +2.1 4,548,847 - Psest_4348 0.56 -2.0 4,548,847 - Psest_4348 0.56 -1.9 4,548,847 - Psest_4348 0.56 -0.0 4,548,895 + Psest_4348 0.65 +0.6 4,548,896 - Psest_4348 0.65 +0.7 4,549,061 + -2.0 4,549,062 - -0.6 4,549,078 - -0.2 4,549,083 + +1.9 4,549,084 - -0.9 4,549,084 - +0.4 4,549,084 - +4.7 4,549,084 - -0.6 4,551,226 - Psest_4350 0.28 -1.9 4,551,226 - Psest_4350 0.28 -0.8 4,551,226 - Psest_4350 0.28 -2.7 4,551,243 - Psest_4350 0.30 -0.0 4,551,560 - Psest_4350 0.71 +0.2 4,551,560 - Psest_4350 0.71 -0.9 4,551,560 - Psest_4350 0.71 +0.5 4,551,576 + Psest_4350 0.73 -2.1 4,551,576 + Psest_4350 0.73 -4.1 4,551,576 + Psest_4350 0.73 -1.4 4,551,576 + Psest_4350 0.73 -2.7 4,551,576 + Psest_4350 0.73 -1.2 4,551,577 - Psest_4350 0.73 -1.7 4,551,577 - Psest_4350 0.73 -0.4 4,551,577 - Psest_4350 0.73 -0.4 4,551,793 - +0.6
Or see this region's nucleotide sequence