Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0376 and Psest_0377 are separated by 108 nucleotides Psest_0377 and Psest_0378 are separated by 93 nucleotides
Psest_0376: Psest_0376 - glucarate dehydratase, at 403,607 to 404,947
_0376
Psest_0377: Psest_0377 - galactarate dehydratase, at 405,056 to 406,621
_0377
Psest_0378: Psest_0378 - Lactate dehydrogenase and related dehydrogenases, at 406,715 to 407,644
_0378
Position (kb) 405
406
407 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 404.195 kb on + strand, within Psest_0376 at 404.195 kb on + strand, within Psest_0376 at 404.894 kb on + strand at 404.895 kb on - strand at 404.895 kb on - strand at 404.895 kb on - strand at 405.412 kb on + strand, within Psest_0377 at 405.412 kb on + strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.413 kb on - strand, within Psest_0377 at 405.623 kb on + strand, within Psest_0377 at 405.623 kb on + strand, within Psest_0377 at 405.623 kb on + strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.624 kb on - strand, within Psest_0377 at 405.962 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.037 kb on + strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.038 kb on - strand, within Psest_0377 at 406.183 kb on + strand, within Psest_0377 at 406.523 kb on + strand at 406.523 kb on + strand at 406.523 kb on + strand at 406.523 kb on + strand at 406.524 kb on - strand at 406.524 kb on - strand at 406.524 kb on - strand at 406.526 kb on + strand at 406.527 kb on - strand at 406.527 kb on - strand at 406.527 kb on - strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.722 kb on - strand at 406.722 kb on - strand at 407.154 kb on + strand, within Psest_0378 at 407.154 kb on + strand, within Psest_0378 at 407.155 kb on - strand, within Psest_0378 at 407.155 kb on - strand, within Psest_0378 at 407.219 kb on + strand, within Psest_0378 at 407.220 kb on - strand, within Psest_0378 at 407.522 kb on - strand, within Psest_0378
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 404,195 + Psest_0376 0.44 -2.3 404,195 + Psest_0376 0.44 +3.0 404,894 + +1.8 404,895 - +1.4 404,895 - -1.3 404,895 - -0.2 405,412 + Psest_0377 0.23 +1.0 405,412 + Psest_0377 0.23 -2.9 405,413 - Psest_0377 0.23 -2.6 405,413 - Psest_0377 0.23 +0.8 405,413 - Psest_0377 0.23 -1.2 405,413 - Psest_0377 0.23 -0.5 405,413 - Psest_0377 0.23 +0.8 405,413 - Psest_0377 0.23 -1.5 405,413 - Psest_0377 0.23 +1.9 405,623 + Psest_0377 0.36 +2.1 405,623 + Psest_0377 0.36 -0.2 405,623 + Psest_0377 0.36 -1.0 405,624 - Psest_0377 0.36 -0.5 405,624 - Psest_0377 0.36 -3.3 405,624 - Psest_0377 0.36 -1.4 405,624 - Psest_0377 0.36 -0.1 405,624 - Psest_0377 0.36 -1.3 405,624 - Psest_0377 0.36 +1.9 405,624 - Psest_0377 0.36 -3.3 405,962 + Psest_0377 0.58 -0.7 406,037 + Psest_0377 0.63 -0.3 406,037 + Psest_0377 0.63 +0.1 406,037 + Psest_0377 0.63 -2.9 406,037 + Psest_0377 0.63 -3.4 406,037 + Psest_0377 0.63 -0.9 406,037 + Psest_0377 0.63 +0.6 406,038 - Psest_0377 0.63 -2.3 406,038 - Psest_0377 0.63 -0.2 406,038 - Psest_0377 0.63 -0.5 406,038 - Psest_0377 0.63 -1.2 406,038 - Psest_0377 0.63 -2.9 406,038 - Psest_0377 0.63 +3.5 406,038 - Psest_0377 0.63 +1.0 406,038 - Psest_0377 0.63 -0.0 406,038 - Psest_0377 0.63 -1.2 406,038 - Psest_0377 0.63 +2.1 406,038 - Psest_0377 0.63 +0.7 406,183 + Psest_0377 0.72 -0.4 406,523 + +0.5 406,523 + -0.7 406,523 + +0.3 406,523 + -0.1 406,524 - +0.7 406,524 - -2.7 406,524 - -0.7 406,526 + +0.3 406,527 - +0.4 406,527 - +0.0 406,527 - -0.4 406,688 + -0.2 406,688 + +1.0 406,722 - -1.2 406,722 - -2.0 407,154 + Psest_0378 0.47 +0.8 407,154 + Psest_0378 0.47 +1.4 407,155 - Psest_0378 0.47 +0.1 407,155 - Psest_0378 0.47 +0.4 407,219 + Psest_0378 0.54 +2.8 407,220 - Psest_0378 0.54 -1.1 407,522 - Psest_0378 0.87 +1.0
Or see this region's nucleotide sequence