Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3085 and Psest_3086 are separated by 12 nucleotides Psest_3086 and Psest_3087 are separated by 134 nucleotides Psest_3087 and Psest_3088 overlap by 16 nucleotides Psest_3088 and Psest_3089 are separated by 127 nucleotides
Psest_3085: Psest_3085 - methyltransferase, putative, at 3,265,932 to 3,266,894
_3085
Psest_3086: Psest_3086 - hypothetical protein, at 3,266,907 to 3,267,182
_3086
Psest_3087: Psest_3087 - Transcriptional regulator, at 3,267,317 to 3,268,273
_3087
Psest_3088: Psest_3088 - Protein of unknown function (DUF2905)., at 3,268,258 to 3,268,464
_3088
Psest_3089: Psest_3089 - pyridoxine 5'-phosphate synthase, at 3,268,592 to 3,269,338
_3089
Position (kb)
3267
3268
3269 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3266.326 kb on - strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.328 kb on + strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.329 kb on - strand, within Psest_3085 at 3266.520 kb on + strand, within Psest_3085 at 3266.893 kb on - strand at 3267.093 kb on - strand, within Psest_3086 at 3267.117 kb on - strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.118 kb on + strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.119 kb on - strand, within Psest_3086 at 3267.280 kb on + strand at 3267.280 kb on + strand at 3267.280 kb on + strand at 3267.281 kb on - strand at 3267.437 kb on + strand, within Psest_3087 at 3267.437 kb on + strand, within Psest_3087 at 3267.532 kb on + strand, within Psest_3087 at 3267.661 kb on + strand, within Psest_3087 at 3267.667 kb on + strand, within Psest_3087 at 3267.837 kb on + strand, within Psest_3087 at 3267.837 kb on + strand, within Psest_3087 at 3267.838 kb on - strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.841 kb on + strand, within Psest_3087 at 3267.983 kb on - strand, within Psest_3087 at 3268.038 kb on + strand, within Psest_3087 at 3268.293 kb on - strand, within Psest_3088 at 3268.318 kb on + strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.319 kb on - strand, within Psest_3088 at 3268.475 kb on - strand at 3268.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 3,266,326 - Psest_3085 0.41 +0.5 3,266,328 + Psest_3085 0.41 +1.4 3,266,328 + Psest_3085 0.41 -0.0 3,266,328 + Psest_3085 0.41 -2.0 3,266,328 + Psest_3085 0.41 -0.2 3,266,328 + Psest_3085 0.41 +1.6 3,266,328 + Psest_3085 0.41 -0.5 3,266,328 + Psest_3085 0.41 -1.0 3,266,328 + Psest_3085 0.41 -0.3 3,266,328 + Psest_3085 0.41 -2.9 3,266,328 + Psest_3085 0.41 -0.3 3,266,328 + Psest_3085 0.41 +0.9 3,266,328 + Psest_3085 0.41 -0.4 3,266,328 + Psest_3085 0.41 +1.2 3,266,328 + Psest_3085 0.41 -0.6 3,266,328 + Psest_3085 0.41 +0.2 3,266,329 - Psest_3085 0.41 +0.6 3,266,329 - Psest_3085 0.41 +1.0 3,266,329 - Psest_3085 0.41 -1.9 3,266,329 - Psest_3085 0.41 +0.8 3,266,329 - Psest_3085 0.41 +2.9 3,266,329 - Psest_3085 0.41 +2.5 3,266,329 - Psest_3085 0.41 -1.4 3,266,329 - Psest_3085 0.41 -0.7 3,266,329 - Psest_3085 0.41 -1.7 3,266,329 - Psest_3085 0.41 -0.1 3,266,329 - Psest_3085 0.41 -1.1 3,266,329 - Psest_3085 0.41 +0.5 3,266,329 - Psest_3085 0.41 +0.8 3,266,520 + Psest_3085 0.61 +0.5 3,266,893 - +0.6 3,267,093 - Psest_3086 0.67 -1.6 3,267,117 - Psest_3086 0.76 +0.3 3,267,118 + Psest_3086 0.76 -0.3 3,267,118 + Psest_3086 0.76 +1.1 3,267,118 + Psest_3086 0.76 +1.9 3,267,118 + Psest_3086 0.76 -0.1 3,267,118 + Psest_3086 0.76 -0.1 3,267,118 + Psest_3086 0.76 +2.6 3,267,118 + Psest_3086 0.76 -0.1 3,267,119 - Psest_3086 0.77 -0.6 3,267,119 - Psest_3086 0.77 +4.6 3,267,119 - Psest_3086 0.77 -0.0 3,267,119 - Psest_3086 0.77 +0.5 3,267,119 - Psest_3086 0.77 +2.0 3,267,119 - Psest_3086 0.77 +0.3 3,267,119 - Psest_3086 0.77 +1.4 3,267,280 + +1.9 3,267,280 + -1.5 3,267,280 + +0.1 3,267,281 - -0.5 3,267,437 + Psest_3087 0.13 -0.1 3,267,437 + Psest_3087 0.13 +1.8 3,267,532 + Psest_3087 0.22 -0.8 3,267,661 + Psest_3087 0.36 -0.8 3,267,667 + Psest_3087 0.37 +0.2 3,267,837 + Psest_3087 0.54 -0.2 3,267,837 + Psest_3087 0.54 +1.6 3,267,838 - Psest_3087 0.54 -3.2 3,267,841 + Psest_3087 0.55 -0.1 3,267,841 + Psest_3087 0.55 +1.5 3,267,841 + Psest_3087 0.55 -0.4 3,267,983 - Psest_3087 0.70 +0.1 3,268,038 + Psest_3087 0.75 +3.6 3,268,293 - Psest_3088 0.17 +1.6 3,268,318 + Psest_3088 0.29 +0.1 3,268,319 - Psest_3088 0.29 +0.4 3,268,319 - Psest_3088 0.29 -0.3 3,268,319 - Psest_3088 0.29 -3.7 3,268,319 - Psest_3088 0.29 +0.6 3,268,475 - -1.1 3,268,564 - -1.9
Or see this region's nucleotide sequence