Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0216 and Psest_0217 are separated by 166 nucleotides Psest_0217 and Psest_0218 are separated by 9 nucleotides Psest_0218 and Psest_0219 are separated by 223 nucleotides
Psest_0216: Psest_0216 - Predicted permeases, at 222,666 to 223,574
_0216
Psest_0217: Psest_0217 - diguanylate cyclase (GGDEF) domain, at 223,741 to 224,955
_0217
Psest_0218: Psest_0218 - Acyl-CoA hydrolase, at 224,965 to 225,366
_0218
Psest_0219: Psest_0219 - phosphate binding protein, at 225,590 to 226,558
_0219
Position (kb)
223
224
225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 222.781 kb on + strand, within Psest_0216 at 222.781 kb on + strand at 222.781 kb on + strand, within Psest_0216 at 222.782 kb on - strand, within Psest_0216 at 222.937 kb on - strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.256 kb on - strand, within Psest_0216 at 223.256 kb on - strand, within Psest_0216 at 223.424 kb on + strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.519 kb on + strand at 223.588 kb on + strand at 223.589 kb on - strand at 223.589 kb on - strand at 223.589 kb on - strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.749 kb on + strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.750 kb on - strand at 223.759 kb on + strand at 223.759 kb on + strand at 223.933 kb on + strand, within Psest_0217 at 223.940 kb on - strand, within Psest_0217 at 224.050 kb on + strand, within Psest_0217 at 224.050 kb on + strand, within Psest_0217 at 224.050 kb on + strand, within Psest_0217 at 224.051 kb on - strand, within Psest_0217 at 224.051 kb on - strand, within Psest_0217 at 224.094 kb on - strand, within Psest_0217 at 224.150 kb on + strand, within Psest_0217 at 224.151 kb on - strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.332 kb on + strand, within Psest_0217 at 224.333 kb on - strand, within Psest_0217 at 224.333 kb on - strand, within Psest_0217 at 224.333 kb on - strand, within Psest_0217 at 224.333 kb on - strand, within Psest_0217 at 224.333 kb on - strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.335 kb on + strand, within Psest_0217 at 224.336 kb on - strand, within Psest_0217 at 224.336 kb on - strand, within Psest_0217 at 224.372 kb on - strand, within Psest_0217 at 224.372 kb on - strand, within Psest_0217 at 224.384 kb on - strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.566 kb on + strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.567 kb on - strand, within Psest_0217 at 224.698 kb on + strand, within Psest_0217 at 224.698 kb on + strand, within Psest_0217 at 224.698 kb on + strand, within Psest_0217 at 224.698 kb on + strand, within Psest_0217 at 224.698 kb on + strand, within Psest_0217 at 224.699 kb on - strand, within Psest_0217 at 224.699 kb on - strand, within Psest_0217 at 224.699 kb on - strand, within Psest_0217 at 224.699 kb on - strand, within Psest_0217 at 224.762 kb on + strand, within Psest_0217 at 224.762 kb on + strand, within Psest_0217 at 224.763 kb on - strand, within Psest_0217 at 224.763 kb on - strand, within Psest_0217 at 224.766 kb on + strand, within Psest_0217 at 224.766 kb on + strand, within Psest_0217 at 224.766 kb on + strand, within Psest_0217 at 224.767 kb on - strand, within Psest_0217 at 224.854 kb on + strand at 224.879 kb on - strand at 225.128 kb on + strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.129 kb on - strand, within Psest_0218 at 225.269 kb on + strand, within Psest_0218 at 225.269 kb on + strand, within Psest_0218 at 225.417 kb on - strand at 225.417 kb on - strand at 225.438 kb on + strand at 225.439 kb on - strand at 225.439 kb on - strand at 225.516 kb on + strand at 225.516 kb on + strand at 225.516 kb on + strand at 225.516 kb on + strand at 225.516 kb on + strand at 225.516 kb on + strand at 225.517 kb on - strand at 225.517 kb on - strand at 225.517 kb on - strand at 225.517 kb on - strand at 225.528 kb on - strand at 225.540 kb on + strand at 225.578 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 222,781 + Psest_0216 0.13 -1.4 222,781 + +0.2 222,781 + Psest_0216 0.13 -2.5 222,782 - Psest_0216 0.13 +1.1 222,937 - Psest_0216 0.30 -0.3 223,255 + Psest_0216 0.65 -1.4 223,255 + Psest_0216 0.65 +4.3 223,255 + Psest_0216 0.65 -0.3 223,255 + Psest_0216 0.65 +0.2 223,256 - Psest_0216 0.65 +2.3 223,256 - Psest_0216 0.65 +0.3 223,424 + Psest_0216 0.83 -2.0 223,457 - Psest_0216 0.87 +1.0 223,457 - Psest_0216 0.87 +3.5 223,457 - Psest_0216 0.87 -0.1 223,457 - Psest_0216 0.87 +2.2 223,457 - Psest_0216 0.87 +1.1 223,519 + -0.3 223,588 + +0.5 223,589 - -3.3 223,589 - +1.0 223,589 - -2.2 223,656 + -0.1 223,656 + +1.4 223,657 - -0.8 223,657 - -2.0 223,657 - -1.4 223,657 - -0.8 223,657 - -0.1 223,749 + +0.2 223,749 + +0.8 223,749 + +0.6 223,749 + -1.4 223,749 + -1.4 223,749 + +0.8 223,749 + +0.7 223,749 + +0.8 223,749 + +2.6 223,749 + +2.4 223,750 - +1.6 223,750 - -0.4 223,750 - +1.4 223,750 - -3.5 223,750 - +0.4 223,750 - -0.3 223,750 - +0.4 223,750 - +0.6 223,759 + +0.6 223,759 + +2.4 223,933 + Psest_0217 0.16 +5.4 223,940 - Psest_0217 0.16 -2.2 224,050 + Psest_0217 0.25 -0.9 224,050 + Psest_0217 0.25 +0.4 224,050 + Psest_0217 0.25 +1.7 224,051 - Psest_0217 0.26 -2.3 224,051 - Psest_0217 0.26 -1.1 224,094 - Psest_0217 0.29 -0.3 224,150 + Psest_0217 0.34 +1.0 224,151 - Psest_0217 0.34 +1.1 224,332 + Psest_0217 0.49 -2.3 224,332 + Psest_0217 0.49 +1.8 224,332 + Psest_0217 0.49 +2.3 224,332 + Psest_0217 0.49 +1.0 224,332 + Psest_0217 0.49 +4.0 224,332 + Psest_0217 0.49 +1.4 224,332 + Psest_0217 0.49 -0.1 224,332 + Psest_0217 0.49 +2.7 224,333 - Psest_0217 0.49 -0.5 224,333 - Psest_0217 0.49 +4.2 224,333 - Psest_0217 0.49 -1.3 224,333 - Psest_0217 0.49 -0.3 224,333 - Psest_0217 0.49 +0.5 224,335 + Psest_0217 0.49 +1.6 224,335 + Psest_0217 0.49 +2.4 224,335 + Psest_0217 0.49 -1.1 224,335 + Psest_0217 0.49 +0.7 224,335 + Psest_0217 0.49 -1.8 224,335 + Psest_0217 0.49 +0.2 224,335 + Psest_0217 0.49 +1.6 224,336 - Psest_0217 0.49 +0.5 224,336 - Psest_0217 0.49 -0.2 224,372 - Psest_0217 0.52 -2.1 224,372 - Psest_0217 0.52 +0.2 224,384 - Psest_0217 0.53 +1.5 224,566 + Psest_0217 0.68 -2.4 224,566 + Psest_0217 0.68 -1.0 224,566 + Psest_0217 0.68 +1.9 224,566 + Psest_0217 0.68 -1.4 224,566 + Psest_0217 0.68 -2.1 224,566 + Psest_0217 0.68 +0.7 224,567 - Psest_0217 0.68 +0.7 224,567 - Psest_0217 0.68 -1.2 224,567 - Psest_0217 0.68 +0.1 224,567 - Psest_0217 0.68 +2.6 224,567 - Psest_0217 0.68 -1.1 224,567 - Psest_0217 0.68 +0.0 224,567 - Psest_0217 0.68 -0.4 224,698 + Psest_0217 0.79 +0.8 224,698 + Psest_0217 0.79 -0.3 224,698 + Psest_0217 0.79 -1.2 224,698 + Psest_0217 0.79 -1.3 224,698 + Psest_0217 0.79 +3.8 224,699 - Psest_0217 0.79 +0.1 224,699 - Psest_0217 0.79 -0.4 224,699 - Psest_0217 0.79 -0.1 224,699 - Psest_0217 0.79 -1.5 224,762 + Psest_0217 0.84 -0.3 224,762 + Psest_0217 0.84 +1.7 224,763 - Psest_0217 0.84 -0.9 224,763 - Psest_0217 0.84 -0.4 224,766 + Psest_0217 0.84 -0.6 224,766 + Psest_0217 0.84 +0.6 224,766 + Psest_0217 0.84 -1.4 224,767 - Psest_0217 0.84 -0.4 224,854 + -1.9 224,879 - -1.2 225,128 + Psest_0218 0.41 +3.2 225,129 - Psest_0218 0.41 -0.7 225,129 - Psest_0218 0.41 -1.8 225,129 - Psest_0218 0.41 +2.1 225,129 - Psest_0218 0.41 +0.4 225,129 - Psest_0218 0.41 +1.1 225,129 - Psest_0218 0.41 +2.8 225,269 + Psest_0218 0.76 -2.3 225,269 + Psest_0218 0.76 +1.2 225,417 - -0.7 225,417 - -0.5 225,438 + -1.6 225,439 - -1.1 225,439 - -0.6 225,516 + -0.1 225,516 + +2.1 225,516 + -0.0 225,516 + -1.1 225,516 + +0.8 225,516 + -2.3 225,517 - -0.0 225,517 - -1.2 225,517 - -0.4 225,517 - +0.2 225,528 - +0.5 225,540 + +0.2 225,578 + -0.2
Or see this region's nucleotide sequence