Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0199 and Psest_0200 are separated by 281 nucleotides Psest_0200 and Psest_0201 are separated by 120 nucleotides Psest_0201 and Psest_0202 are separated by 131 nucleotides
Psest_0199: Psest_0199 - PAS domain S-box, at 197,027 to 198,613
_0199
Psest_0200: Psest_0200 - hypothetical protein, at 198,895 to 199,059
_0200
Psest_0201: Psest_0201 - PAS domain S-box, at 199,180 to 200,760
_0201
Psest_0202: Psest_0202 - diguanylate cyclase (GGDEF) domain, at 200,892 to 202,823
_0202
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 198.213 kb on + strand, within Psest_0199 at 198.300 kb on + strand, within Psest_0199 at 198.300 kb on + strand, within Psest_0199 at 198.300 kb on + strand, within Psest_0199 at 198.300 kb on + strand, within Psest_0199 at 198.301 kb on - strand, within Psest_0199 at 198.301 kb on - strand, within Psest_0199 at 198.301 kb on - strand, within Psest_0199 at 198.301 kb on - strand, within Psest_0199 at 198.331 kb on + strand, within Psest_0199 at 198.332 kb on - strand, within Psest_0199 at 198.576 kb on + strand at 198.653 kb on + strand at 198.660 kb on + strand at 198.699 kb on - strand at 198.765 kb on + strand at 198.765 kb on + strand at 198.765 kb on + strand at 198.765 kb on + strand at 198.769 kb on + strand at 198.769 kb on + strand at 198.769 kb on + strand at 198.769 kb on + strand at 198.769 kb on + strand at 198.769 kb on + strand at 198.770 kb on - strand at 198.770 kb on - strand at 198.770 kb on - strand at 198.770 kb on - strand at 198.770 kb on - strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.801 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.875 kb on + strand at 198.876 kb on - strand at 198.876 kb on - strand at 198.876 kb on - strand at 198.876 kb on - strand at 198.876 kb on - strand at 199.083 kb on + strand at 199.122 kb on - strand at 199.179 kb on + strand at 199.179 kb on + strand at 199.180 kb on - strand at 199.180 kb on - strand at 199.180 kb on - strand at 199.180 kb on - strand at 199.236 kb on + strand at 199.274 kb on - strand at 199.274 kb on - strand at 199.274 kb on - strand at 199.510 kb on + strand, within Psest_0201 at 199.511 kb on - strand, within Psest_0201 at 199.511 kb on - strand, within Psest_0201 at 199.568 kb on - strand, within Psest_0201 at 199.769 kb on + strand, within Psest_0201 at 199.834 kb on + strand, within Psest_0201 at 199.834 kb on + strand, within Psest_0201 at 199.834 kb on + strand, within Psest_0201 at 199.834 kb on + strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.835 kb on - strand, within Psest_0201 at 199.837 kb on - strand, within Psest_0201 at 200.181 kb on + strand, within Psest_0201 at 200.182 kb on - strand, within Psest_0201 at 200.182 kb on - strand, within Psest_0201 at 200.243 kb on + strand, within Psest_0201 at 200.395 kb on - strand, within Psest_0201 at 200.640 kb on + strand at 200.829 kb on + strand at 200.829 kb on + strand at 200.829 kb on + strand at 200.829 kb on + strand at 200.829 kb on + strand at 200.833 kb on + strand at 200.833 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.024 kb on + strand at 201.025 kb on - strand at 201.147 kb on + strand, within Psest_0202 at 201.147 kb on + strand, within Psest_0202 at 201.147 kb on + strand, within Psest_0202 at 201.148 kb on - strand, within Psest_0202 at 201.148 kb on - strand, within Psest_0202 at 201.148 kb on - strand, within Psest_0202 at 201.178 kb on - strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.357 kb on + strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.358 kb on - strand, within Psest_0202 at 201.360 kb on - strand, within Psest_0202 at 201.360 kb on - strand, within Psest_0202 at 201.492 kb on + strand, within Psest_0202 at 201.531 kb on + strand, within Psest_0202 at 201.532 kb on - strand, within Psest_0202 at 201.532 kb on - strand, within Psest_0202 at 201.549 kb on + strand, within Psest_0202 at 201.549 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.570 kb on + strand, within Psest_0202 at 201.571 kb on - strand, within Psest_0202 at 201.571 kb on - strand, within Psest_0202 at 201.571 kb on - strand, within Psest_0202 at 201.571 kb on - strand, within Psest_0202 at 201.603 kb on + strand, within Psest_0202 at 201.603 kb on + strand, within Psest_0202 at 201.603 kb on + strand, within Psest_0202 at 201.603 kb on + strand, within Psest_0202 at 201.756 kb on + strand, within Psest_0202 at 201.757 kb on - strand, within Psest_0202
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 198,213 + Psest_0199 0.75 +0.9 198,300 + Psest_0199 0.80 +0.9 198,300 + Psest_0199 0.80 +1.0 198,300 + Psest_0199 0.80 +0.3 198,300 + Psest_0199 0.80 +2.6 198,301 - Psest_0199 0.80 -1.2 198,301 - Psest_0199 0.80 -0.8 198,301 - Psest_0199 0.80 -0.3 198,301 - Psest_0199 0.80 +1.4 198,331 + Psest_0199 0.82 +0.4 198,332 - Psest_0199 0.82 -2.3 198,576 + -1.6 198,653 + -0.2 198,660 + +1.9 198,699 - +0.8 198,765 + +0.4 198,765 + -0.5 198,765 + +0.5 198,765 + -1.1 198,769 + +0.2 198,769 + -0.4 198,769 + -0.8 198,769 + -1.1 198,769 + -0.5 198,769 + -1.6 198,770 - +0.3 198,770 - +0.2 198,770 - +0.3 198,770 - +2.1 198,770 - +0.6 198,801 + +0.9 198,801 + +0.1 198,801 + -1.5 198,801 + -1.5 198,801 + -0.8 198,801 + -1.0 198,801 + -1.8 198,801 + +0.1 198,801 + +1.9 198,801 + +0.3 198,875 + -1.0 198,875 + +1.3 198,875 + -0.9 198,875 + +0.3 198,875 + +0.5 198,875 + +2.4 198,875 + -1.0 198,876 - -1.8 198,876 - -0.2 198,876 - -0.8 198,876 - +2.5 198,876 - -0.6 199,083 + +1.9 199,122 - -2.0 199,179 + +1.2 199,179 + +0.9 199,180 - +0.4 199,180 - -1.4 199,180 - -1.3 199,180 - +1.0 199,236 + +0.8 199,274 - -1.7 199,274 - +0.0 199,274 - -2.3 199,510 + Psest_0201 0.21 -0.8 199,511 - Psest_0201 0.21 -1.0 199,511 - Psest_0201 0.21 -1.4 199,568 - Psest_0201 0.25 -1.0 199,769 + Psest_0201 0.37 -0.9 199,834 + Psest_0201 0.41 +1.4 199,834 + Psest_0201 0.41 -1.2 199,834 + Psest_0201 0.41 -1.4 199,834 + Psest_0201 0.41 -2.2 199,835 - Psest_0201 0.41 -1.4 199,835 - Psest_0201 0.41 -0.3 199,835 - Psest_0201 0.41 -1.1 199,835 - Psest_0201 0.41 -1.5 199,835 - Psest_0201 0.41 -0.4 199,835 - Psest_0201 0.41 -3.3 199,835 - Psest_0201 0.41 -0.9 199,835 - Psest_0201 0.41 -0.2 199,835 - Psest_0201 0.41 -1.4 199,837 - Psest_0201 0.42 +0.3 200,181 + Psest_0201 0.63 -1.4 200,182 - Psest_0201 0.63 +1.4 200,182 - Psest_0201 0.63 -2.0 200,243 + Psest_0201 0.67 -0.6 200,395 - Psest_0201 0.77 +1.2 200,640 + -2.8 200,829 + -0.9 200,829 + -1.5 200,829 + -0.8 200,829 + +0.7 200,829 + -1.8 200,833 + -0.1 200,833 + +3.9 201,024 + +2.1 201,024 + +1.2 201,024 + -2.1 201,024 + +2.0 201,024 + -0.6 201,024 + -0.2 201,024 + +0.8 201,024 + -0.5 201,025 - -0.9 201,147 + Psest_0202 0.13 +0.6 201,147 + Psest_0202 0.13 -1.9 201,147 + Psest_0202 0.13 +1.1 201,148 - Psest_0202 0.13 +1.2 201,148 - Psest_0202 0.13 +2.4 201,148 - Psest_0202 0.13 -0.2 201,178 - Psest_0202 0.15 +0.2 201,357 + Psest_0202 0.24 -1.3 201,357 + Psest_0202 0.24 -2.7 201,357 + Psest_0202 0.24 -0.3 201,357 + Psest_0202 0.24 -0.5 201,357 + Psest_0202 0.24 -1.2 201,357 + Psest_0202 0.24 +0.6 201,357 + Psest_0202 0.24 +1.4 201,357 + Psest_0202 0.24 -0.5 201,357 + Psest_0202 0.24 +0.0 201,358 - Psest_0202 0.24 -1.1 201,358 - Psest_0202 0.24 +0.2 201,358 - Psest_0202 0.24 -0.9 201,358 - Psest_0202 0.24 +1.7 201,358 - Psest_0202 0.24 +0.4 201,358 - Psest_0202 0.24 -3.5 201,358 - Psest_0202 0.24 +2.7 201,358 - Psest_0202 0.24 -0.6 201,358 - Psest_0202 0.24 -0.7 201,358 - Psest_0202 0.24 -1.2 201,358 - Psest_0202 0.24 -1.8 201,358 - Psest_0202 0.24 -1.6 201,358 - Psest_0202 0.24 -2.5 201,358 - Psest_0202 0.24 +0.7 201,358 - Psest_0202 0.24 -1.2 201,358 - Psest_0202 0.24 +0.1 201,358 - Psest_0202 0.24 -0.4 201,358 - Psest_0202 0.24 +0.6 201,360 - Psest_0202 0.24 -0.4 201,360 - Psest_0202 0.24 -0.3 201,492 + Psest_0202 0.31 +1.0 201,531 + Psest_0202 0.33 -1.6 201,532 - Psest_0202 0.33 -0.7 201,532 - Psest_0202 0.33 -0.5 201,549 + Psest_0202 0.34 +0.8 201,549 + Psest_0202 0.34 -3.2 201,570 + Psest_0202 0.35 -2.5 201,570 + Psest_0202 0.35 +0.1 201,570 + Psest_0202 0.35 -0.8 201,570 + Psest_0202 0.35 -3.7 201,570 + Psest_0202 0.35 +0.5 201,570 + Psest_0202 0.35 -0.9 201,570 + Psest_0202 0.35 -0.5 201,571 - Psest_0202 0.35 +0.3 201,571 - Psest_0202 0.35 -0.9 201,571 - Psest_0202 0.35 +0.8 201,571 - Psest_0202 0.35 +0.6 201,603 + Psest_0202 0.37 +1.1 201,603 + Psest_0202 0.37 +0.3 201,603 + Psest_0202 0.37 -0.0 201,603 + Psest_0202 0.37 +1.9 201,756 + Psest_0202 0.45 -1.1 201,757 - Psest_0202 0.45 +2.0
Or see this region's nucleotide sequence