Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1962 and Psest_1963 are separated by 73 nucleotides Psest_1963 and Psest_1964 are separated by 206 nucleotides Psest_1964 and Psest_1965 overlap by 4 nucleotides Psest_1965 and Psest_1966 are separated by 3 nucleotides
Psest_1962: Psest_1962 - Fucose 4-O-acetylase and related acetyltransferases, at 2,076,927 to 2,077,955
_1962
Psest_1963: Psest_1963 - Peptidase inhibitor I78 family., at 2,078,029 to 2,078,337
_1963
Psest_1964: Psest_1964 - hypothetical protein, at 2,078,544 to 2,078,945
_1964
Psest_1965: Psest_1965 - ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double-glycine peptidase domain, at 2,078,942 to 2,079,895
_1965
Psest_1966: Psest_1966 - Glycine/D-amino acid oxidases (deaminating), at 2,079,899 to 2,081,182
_1966
Position (kb)
2078
2079
2080 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2078.050 kb on + strand at 2078.088 kb on + strand, within Psest_1963 at 2078.197 kb on + strand, within Psest_1963 at 2078.290 kb on + strand, within Psest_1963 at 2078.290 kb on + strand, within Psest_1963 at 2078.291 kb on - strand, within Psest_1963 at 2078.291 kb on - strand, within Psest_1963 at 2078.349 kb on - strand at 2078.375 kb on + strand at 2078.375 kb on + strand at 2078.375 kb on + strand at 2078.376 kb on - strand at 2078.376 kb on - strand at 2078.376 kb on - strand at 2078.378 kb on + strand at 2078.455 kb on + strand at 2078.456 kb on - strand at 2078.456 kb on - strand at 2078.543 kb on + strand at 2078.598 kb on - strand, within Psest_1964 at 2078.598 kb on - strand, within Psest_1964 at 2078.598 kb on - strand, within Psest_1964 at 2078.608 kb on + strand, within Psest_1964 at 2078.609 kb on - strand, within Psest_1964 at 2078.609 kb on - strand, within Psest_1964 at 2078.702 kb on + strand, within Psest_1964 at 2078.702 kb on + strand, within Psest_1964 at 2078.702 kb on + strand, within Psest_1964 at 2078.703 kb on - strand, within Psest_1964 at 2078.703 kb on - strand, within Psest_1964 at 2078.703 kb on - strand, within Psest_1964 at 2078.703 kb on - strand, within Psest_1964 at 2078.703 kb on - strand, within Psest_1964 at 2078.775 kb on - strand, within Psest_1964 at 2078.863 kb on + strand, within Psest_1964 at 2078.961 kb on + strand at 2078.961 kb on + strand at 2078.961 kb on + strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2079.007 kb on + strand at 2079.065 kb on + strand, within Psest_1965 at 2079.066 kb on - strand, within Psest_1965 at 2079.080 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.165 kb on - strand, within Psest_1965 at 2079.165 kb on - strand, within Psest_1965 at 2079.195 kb on + strand, within Psest_1965 at 2079.195 kb on + strand, within Psest_1965 at 2079.237 kb on - strand, within Psest_1965 at 2079.400 kb on + strand, within Psest_1965 at 2079.742 kb on - strand, within Psest_1965 at 2079.927 kb on + strand at 2079.927 kb on + strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.930 kb on + strand at 2079.931 kb on - strand at 2079.931 kb on - strand at 2079.931 kb on - strand at 2079.988 kb on - strand at 2080.069 kb on + strand, within Psest_1966 at 2080.070 kb on - strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.105 kb on - strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.112 kb on - strand, within Psest_1966 at 2080.179 kb on + strand, within Psest_1966 at 2080.179 kb on + strand, within Psest_1966 at 2080.180 kb on - strand, within Psest_1966 at 2080.216 kb on + strand, within Psest_1966 at 2080.269 kb on + strand, within Psest_1966 at 2080.270 kb on - strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.288 kb on - strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.332 kb on + strand, within Psest_1966 at 2080.468 kb on - strand, within Psest_1966 at 2080.468 kb on - strand, within Psest_1966 at 2080.519 kb on + strand, within Psest_1966 at 2080.520 kb on - strand, within Psest_1966 at 2080.697 kb on + strand, within Psest_1966 at 2080.705 kb on + strand, within Psest_1966 at 2080.706 kb on - strand, within Psest_1966 at 2080.802 kb on + strand, within Psest_1966 at 2080.802 kb on + strand, within Psest_1966 at 2080.803 kb on - strand, within Psest_1966 at 2080.831 kb on + strand, within Psest_1966 at 2080.836 kb on + strand, within Psest_1966 at 2080.837 kb on - strand, within Psest_1966
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 2,078,050 + -1.3 2,078,088 + Psest_1963 0.19 +0.5 2,078,197 + Psest_1963 0.54 +1.7 2,078,290 + Psest_1963 0.84 +0.3 2,078,290 + Psest_1963 0.84 -2.0 2,078,291 - Psest_1963 0.85 +0.7 2,078,291 - Psest_1963 0.85 +1.2 2,078,349 - -1.2 2,078,375 + -1.2 2,078,375 + +0.2 2,078,375 + +2.3 2,078,376 - -0.7 2,078,376 - +1.0 2,078,376 - -0.3 2,078,378 + -0.8 2,078,455 + -2.2 2,078,456 - -0.3 2,078,456 - -1.9 2,078,543 + -1.5 2,078,598 - Psest_1964 0.13 -0.3 2,078,598 - Psest_1964 0.13 +2.7 2,078,598 - Psest_1964 0.13 +0.3 2,078,608 + Psest_1964 0.16 -1.1 2,078,609 - Psest_1964 0.16 -0.8 2,078,609 - Psest_1964 0.16 +0.9 2,078,702 + Psest_1964 0.39 +0.1 2,078,702 + Psest_1964 0.39 -1.8 2,078,702 + Psest_1964 0.39 +0.5 2,078,703 - Psest_1964 0.40 +4.0 2,078,703 - Psest_1964 0.40 +0.0 2,078,703 - Psest_1964 0.40 -1.2 2,078,703 - Psest_1964 0.40 -0.3 2,078,703 - Psest_1964 0.40 +0.3 2,078,775 - Psest_1964 0.57 +1.0 2,078,863 + Psest_1964 0.79 -0.5 2,078,961 + -0.9 2,078,961 + -0.4 2,078,961 + +0.0 2,078,962 - +0.8 2,078,962 - -1.7 2,078,962 - -1.1 2,078,962 - +0.5 2,078,962 - +1.1 2,078,962 - +1.7 2,078,962 - -2.8 2,078,962 - +1.2 2,078,962 - -1.3 2,079,007 + +0.8 2,079,065 + Psest_1965 0.13 -0.7 2,079,066 - Psest_1965 0.13 -0.0 2,079,080 + Psest_1965 0.14 -1.6 2,079,164 + Psest_1965 0.23 +0.2 2,079,164 + Psest_1965 0.23 +0.8 2,079,164 + Psest_1965 0.23 -1.8 2,079,164 + Psest_1965 0.23 -2.0 2,079,164 + Psest_1965 0.23 +0.2 2,079,165 - Psest_1965 0.23 +0.1 2,079,165 - Psest_1965 0.23 -0.9 2,079,195 + Psest_1965 0.27 +0.1 2,079,195 + Psest_1965 0.27 +0.1 2,079,237 - Psest_1965 0.31 -0.4 2,079,400 + Psest_1965 0.48 -0.0 2,079,742 - Psest_1965 0.84 -0.5 2,079,927 + +0.3 2,079,927 + -0.2 2,079,928 - +0.4 2,079,928 - +0.1 2,079,928 - -2.6 2,079,928 - +0.6 2,079,928 - -1.3 2,079,930 + +0.8 2,079,931 - -2.2 2,079,931 - -1.8 2,079,931 - -1.6 2,079,988 - +3.3 2,080,069 + Psest_1966 0.13 +0.0 2,080,070 - Psest_1966 0.13 +3.5 2,080,071 + Psest_1966 0.13 +0.5 2,080,071 + Psest_1966 0.13 +1.0 2,080,071 + Psest_1966 0.13 -1.5 2,080,072 - Psest_1966 0.13 +0.1 2,080,072 - Psest_1966 0.13 +0.3 2,080,072 - Psest_1966 0.13 -2.4 2,080,072 - Psest_1966 0.13 +2.7 2,080,072 - Psest_1966 0.13 -1.8 2,080,072 - Psest_1966 0.13 -1.0 2,080,105 - Psest_1966 0.16 +0.1 2,080,107 + Psest_1966 0.16 -0.9 2,080,107 + Psest_1966 0.16 -0.2 2,080,107 + Psest_1966 0.16 -1.2 2,080,107 + Psest_1966 0.16 +0.1 2,080,107 + Psest_1966 0.16 -1.2 2,080,107 + Psest_1966 0.16 -1.1 2,080,107 + Psest_1966 0.16 +0.7 2,080,107 + Psest_1966 0.16 -0.8 2,080,107 + Psest_1966 0.16 +0.1 2,080,108 - Psest_1966 0.16 -1.0 2,080,108 - Psest_1966 0.16 -1.1 2,080,108 - Psest_1966 0.16 -1.5 2,080,108 - Psest_1966 0.16 -0.5 2,080,108 - Psest_1966 0.16 +6.2 2,080,108 - Psest_1966 0.16 +0.2 2,080,108 - Psest_1966 0.16 -0.6 2,080,108 - Psest_1966 0.16 +0.3 2,080,108 - Psest_1966 0.16 -0.8 2,080,108 - Psest_1966 0.16 -2.9 2,080,108 - Psest_1966 0.16 -0.4 2,080,112 - Psest_1966 0.17 +0.5 2,080,179 + Psest_1966 0.22 -0.3 2,080,179 + Psest_1966 0.22 +0.1 2,080,180 - Psest_1966 0.22 +0.9 2,080,216 + Psest_1966 0.25 -1.0 2,080,269 + Psest_1966 0.29 +2.2 2,080,270 - Psest_1966 0.29 -0.8 2,080,287 + Psest_1966 0.30 +2.6 2,080,287 + Psest_1966 0.30 +0.8 2,080,287 + Psest_1966 0.30 -1.5 2,080,288 - Psest_1966 0.30 -1.1 2,080,320 + Psest_1966 0.33 -0.2 2,080,320 + Psest_1966 0.33 +0.5 2,080,320 + Psest_1966 0.33 -0.7 2,080,332 + Psest_1966 0.34 +0.4 2,080,468 - Psest_1966 0.44 +1.7 2,080,468 - Psest_1966 0.44 -0.5 2,080,519 + Psest_1966 0.48 -0.2 2,080,520 - Psest_1966 0.48 +0.1 2,080,697 + Psest_1966 0.62 +1.1 2,080,705 + Psest_1966 0.63 +0.3 2,080,706 - Psest_1966 0.63 +0.2 2,080,802 + Psest_1966 0.70 -0.8 2,080,802 + Psest_1966 0.70 +5.4 2,080,803 - Psest_1966 0.70 +0.1 2,080,831 + Psest_1966 0.73 -1.5 2,080,836 + Psest_1966 0.73 +0.7 2,080,837 - Psest_1966 0.73 +1.8
Or see this region's nucleotide sequence