Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1825 and Psest_1826 are separated by 213 nucleotides Psest_1826 and Psest_1827 are separated by 283 nucleotides
Psest_1825: Psest_1825 - Predicted nucleoside-diphosphate sugar epimerases, at 1,923,758 to 1,925,773
_1825
Psest_1826: Psest_1826 - hypothetical protein, at 1,925,987 to 1,926,394
_1826
Psest_1827: Psest_1827 - Phosphopantetheinyl transferase component of siderophore synthetase, at 1,926,678 to 1,927,415
_1827
Position (kb)
1925
1926
1927 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1925.255 kb on + strand, within Psest_1825 at 1925.255 kb on + strand, within Psest_1825 at 1925.256 kb on - strand, within Psest_1825 at 1925.312 kb on + strand, within Psest_1825 at 1925.312 kb on + strand, within Psest_1825 at 1925.488 kb on + strand, within Psest_1825 at 1925.501 kb on + strand, within Psest_1825 at 1925.531 kb on + strand, within Psest_1825 at 1925.532 kb on - strand, within Psest_1825 at 1925.532 kb on - strand, within Psest_1825 at 1925.541 kb on + strand, within Psest_1825 at 1925.542 kb on - strand, within Psest_1825 at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.619 kb on + strand at 1925.620 kb on - strand at 1925.620 kb on - strand at 1925.745 kb on - strand at 1925.772 kb on - strand at 1925.774 kb on + strand at 1925.774 kb on + strand at 1925.774 kb on + strand at 1925.774 kb on + strand at 1925.774 kb on + strand at 1925.775 kb on - strand at 1925.775 kb on - strand at 1925.775 kb on - strand at 1925.775 kb on - strand at 1925.775 kb on - strand at 1925.778 kb on + strand at 1925.778 kb on + strand at 1925.778 kb on + strand at 1925.778 kb on + strand at 1925.779 kb on - strand at 1925.784 kb on + strand at 1925.785 kb on - strand at 1925.785 kb on - strand at 1925.884 kb on + strand at 1925.953 kb on + strand at 1925.953 kb on + strand at 1925.953 kb on + strand at 1925.954 kb on - strand at 1926.155 kb on + strand, within Psest_1826 at 1926.155 kb on + strand, within Psest_1826 at 1926.156 kb on - strand, within Psest_1826 at 1926.156 kb on - strand, within Psest_1826 at 1926.156 kb on - strand, within Psest_1826 at 1926.156 kb on - strand, within Psest_1826 at 1926.430 kb on - strand at 1926.430 kb on - strand at 1926.432 kb on - strand at 1926.432 kb on - strand at 1926.432 kb on - strand at 1926.552 kb on + strand at 1926.552 kb on + strand at 1926.553 kb on - strand at 1926.614 kb on + strand at 1926.615 kb on - strand at 1926.615 kb on - strand at 1926.615 kb on - strand at 1926.646 kb on + strand at 1926.646 kb on + strand at 1926.679 kb on + strand at 1926.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 1,925,255 + Psest_1825 0.74 -1.3 1,925,255 + Psest_1825 0.74 -1.1 1,925,256 - Psest_1825 0.74 -0.5 1,925,312 + Psest_1825 0.77 -3.0 1,925,312 + Psest_1825 0.77 +0.6 1,925,488 + Psest_1825 0.86 -0.2 1,925,501 + Psest_1825 0.86 +2.8 1,925,531 + Psest_1825 0.88 -0.6 1,925,532 - Psest_1825 0.88 -0.1 1,925,532 - Psest_1825 0.88 -0.6 1,925,541 + Psest_1825 0.88 +1.7 1,925,542 - Psest_1825 0.88 +0.2 1,925,619 + -1.5 1,925,619 + +0.0 1,925,619 + -1.8 1,925,619 + +0.3 1,925,619 + +0.8 1,925,619 + +1.2 1,925,619 + -0.5 1,925,620 - +0.7 1,925,620 - +1.0 1,925,745 - +0.4 1,925,772 - -1.4 1,925,774 + +0.7 1,925,774 + -0.6 1,925,774 + +2.8 1,925,774 + +1.2 1,925,774 + -0.6 1,925,775 - +0.1 1,925,775 - +0.5 1,925,775 - -0.4 1,925,775 - +1.7 1,925,775 - -0.4 1,925,778 + -1.8 1,925,778 + +0.3 1,925,778 + -0.1 1,925,778 + -0.1 1,925,779 - +0.4 1,925,784 + -0.6 1,925,785 - -2.3 1,925,785 - -0.9 1,925,884 + +0.6 1,925,953 + -1.4 1,925,953 + -1.6 1,925,953 + +1.1 1,925,954 - +0.0 1,926,155 + Psest_1826 0.41 -0.3 1,926,155 + Psest_1826 0.41 +0.3 1,926,156 - Psest_1826 0.41 -2.2 1,926,156 - Psest_1826 0.41 -1.6 1,926,156 - Psest_1826 0.41 -0.7 1,926,156 - Psest_1826 0.41 +0.3 1,926,430 - +0.1 1,926,430 - +0.2 1,926,432 - -0.1 1,926,432 - +0.5 1,926,432 - -1.5 1,926,552 + +1.1 1,926,552 + -0.7 1,926,553 - +1.3 1,926,614 + +0.4 1,926,615 - -0.8 1,926,615 - -1.5 1,926,615 - -0.8 1,926,646 + +1.9 1,926,646 + -2.5 1,926,679 + +0.1 1,926,680 - +0.3
Or see this region's nucleotide sequence