Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1620 and Psest_1621 are separated by 85 nucleotides Psest_1621 and Psest_1622 are separated by 9 nucleotides Psest_1622 and Psest_1623 are separated by 94 nucleotides
Psest_1620: Psest_1620 - Phosphate-selective porin, at 1,723,709 to 1,724,998
_1620
Psest_1621: Psest_1621 - Uncharacterized protein conserved in bacteria, at 1,725,084 to 1,726,214
_1621
Psest_1622: Psest_1622 - hypothetical protein, at 1,726,224 to 1,726,646
_1622
Psest_1623: Psest_1623 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 1,726,741 to 1,727,295
_1623
Position (kb)
1725
1726
1727 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1724.174 kb on + strand, within Psest_1620 at 1724.174 kb on + strand, within Psest_1620 at 1724.174 kb on + strand, within Psest_1620 at 1724.175 kb on - strand, within Psest_1620 at 1724.175 kb on - strand, within Psest_1620 at 1724.175 kb on - strand, within Psest_1620 at 1724.555 kb on + strand, within Psest_1620 at 1724.555 kb on + strand, within Psest_1620 at 1724.555 kb on + strand, within Psest_1620 at 1724.555 kb on + strand, within Psest_1620 at 1724.555 kb on + strand, within Psest_1620 at 1724.589 kb on - strand, within Psest_1620 at 1724.589 kb on - strand, within Psest_1620 at 1724.646 kb on - strand, within Psest_1620 at 1724.646 kb on - strand, within Psest_1620 at 1724.663 kb on + strand, within Psest_1620 at 1724.663 kb on + strand, within Psest_1620 at 1724.690 kb on + strand, within Psest_1620 at 1724.690 kb on + strand, within Psest_1620 at 1724.690 kb on + strand, within Psest_1620 at 1724.690 kb on + strand, within Psest_1620 at 1724.691 kb on - strand, within Psest_1620 at 1724.691 kb on - strand, within Psest_1620 at 1724.691 kb on - strand, within Psest_1620 at 1724.814 kb on - strand, within Psest_1620 at 1724.909 kb on + strand at 1724.909 kb on + strand at 1724.910 kb on - strand at 1724.910 kb on - strand at 1724.987 kb on + strand at 1724.996 kb on + strand at 1724.997 kb on - strand at 1724.997 kb on - strand at 1724.997 kb on - strand at 1725.306 kb on + strand, within Psest_1621 at 1725.307 kb on - strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.315 kb on + strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.316 kb on - strand, within Psest_1621 at 1725.369 kb on + strand, within Psest_1621 at 1725.656 kb on - strand, within Psest_1621 at 1725.747 kb on + strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.769 kb on - strand, within Psest_1621 at 1725.918 kb on + strand, within Psest_1621 at 1726.092 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.096 kb on + strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.097 kb on - strand, within Psest_1621 at 1726.212 kb on + strand at 1726.212 kb on + strand at 1726.213 kb on - strand at 1726.316 kb on - strand, within Psest_1622 at 1726.389 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.406 kb on - strand, within Psest_1622 at 1726.584 kb on + strand, within Psest_1622 at 1726.584 kb on + strand, within Psest_1622 at 1726.585 kb on - strand, within Psest_1622 at 1726.694 kb on - strand at 1726.705 kb on + strand at 1726.784 kb on - strand at 1726.852 kb on + strand, within Psest_1623 at 1726.853 kb on - strand, within Psest_1623 at 1727.117 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.131 kb on + strand, within Psest_1623 at 1727.132 kb on - strand, within Psest_1623 at 1727.136 kb on - strand, within Psest_1623
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 1,724,174 + Psest_1620 0.36 +0.8 1,724,174 + Psest_1620 0.36 -1.0 1,724,174 + Psest_1620 0.36 -1.4 1,724,175 - Psest_1620 0.36 +0.8 1,724,175 - Psest_1620 0.36 +0.9 1,724,175 - Psest_1620 0.36 -2.0 1,724,555 + Psest_1620 0.66 -0.6 1,724,555 + Psest_1620 0.66 -0.4 1,724,555 + Psest_1620 0.66 +0.7 1,724,555 + Psest_1620 0.66 -1.0 1,724,555 + Psest_1620 0.66 -0.9 1,724,589 - Psest_1620 0.68 +1.9 1,724,589 - Psest_1620 0.68 +2.8 1,724,646 - Psest_1620 0.73 +2.0 1,724,646 - Psest_1620 0.73 +2.1 1,724,663 + Psest_1620 0.74 +2.4 1,724,663 + Psest_1620 0.74 +0.9 1,724,690 + Psest_1620 0.76 +1.0 1,724,690 + Psest_1620 0.76 -0.2 1,724,690 + Psest_1620 0.76 -0.6 1,724,690 + Psest_1620 0.76 -0.3 1,724,691 - Psest_1620 0.76 -0.7 1,724,691 - Psest_1620 0.76 -1.3 1,724,691 - Psest_1620 0.76 -0.7 1,724,814 - Psest_1620 0.86 +2.8 1,724,909 + -2.0 1,724,909 + +1.0 1,724,910 - +0.5 1,724,910 - -0.9 1,724,987 + +0.9 1,724,996 + -0.3 1,724,997 - +0.4 1,724,997 - -1.6 1,724,997 - -1.2 1,725,306 + Psest_1621 0.20 -0.6 1,725,307 - Psest_1621 0.20 -0.1 1,725,315 + Psest_1621 0.20 -0.2 1,725,315 + Psest_1621 0.20 +0.2 1,725,315 + Psest_1621 0.20 +1.3 1,725,315 + Psest_1621 0.20 +2.2 1,725,316 - Psest_1621 0.21 -0.1 1,725,316 - Psest_1621 0.21 +0.3 1,725,316 - Psest_1621 0.21 +0.6 1,725,316 - Psest_1621 0.21 +1.9 1,725,369 + Psest_1621 0.25 -2.3 1,725,656 - Psest_1621 0.51 -0.6 1,725,747 + Psest_1621 0.59 +0.2 1,725,769 - Psest_1621 0.61 -1.0 1,725,769 - Psest_1621 0.61 +0.4 1,725,769 - Psest_1621 0.61 -0.7 1,725,769 - Psest_1621 0.61 +0.3 1,725,918 + Psest_1621 0.74 +1.1 1,726,092 + Psest_1621 0.89 +2.1 1,726,096 + Psest_1621 0.89 +1.7 1,726,096 + Psest_1621 0.89 -0.8 1,726,096 + Psest_1621 0.89 -0.9 1,726,096 + Psest_1621 0.89 -0.2 1,726,096 + Psest_1621 0.89 +0.8 1,726,096 + Psest_1621 0.89 +4.4 1,726,096 + Psest_1621 0.89 +0.5 1,726,096 + Psest_1621 0.89 -1.2 1,726,097 - Psest_1621 0.90 +4.2 1,726,097 - Psest_1621 0.90 +3.0 1,726,097 - Psest_1621 0.90 -0.8 1,726,212 + +0.4 1,726,212 + +1.2 1,726,213 - -1.8 1,726,316 - Psest_1622 0.22 -0.3 1,726,389 - Psest_1622 0.39 -1.0 1,726,406 - Psest_1622 0.43 -1.4 1,726,406 - Psest_1622 0.43 +0.1 1,726,406 - Psest_1622 0.43 +1.3 1,726,584 + Psest_1622 0.85 +0.8 1,726,584 + Psest_1622 0.85 +0.4 1,726,585 - Psest_1622 0.85 -0.1 1,726,694 - -0.0 1,726,705 + -0.1 1,726,784 - -1.6 1,726,852 + Psest_1623 0.20 +1.0 1,726,853 - Psest_1623 0.20 -1.4 1,727,117 + Psest_1623 0.68 -1.8 1,727,131 + Psest_1623 0.70 +0.5 1,727,131 + Psest_1623 0.70 -1.1 1,727,131 + Psest_1623 0.70 -1.1 1,727,132 - Psest_1623 0.70 -0.3 1,727,136 - Psest_1623 0.71 +1.2
Or see this region's nucleotide sequence