Experiment: RCH2 grown on a colony of Pseudomonas migulae strain N1B4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1390 and Psest_1391 are separated by 65 nucleotides Psest_1391 and Psest_1392 are separated by 12 nucleotides
Psest_1390: Psest_1390 - Uncharacterized ABC-type transport system, permease component, at 1,498,524 to 1,499,450
_1390
Psest_1391: Psest_1391 - ABC-type uncharacterized transport system, permease component, at 1,499,516 to 1,500,622
_1391
Psest_1392: Psest_1392 - ABC-type uncharacterized transport systems, ATPase components, at 1,500,635 to 1,502,191
_1392
Position (kb)
1499
1500
1501 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1498.568 kb on - strand at 1498.627 kb on + strand, within Psest_1390 at 1498.714 kb on + strand, within Psest_1390 at 1498.835 kb on - strand, within Psest_1390 at 1498.835 kb on - strand, within Psest_1390 at 1499.014 kb on + strand, within Psest_1390 at 1499.014 kb on + strand, within Psest_1390 at 1499.014 kb on + strand, within Psest_1390 at 1499.014 kb on + strand, within Psest_1390 at 1499.015 kb on - strand, within Psest_1390 at 1499.015 kb on - strand, within Psest_1390 at 1499.346 kb on + strand, within Psest_1390 at 1499.426 kb on + strand at 1499.427 kb on - strand at 1499.427 kb on - strand at 1499.427 kb on - strand at 1500.015 kb on + strand, within Psest_1391 at 1500.016 kb on - strand, within Psest_1391 at 1500.159 kb on + strand, within Psest_1391 at 1500.159 kb on + strand, within Psest_1391 at 1500.159 kb on + strand, within Psest_1391 at 1500.159 kb on + strand, within Psest_1391 at 1500.159 kb on + strand, within Psest_1391 at 1500.160 kb on - strand, within Psest_1391 at 1500.160 kb on - strand, within Psest_1391 at 1500.160 kb on - strand, within Psest_1391 at 1500.381 kb on + strand, within Psest_1391 at 1500.381 kb on + strand, within Psest_1391 at 1500.382 kb on - strand, within Psest_1391 at 1500.451 kb on - strand, within Psest_1391
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Pseudomonas migulae strain N1B4 remove 1,498,568 - -2.7 1,498,627 + Psest_1390 0.11 -2.5 1,498,714 + Psest_1390 0.20 +5.2 1,498,835 - Psest_1390 0.34 +0.6 1,498,835 - Psest_1390 0.34 +2.1 1,499,014 + Psest_1390 0.53 -0.0 1,499,014 + Psest_1390 0.53 +0.7 1,499,014 + Psest_1390 0.53 +1.2 1,499,014 + Psest_1390 0.53 +2.6 1,499,015 - Psest_1390 0.53 -0.3 1,499,015 - Psest_1390 0.53 -2.2 1,499,346 + Psest_1390 0.89 +0.3 1,499,426 + +1.5 1,499,427 - +3.2 1,499,427 - -1.6 1,499,427 - -2.7 1,500,015 + Psest_1391 0.45 +0.3 1,500,016 - Psest_1391 0.45 +0.5 1,500,159 + Psest_1391 0.58 -1.6 1,500,159 + Psest_1391 0.58 -0.1 1,500,159 + Psest_1391 0.58 +0.4 1,500,159 + Psest_1391 0.58 +1.5 1,500,159 + Psest_1391 0.58 +0.1 1,500,160 - Psest_1391 0.58 -0.6 1,500,160 - Psest_1391 0.58 +0.3 1,500,160 - Psest_1391 0.58 -1.1 1,500,381 + Psest_1391 0.78 -1.6 1,500,381 + Psest_1391 0.78 +1.6 1,500,382 - Psest_1391 0.78 +1.7 1,500,451 - Psest_1391 0.84 +1.5
Or see this region's nucleotide sequence