Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0855 and Psest_0856 are separated by 12 nucleotides Psest_0856 and Psest_0857 are separated by 275 nucleotides Psest_0857 and Psest_0858 are separated by 251 nucleotides
Psest_0855: Psest_0855 - NAD-dependent aldehyde dehydrogenases, at 925,527 to 927,155
_0855
Psest_0856: Psest_0856 - Predicted oxidoreductase, at 927,168 to 928,031
_0856
Psest_0857: Psest_0857 - Membrane transporters of cations and cationic drugs, at 928,307 to 928,627
_0857
Psest_0858: Psest_0858 - Maltose operon periplasmic protein precursor (MalM)., at 928,879 to 929,802
_0858
Position (kb) 927
928
929 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 926.766 kb on + strand, within Psest_0855 at 926.835 kb on + strand, within Psest_0855 at 926.836 kb on - strand, within Psest_0855 at 926.836 kb on - strand, within Psest_0855 at 926.836 kb on - strand, within Psest_0855 at 926.836 kb on - strand, within Psest_0855 at 926.836 kb on - strand, within Psest_0855 at 926.977 kb on - strand, within Psest_0855 at 927.022 kb on + strand at 927.022 kb on + strand at 927.022 kb on + strand at 927.023 kb on - strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.144 kb on + strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.145 kb on - strand at 927.315 kb on + strand, within Psest_0856 at 927.315 kb on + strand, within Psest_0856 at 927.315 kb on + strand, within Psest_0856 at 927.315 kb on + strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.316 kb on - strand, within Psest_0856 at 927.453 kb on + strand, within Psest_0856 at 927.454 kb on - strand, within Psest_0856 at 927.725 kb on - strand, within Psest_0856 at 927.725 kb on - strand, within Psest_0856 at 927.993 kb on + strand at 927.993 kb on + strand at 927.993 kb on + strand at 927.993 kb on + strand at 928.101 kb on + strand at 928.102 kb on - strand at 928.102 kb on - strand at 928.102 kb on - strand at 928.104 kb on + strand at 928.105 kb on - strand at 928.105 kb on - strand at 928.105 kb on - strand at 928.168 kb on + strand at 928.227 kb on - strand at 928.488 kb on - strand, within Psest_0857 at 928.488 kb on - strand, within Psest_0857 at 928.668 kb on + strand at 928.668 kb on + strand at 928.669 kb on - strand at 928.669 kb on - strand at 928.718 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.750 kb on - strand at 928.750 kb on - strand at 928.804 kb on + strand at 928.826 kb on + strand at 929.012 kb on + strand, within Psest_0858 at 929.012 kb on + strand, within Psest_0858 at 929.012 kb on + strand, within Psest_0858 at 929.013 kb on - strand, within Psest_0858
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 926,766 + Psest_0855 0.76 +2.5 926,835 + Psest_0855 0.80 -0.8 926,836 - Psest_0855 0.80 +0.4 926,836 - Psest_0855 0.80 +0.7 926,836 - Psest_0855 0.80 +3.5 926,836 - Psest_0855 0.80 -1.0 926,836 - Psest_0855 0.80 +2.1 926,977 - Psest_0855 0.89 +1.3 927,022 + +0.4 927,022 + +0.0 927,022 + -0.1 927,023 - -1.6 927,144 + +1.5 927,144 + -0.4 927,144 + +1.4 927,144 + +1.0 927,144 + -0.6 927,144 + +0.7 927,144 + +0.9 927,144 + +0.3 927,145 - -0.6 927,145 - -1.9 927,145 - -0.2 927,145 - +0.1 927,145 - -1.2 927,145 - +0.4 927,145 - +0.6 927,315 + Psest_0856 0.17 -2.3 927,315 + Psest_0856 0.17 -0.3 927,315 + Psest_0856 0.17 -1.3 927,315 + Psest_0856 0.17 -0.6 927,316 - Psest_0856 0.17 -1.2 927,316 - Psest_0856 0.17 -1.2 927,316 - Psest_0856 0.17 -0.5 927,316 - Psest_0856 0.17 +0.4 927,316 - Psest_0856 0.17 -0.1 927,316 - Psest_0856 0.17 -1.4 927,453 + Psest_0856 0.33 -1.9 927,454 - Psest_0856 0.33 -1.5 927,725 - Psest_0856 0.64 -0.8 927,725 - Psest_0856 0.64 +0.8 927,993 + -0.5 927,993 + -0.2 927,993 + +1.5 927,993 + +0.3 928,101 + +0.8 928,102 - +1.8 928,102 - +1.1 928,102 - +2.1 928,104 + +0.3 928,105 - -1.0 928,105 - +0.4 928,105 - -0.3 928,168 + +0.3 928,227 - -0.1 928,488 - Psest_0857 0.56 +0.9 928,488 - Psest_0857 0.56 -0.2 928,668 + +1.4 928,668 + +3.7 928,669 - +0.4 928,669 - -0.5 928,718 + +0.5 928,747 + -0.1 928,747 + +0.5 928,747 + -1.0 928,747 + +1.8 928,747 + +0.4 928,747 + -0.1 928,748 - -1.5 928,748 - -1.0 928,748 - -1.1 928,748 - +1.2 928,748 - +0.1 928,748 - +1.3 928,748 - +0.2 928,748 - +1.0 928,748 - +0.2 928,748 - +0.5 928,748 - +0.9 928,748 - +0.3 928,750 - -0.8 928,750 - -0.9 928,804 + -1.7 928,826 + -0.6 929,012 + Psest_0858 0.14 +0.0 929,012 + Psest_0858 0.14 -1.3 929,012 + Psest_0858 0.14 -0.9 929,013 - Psest_0858 0.15 -2.4
Or see this region's nucleotide sequence