Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0776 and Psest_0777 are separated by 4 nucleotides Psest_0777 and Psest_0778 are separated by 84 nucleotides Psest_0778 and Psest_0779 are separated by 195 nucleotides
Psest_0776: Psest_0776 - FOG: Ankyrin repeat, at 845,785 to 846,339
_0776
Psest_0777: Psest_0777 - cyanate hydratase, at 846,344 to 846,793
_0777
Psest_0778: Psest_0778 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 846,878 to 847,639
_0778
Psest_0779: Psest_0779 - tyrosine aminotransferase (EC 2.6.1.57) <small>(from data)</small>, at 847,835 to 849,031
_0779
Position (kb) 846
847
848 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 845.906 kb on - strand, within Psest_0776 at 845.998 kb on + strand, within Psest_0776 at 845.999 kb on - strand, within Psest_0776 at 846.022 kb on + strand, within Psest_0776 at 846.023 kb on - strand, within Psest_0776 at 846.023 kb on - strand, within Psest_0776 at 846.023 kb on - strand, within Psest_0776 at 846.211 kb on + strand, within Psest_0776 at 846.211 kb on + strand, within Psest_0776 at 846.212 kb on + strand, within Psest_0776 at 846.212 kb on - strand, within Psest_0776 at 846.212 kb on - strand, within Psest_0776 at 846.212 kb on - strand, within Psest_0776 at 846.212 kb on - strand, within Psest_0776 at 846.212 kb on - strand, within Psest_0776 at 846.348 kb on + strand at 846.348 kb on + strand at 846.349 kb on - strand at 846.519 kb on + strand, within Psest_0777 at 846.528 kb on + strand, within Psest_0777 at 846.573 kb on + strand, within Psest_0777 at 846.574 kb on - strand, within Psest_0777 at 846.574 kb on - strand, within Psest_0777 at 846.743 kb on + strand, within Psest_0777 at 846.743 kb on + strand, within Psest_0777 at 846.743 kb on + strand, within Psest_0777 at 846.743 kb on + strand, within Psest_0777 at 846.743 kb on + strand, within Psest_0777 at 846.744 kb on - strand, within Psest_0777 at 846.744 kb on - strand, within Psest_0777 at 846.885 kb on + strand at 846.885 kb on + strand at 846.973 kb on - strand, within Psest_0778 at 846.973 kb on - strand, within Psest_0778 at 847.020 kb on - strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.231 kb on - strand, within Psest_0778 at 847.375 kb on + strand, within Psest_0778 at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.836 kb on + strand at 847.836 kb on + strand at 847.837 kb on - strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.930 kb on - strand at 847.959 kb on - strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.984 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 845,906 - Psest_0776 0.22 +1.3 845,998 + Psest_0776 0.38 +0.6 845,999 - Psest_0776 0.39 -1.4 846,022 + Psest_0776 0.43 -0.0 846,023 - Psest_0776 0.43 +0.2 846,023 - Psest_0776 0.43 +1.0 846,023 - Psest_0776 0.43 -0.9 846,211 + Psest_0776 0.77 +3.6 846,211 + Psest_0776 0.77 +0.7 846,212 + Psest_0776 0.77 +0.0 846,212 - Psest_0776 0.77 +5.1 846,212 - Psest_0776 0.77 -0.5 846,212 - Psest_0776 0.77 +0.3 846,212 - Psest_0776 0.77 -0.0 846,212 - Psest_0776 0.77 +0.7 846,348 + +1.3 846,348 + -0.8 846,349 - +0.3 846,519 + Psest_0777 0.39 +1.3 846,528 + Psest_0777 0.41 +1.9 846,573 + Psest_0777 0.51 -1.3 846,574 - Psest_0777 0.51 +0.3 846,574 - Psest_0777 0.51 -0.4 846,743 + Psest_0777 0.89 -1.3 846,743 + Psest_0777 0.89 -0.6 846,743 + Psest_0777 0.89 -0.3 846,743 + Psest_0777 0.89 +0.0 846,743 + Psest_0777 0.89 +0.1 846,744 - Psest_0777 0.89 +3.2 846,744 - Psest_0777 0.89 -0.8 846,885 + +1.3 846,885 + +0.1 846,973 - Psest_0778 0.12 -0.2 846,973 - Psest_0778 0.12 -2.8 847,020 - Psest_0778 0.19 -0.1 847,230 + Psest_0778 0.46 -0.2 847,230 + Psest_0778 0.46 -1.8 847,230 + Psest_0778 0.46 -0.1 847,231 - Psest_0778 0.46 -1.9 847,375 + Psest_0778 0.65 +0.3 847,671 + +1.0 847,671 + +0.8 847,671 + -0.5 847,671 + -1.1 847,671 + -0.2 847,672 - +1.7 847,672 - -2.1 847,672 - -0.5 847,836 + -1.3 847,836 + -0.5 847,837 - -2.0 847,929 + -0.4 847,929 + -0.4 847,929 + -0.9 847,929 + -0.9 847,929 + -1.4 847,930 - -0.9 847,959 - Psest_0779 0.10 +1.6 847,983 + Psest_0779 0.12 +0.2 847,983 + Psest_0779 0.12 -1.2 847,983 + Psest_0779 0.12 -0.5 847,984 - Psest_0779 0.12 -0.9 848,584 - Psest_0779 0.63 -0.1 848,584 - Psest_0779 0.63 +1.3
Or see this region's nucleotide sequence