Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0642 and Psest_0643 overlap by 4 nucleotides Psest_0643 and Psest_0644 are separated by 138 nucleotides Psest_0644 and Psest_0645 overlap by 17 nucleotides
Psest_0642: Psest_0642 - Geranylgeranyl pyrophosphate synthase, at 697,434 to 698,324
_0642
Psest_0643: Psest_0643 - exodeoxyribonuclease VII, small subunit, at 698,321 to 698,563
_0643
Psest_0644: Psest_0644 - siderophore ferric iron reductase, AHA_1954 family, at 698,702 to 699,463
_0644
Psest_0645: Psest_0645 - Siderophore synthetase component, at 699,447 to 701,255
_0645
Position (kb) 698
699
700 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 698.607 kb on + strand at 698.608 kb on - strand at 698.655 kb on + strand at 698.656 kb on - strand at 698.656 kb on - strand at 698.668 kb on - strand at 698.668 kb on - strand at 699.200 kb on + strand, within Psest_0644 at 699.200 kb on + strand, within Psest_0644 at 699.220 kb on - strand, within Psest_0644 at 699.220 kb on - strand, within Psest_0644 at 699.220 kb on - strand, within Psest_0644 at 699.220 kb on - strand, within Psest_0644 at 699.264 kb on + strand, within Psest_0644 at 699.264 kb on + strand, within Psest_0644 at 699.399 kb on - strand at 699.463 kb on + strand at 699.463 kb on + strand at 699.464 kb on - strand at 699.643 kb on + strand, within Psest_0645 at 699.946 kb on + strand, within Psest_0645 at 699.947 kb on - strand, within Psest_0645 at 699.947 kb on - strand, within Psest_0645 at 699.947 kb on - strand, within Psest_0645 at 699.947 kb on - strand, within Psest_0645 at 699.973 kb on + strand, within Psest_0645 at 699.974 kb on - strand, within Psest_0645 at 699.974 kb on - strand, within Psest_0645 at 700.186 kb on + strand, within Psest_0645 at 700.189 kb on + strand, within Psest_0645 at 700.190 kb on - strand, within Psest_0645 at 700.282 kb on + strand, within Psest_0645 at 700.282 kb on + strand, within Psest_0645 at 700.282 kb on + strand, within Psest_0645 at 700.282 kb on + strand, within Psest_0645 at 700.283 kb on - strand, within Psest_0645 at 700.283 kb on - strand, within Psest_0645 at 700.283 kb on - strand, within Psest_0645 at 700.283 kb on - strand, within Psest_0645 at 700.283 kb on - strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.312 kb on + strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.313 kb on - strand, within Psest_0645 at 700.453 kb on + strand, within Psest_0645 at 700.453 kb on + strand, within Psest_0645 at 700.454 kb on - strand, within Psest_0645 at 700.454 kb on - strand, within Psest_0645 at 700.454 kb on - strand, within Psest_0645
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 698,607 + +0.7 698,608 - +0.2 698,655 + +0.5 698,656 - +0.1 698,656 - -0.8 698,668 - +1.0 698,668 - -0.8 699,200 + Psest_0644 0.65 +0.5 699,200 + Psest_0644 0.65 +0.7 699,220 - Psest_0644 0.68 -1.6 699,220 - Psest_0644 0.68 -2.7 699,220 - Psest_0644 0.68 -2.1 699,220 - Psest_0644 0.68 -0.5 699,264 + Psest_0644 0.74 -0.0 699,264 + Psest_0644 0.74 -0.0 699,399 - +1.3 699,463 + -0.3 699,463 + -1.0 699,464 - -0.0 699,643 + Psest_0645 0.11 -1.5 699,946 + Psest_0645 0.28 -1.4 699,947 - Psest_0645 0.28 +1.1 699,947 - Psest_0645 0.28 -0.6 699,947 - Psest_0645 0.28 -0.9 699,947 - Psest_0645 0.28 -1.2 699,973 + Psest_0645 0.29 +2.3 699,974 - Psest_0645 0.29 +2.3 699,974 - Psest_0645 0.29 +0.4 700,186 + Psest_0645 0.41 +1.1 700,189 + Psest_0645 0.41 +0.2 700,190 - Psest_0645 0.41 +0.1 700,282 + Psest_0645 0.46 +0.5 700,282 + Psest_0645 0.46 +0.9 700,282 + Psest_0645 0.46 +0.4 700,282 + Psest_0645 0.46 +2.7 700,283 - Psest_0645 0.46 -1.4 700,283 - Psest_0645 0.46 -0.5 700,283 - Psest_0645 0.46 +2.1 700,283 - Psest_0645 0.46 -1.1 700,283 - Psest_0645 0.46 +0.1 700,312 + Psest_0645 0.48 -1.4 700,312 + Psest_0645 0.48 -1.0 700,312 + Psest_0645 0.48 -2.9 700,312 + Psest_0645 0.48 -2.1 700,312 + Psest_0645 0.48 +0.5 700,312 + Psest_0645 0.48 +1.1 700,312 + Psest_0645 0.48 +1.1 700,312 + Psest_0645 0.48 +4.0 700,312 + Psest_0645 0.48 -0.5 700,312 + Psest_0645 0.48 -0.6 700,312 + Psest_0645 0.48 -1.8 700,312 + Psest_0645 0.48 +0.3 700,312 + Psest_0645 0.48 -2.1 700,312 + Psest_0645 0.48 -0.5 700,312 + Psest_0645 0.48 +1.1 700,312 + Psest_0645 0.48 +1.0 700,312 + Psest_0645 0.48 -1.7 700,312 + Psest_0645 0.48 +0.9 700,312 + Psest_0645 0.48 -0.3 700,312 + Psest_0645 0.48 -0.1 700,313 - Psest_0645 0.48 +0.3 700,313 - Psest_0645 0.48 +0.3 700,313 - Psest_0645 0.48 -1.0 700,313 - Psest_0645 0.48 -0.1 700,313 - Psest_0645 0.48 +0.4 700,313 - Psest_0645 0.48 +1.1 700,313 - Psest_0645 0.48 -0.2 700,313 - Psest_0645 0.48 -0.2 700,313 - Psest_0645 0.48 +0.1 700,313 - Psest_0645 0.48 -0.0 700,313 - Psest_0645 0.48 -0.7 700,313 - Psest_0645 0.48 +0.4 700,313 - Psest_0645 0.48 -0.2 700,453 + Psest_0645 0.56 -0.5 700,453 + Psest_0645 0.56 +0.5 700,454 - Psest_0645 0.56 -0.9 700,454 - Psest_0645 0.56 -0.6 700,454 - Psest_0645 0.56 +0.1
Or see this region's nucleotide sequence