Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0412

Experiment: RCH2 grown on a colony of N2A2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0411 and Psest_0412 are separated by 107 nucleotidesPsest_0412 and Psest_0413 overlap by 8 nucleotidesPsest_0413 and Psest_0414 overlap by 8 nucleotides Psest_0411: Psest_0411 - biotin synthetase, at 446,599 to 447,654 _0411 Psest_0412: Psest_0412 - 8-amino-7-oxononanoate synthase, at 447,762 to 448,940 _0412 Psest_0413: Psest_0413 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 448,933 to 449,658 _0413 Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445 _0414 Position (kb) 447 448 449Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 446.791 kb on + strand, within Psest_0411at 446.791 kb on + strand, within Psest_0411at 446.791 kb on + strand, within Psest_0411at 446.791 kb on + strand, within Psest_0411at 446.791 kb on + strand, within Psest_0411at 446.791 kb on + strand, within Psest_0411at 446.792 kb on - strand, within Psest_0411at 446.813 kb on - strand, within Psest_0411at 446.813 kb on - strand, within Psest_0411at 446.813 kb on - strand, within Psest_0411at 446.950 kb on + strand, within Psest_0411at 446.951 kb on - strand, within Psest_0411at 446.951 kb on - strand, within Psest_0411at 447.062 kb on - strand, within Psest_0411at 447.064 kb on + strand, within Psest_0411at 447.064 kb on + strand, within Psest_0411at 447.064 kb on + strand, within Psest_0411at 447.064 kb on + strand, within Psest_0411at 447.064 kb on + strand, within Psest_0411at 447.065 kb on - strand, within Psest_0411at 447.065 kb on - strand, within Psest_0411at 447.065 kb on - strand, within Psest_0411at 447.065 kb on - strand, within Psest_0411at 447.081 kb on + strand, within Psest_0411at 447.094 kb on + strand, within Psest_0411at 447.094 kb on + strand, within Psest_0411at 447.095 kb on - strand, within Psest_0411at 447.095 kb on - strand, within Psest_0411at 447.095 kb on - strand, within Psest_0411at 447.097 kb on + strand, within Psest_0411at 447.124 kb on + strand, within Psest_0411at 447.124 kb on + strand, within Psest_0411at 447.124 kb on + strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.152 kb on - strand, within Psest_0411at 447.637 kb on + strandat 447.637 kb on + strandat 447.637 kb on + strandat 447.637 kb on + strandat 447.637 kb on + strandat 447.637 kb on + strandat 447.638 kb on - strandat 447.640 kb on - strandat 447.814 kb on - strandat 447.900 kb on + strand, within Psest_0412at 447.901 kb on - strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.080 kb on + strand, within Psest_0412at 448.081 kb on - strand, within Psest_0412at 448.081 kb on - strand, within Psest_0412at 448.081 kb on - strand, within Psest_0412at 448.081 kb on - strand, within Psest_0412at 448.082 kb on + strand, within Psest_0412at 448.083 kb on - strand, within Psest_0412at 448.282 kb on + strand, within Psest_0412at 448.545 kb on + strand, within Psest_0412at 448.545 kb on + strand, within Psest_0412at 448.545 kb on + strand, within Psest_0412at 448.545 kb on + strand, within Psest_0412at 448.546 kb on - strand, within Psest_0412at 448.546 kb on - strand, within Psest_0412at 448.546 kb on - strand, within Psest_0412at 448.551 kb on + strand, within Psest_0412at 448.551 kb on + strand, within Psest_0412at 448.551 kb on + strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.552 kb on - strand, within Psest_0412at 448.613 kb on + strand, within Psest_0412at 448.614 kb on - strand, within Psest_0412at 448.859 kb on + strandat 449.196 kb on + strand, within Psest_0413at 449.196 kb on + strand, within Psest_0413at 449.196 kb on + strand, within Psest_0413at 449.196 kb on + strand, within Psest_0413at 449.197 kb on - strand, within Psest_0413at 449.197 kb on - strand, within Psest_0413at 449.197 kb on - strand, within Psest_0413at 449.197 kb on - strand, within Psest_0413at 449.197 kb on - strand, within Psest_0413at 449.341 kb on + strand, within Psest_0413at 449.341 kb on + strand, within Psest_0413at 449.341 kb on + strand, within Psest_0413at 449.342 kb on - strand, within Psest_0413at 449.342 kb on - strand, within Psest_0413at 449.461 kb on + strand, within Psest_0413at 449.462 kb on - strand, within Psest_0413at 449.462 kb on - strand, within Psest_0413at 449.721 kb on - strandat 449.721 kb on - strandat 449.721 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2
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446,791 + Psest_0411 0.18 -3.0
446,791 + Psest_0411 0.18 -0.9
446,791 + Psest_0411 0.18 -2.6
446,791 + Psest_0411 0.18 -1.1
446,791 + Psest_0411 0.18 +0.4
446,791 + Psest_0411 0.18 -2.5
446,792 - Psest_0411 0.18 -0.6
446,813 - Psest_0411 0.20 -4.1
446,813 - Psest_0411 0.20 -2.2
446,813 - Psest_0411 0.20 -0.6
446,950 + Psest_0411 0.33 -1.7
446,951 - Psest_0411 0.33 -1.4
446,951 - Psest_0411 0.33 -2.6
447,062 - Psest_0411 0.44 -1.3
447,064 + Psest_0411 0.44 -2.2
447,064 + Psest_0411 0.44 -3.4
447,064 + Psest_0411 0.44 -2.7
447,064 + Psest_0411 0.44 -3.7
447,064 + Psest_0411 0.44 -0.1
447,065 - Psest_0411 0.44 -1.8
447,065 - Psest_0411 0.44 -2.8
447,065 - Psest_0411 0.44 +0.2
447,065 - Psest_0411 0.44 -2.4
447,081 + Psest_0411 0.46 +0.4
447,094 + Psest_0411 0.47 -2.2
447,094 + Psest_0411 0.47 -1.7
447,095 - Psest_0411 0.47 -0.9
447,095 - Psest_0411 0.47 -1.9
447,095 - Psest_0411 0.47 -3.4
447,097 + Psest_0411 0.47 -3.7
447,124 + Psest_0411 0.50 -3.1
447,124 + Psest_0411 0.50 -2.6
447,124 + Psest_0411 0.50 -0.8
447,152 - Psest_0411 0.52 -3.4
447,152 - Psest_0411 0.52 -1.2
447,152 - Psest_0411 0.52 -2.7
447,152 - Psest_0411 0.52 -2.3
447,152 - Psest_0411 0.52 -0.1
447,152 - Psest_0411 0.52 -2.2
447,152 - Psest_0411 0.52 -0.6
447,637 + +1.2
447,637 + +4.0
447,637 + +3.2
447,637 + +0.4
447,637 + +0.4
447,637 + +0.6
447,638 - +0.9
447,640 - +1.3
447,814 - -1.5
447,900 + Psest_0412 0.12 -2.2
447,901 - Psest_0412 0.12 +1.4
448,080 + Psest_0412 0.27 -2.4
448,080 + Psest_0412 0.27 -0.4
448,080 + Psest_0412 0.27 -1.8
448,080 + Psest_0412 0.27 -2.5
448,080 + Psest_0412 0.27 -2.4
448,080 + Psest_0412 0.27 -0.5
448,080 + Psest_0412 0.27 +0.2
448,081 - Psest_0412 0.27 -0.8
448,081 - Psest_0412 0.27 -3.5
448,081 - Psest_0412 0.27 -2.0
448,081 - Psest_0412 0.27 -2.5
448,082 + Psest_0412 0.27 -2.5
448,083 - Psest_0412 0.27 -1.9
448,282 + Psest_0412 0.44 -1.9
448,545 + Psest_0412 0.66 -0.8
448,545 + Psest_0412 0.66 -1.7
448,545 + Psest_0412 0.66 +1.6
448,545 + Psest_0412 0.66 -1.9
448,546 - Psest_0412 0.66 +0.6
448,546 - Psest_0412 0.66 -0.1
448,546 - Psest_0412 0.66 -1.9
448,551 + Psest_0412 0.67 -2.8
448,551 + Psest_0412 0.67 +1.1
448,551 + Psest_0412 0.67 -1.4
448,552 - Psest_0412 0.67 -0.4
448,552 - Psest_0412 0.67 -1.7
448,552 - Psest_0412 0.67 -1.8
448,552 - Psest_0412 0.67 -0.8
448,552 - Psest_0412 0.67 -0.1
448,552 - Psest_0412 0.67 -1.1
448,552 - Psest_0412 0.67 -0.1
448,552 - Psest_0412 0.67 -2.8
448,552 - Psest_0412 0.67 -1.1
448,552 - Psest_0412 0.67 -2.1
448,552 - Psest_0412 0.67 -1.5
448,552 - Psest_0412 0.67 -2.3
448,552 - Psest_0412 0.67 -1.3
448,552 - Psest_0412 0.67 -1.4
448,552 - Psest_0412 0.67 -1.8
448,613 + Psest_0412 0.72 +1.1
448,614 - Psest_0412 0.72 -0.4
448,859 + -0.4
449,196 + Psest_0413 0.36 -0.6
449,196 + Psest_0413 0.36 -2.6
449,196 + Psest_0413 0.36 -2.0
449,196 + Psest_0413 0.36 -2.0
449,197 - Psest_0413 0.36 +0.3
449,197 - Psest_0413 0.36 -0.9
449,197 - Psest_0413 0.36 -2.5
449,197 - Psest_0413 0.36 -0.8
449,197 - Psest_0413 0.36 -0.9
449,341 + Psest_0413 0.56 -1.2
449,341 + Psest_0413 0.56 +0.4
449,341 + Psest_0413 0.56 -0.8
449,342 - Psest_0413 0.56 -0.6
449,342 - Psest_0413 0.56 +2.1
449,461 + Psest_0413 0.73 -1.6
449,462 - Psest_0413 0.73 -0.7
449,462 - Psest_0413 0.73 -0.7
449,721 - +0.1
449,721 - -3.3
449,721 - -3.1

Or see this region's nucleotide sequence