Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3824 and Psest_3825 are separated by 18 nucleotides Psest_3825 and Psest_3826 overlap by 4 nucleotides Psest_3826 and Psest_3827 are separated by 86 nucleotides
Psest_3824: Psest_3824 - Predicted membrane protein, at 3,995,080 to 3,995,697
_3824
Psest_3825: Psest_3825 - Beta-propeller domains of methanol dehydrogenase type, at 3,995,716 to 3,996,468
_3825
Psest_3826: Psest_3826 - Uncharacterized conserved protein, at 3,996,465 to 3,997,073
_3826
Psest_3827: Psest_3827 - Transcriptional regulator, at 3,997,160 to 3,998,080
_3827
Position (kb)
3996
3997
3998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3995.579 kb on + strand, within Psest_3824 at 3995.579 kb on + strand, within Psest_3824 at 3995.580 kb on - strand, within Psest_3824 at 3995.580 kb on - strand, within Psest_3824 at 3995.615 kb on - strand, within Psest_3824 at 3995.689 kb on + strand at 3995.689 kb on + strand at 3995.718 kb on - strand at 3995.718 kb on - strand at 3995.718 kb on - strand at 3996.544 kb on + strand, within Psest_3826 at 3996.544 kb on + strand, within Psest_3826 at 3996.544 kb on + strand, within Psest_3826 at 3996.544 kb on + strand, within Psest_3826 at 3996.544 kb on + strand, within Psest_3826 at 3996.545 kb on - strand, within Psest_3826 at 3996.545 kb on - strand, within Psest_3826 at 3996.545 kb on - strand, within Psest_3826 at 3996.545 kb on - strand at 3996.545 kb on - strand, within Psest_3826 at 3996.545 kb on - strand, within Psest_3826 at 3996.545 kb on - strand, within Psest_3826 at 3996.595 kb on + strand, within Psest_3826 at 3996.595 kb on + strand, within Psest_3826 at 3996.976 kb on + strand, within Psest_3826 at 3996.976 kb on + strand, within Psest_3826 at 3996.995 kb on + strand, within Psest_3826 at 3997.051 kb on + strand at 3997.067 kb on + strand at 3997.067 kb on + strand at 3997.068 kb on - strand at 3997.068 kb on - strand at 3997.068 kb on - strand at 3997.068 kb on - strand at 3997.068 kb on - strand at 3997.109 kb on + strand at 3997.109 kb on + strand at 3997.109 kb on + strand at 3997.128 kb on + strand at 3997.161 kb on + strand at 3997.362 kb on + strand, within Psest_3827 at 3997.362 kb on + strand, within Psest_3827 at 3997.362 kb on + strand, within Psest_3827 at 3997.362 kb on + strand, within Psest_3827 at 3997.434 kb on - strand, within Psest_3827 at 3997.567 kb on - strand, within Psest_3827 at 3997.567 kb on - strand, within Psest_3827 at 3997.887 kb on + strand, within Psest_3827
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 3,995,579 + Psest_3824 0.81 -1.2 3,995,579 + Psest_3824 0.81 -1.9 3,995,580 - Psest_3824 0.81 +2.0 3,995,580 - Psest_3824 0.81 +4.4 3,995,615 - Psest_3824 0.87 +0.9 3,995,689 + +1.7 3,995,689 + +0.4 3,995,718 - -2.3 3,995,718 - -2.1 3,995,718 - -0.1 3,996,544 + Psest_3826 0.13 -0.8 3,996,544 + Psest_3826 0.13 +2.6 3,996,544 + Psest_3826 0.13 -0.6 3,996,544 + Psest_3826 0.13 +0.4 3,996,544 + Psest_3826 0.13 +2.4 3,996,545 - Psest_3826 0.13 +2.7 3,996,545 - Psest_3826 0.13 +2.1 3,996,545 - Psest_3826 0.13 -0.2 3,996,545 - +0.2 3,996,545 - Psest_3826 0.13 -1.4 3,996,545 - Psest_3826 0.13 -0.6 3,996,545 - Psest_3826 0.13 +0.1 3,996,595 + Psest_3826 0.21 +1.3 3,996,595 + Psest_3826 0.21 +2.9 3,996,976 + Psest_3826 0.84 -0.3 3,996,976 + Psest_3826 0.84 +2.5 3,996,995 + Psest_3826 0.87 -2.3 3,997,051 + -0.8 3,997,067 + -3.0 3,997,067 + +1.0 3,997,068 - +0.6 3,997,068 - +2.6 3,997,068 - -0.7 3,997,068 - -0.0 3,997,068 - +0.6 3,997,109 + +0.6 3,997,109 + +2.8 3,997,109 + +1.4 3,997,128 + +1.6 3,997,161 + -0.6 3,997,362 + Psest_3827 0.22 -0.9 3,997,362 + Psest_3827 0.22 +0.9 3,997,362 + Psest_3827 0.22 +3.7 3,997,362 + Psest_3827 0.22 +0.2 3,997,434 - Psest_3827 0.30 -0.6 3,997,567 - Psest_3827 0.44 +0.2 3,997,567 - Psest_3827 0.44 +2.6 3,997,887 + Psest_3827 0.79 +1.1
Or see this region's nucleotide sequence