Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3653 and Psest_3654 are separated by 158 nucleotides Psest_3654 and Psest_3655 are separated by 12 nucleotides Psest_3655 and Psest_3656 are separated by 60 nucleotides
Psest_3653: Psest_3653 - 4-aminobutyrate aminotransferase, prokaryotic type, at 3,813,110 to 3,814,390
_3653
Psest_3654: Psest_3654 - succinate-semialdehyde dehydrogenase, at 3,814,549 to 3,816,015
_3654
Psest_3655: Psest_3655 - ABC-type spermidine/putrescine transport system, permease component II, at 3,816,028 to 3,816,855
_3655
Psest_3656: Psest_3656 - ABC-type spermidine/putrescine transport system, permease component I, at 3,816,916 to 3,818,169
_3656
Position (kb)
3814
3815
3816
3817 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3813.778 kb on + strand, within Psest_3653 at 3813.778 kb on + strand, within Psest_3653 at 3813.779 kb on - strand, within Psest_3653 at 3813.861 kb on + strand, within Psest_3653 at 3813.861 kb on + strand, within Psest_3653 at 3813.862 kb on - strand, within Psest_3653 at 3813.862 kb on - strand, within Psest_3653 at 3813.877 kb on - strand, within Psest_3653 at 3813.925 kb on - strand, within Psest_3653 at 3813.975 kb on + strand, within Psest_3653 at 3813.975 kb on + strand, within Psest_3653 at 3813.975 kb on + strand, within Psest_3653 at 3813.976 kb on - strand, within Psest_3653 at 3814.131 kb on + strand, within Psest_3653 at 3814.132 kb on - strand, within Psest_3653 at 3814.132 kb on - strand, within Psest_3653 at 3814.141 kb on - strand, within Psest_3653 at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.390 kb on + strand at 3814.391 kb on - strand at 3814.391 kb on - strand at 3814.391 kb on - strand at 3814.391 kb on - strand at 3814.459 kb on + strand at 3814.459 kb on + strand at 3814.459 kb on + strand at 3814.460 kb on - strand at 3814.460 kb on - strand at 3814.460 kb on - strand at 3814.498 kb on + strand at 3814.503 kb on + strand at 3814.504 kb on - strand at 3814.504 kb on - strand at 3814.519 kb on - strand at 3814.519 kb on - strand at 3814.519 kb on - strand at 3814.537 kb on - strand at 3814.571 kb on + strand at 3814.571 kb on + strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.572 kb on - strand at 3814.586 kb on + strand at 3814.586 kb on + strand at 3814.586 kb on + strand at 3814.586 kb on + strand at 3814.586 kb on + strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.587 kb on - strand at 3814.601 kb on + strand at 3814.745 kb on + strand, within Psest_3654 at 3814.752 kb on - strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.096 kb on + strand, within Psest_3654 at 3815.097 kb on - strand, within Psest_3654 at 3815.097 kb on - strand, within Psest_3654 at 3815.097 kb on - strand, within Psest_3654 at 3815.110 kb on + strand, within Psest_3654 at 3815.110 kb on + strand, within Psest_3654 at 3815.111 kb on - strand, within Psest_3654 at 3815.111 kb on - strand, within Psest_3654 at 3815.111 kb on - strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.114 kb on + strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.115 kb on - strand, within Psest_3654 at 3815.383 kb on - strand, within Psest_3654 at 3815.391 kb on + strand, within Psest_3654 at 3815.588 kb on - strand, within Psest_3654 at 3815.588 kb on - strand, within Psest_3654 at 3815.630 kb on + strand, within Psest_3654 at 3815.631 kb on - strand, within Psest_3654 at 3815.631 kb on - strand, within Psest_3654 at 3815.631 kb on - strand, within Psest_3654 at 3815.641 kb on - strand, within Psest_3654 at 3815.679 kb on - strand, within Psest_3654 at 3815.772 kb on - strand, within Psest_3654 at 3815.969 kb on + strand at 3815.969 kb on + strand at 3815.970 kb on - strand at 3816.021 kb on + strand at 3816.157 kb on + strand, within Psest_3655 at 3816.159 kb on + strand, within Psest_3655 at 3816.356 kb on + strand, within Psest_3655 at 3816.356 kb on + strand, within Psest_3655 at 3816.357 kb on - strand, within Psest_3655 at 3816.357 kb on - strand, within Psest_3655 at 3816.357 kb on - strand, within Psest_3655 at 3816.357 kb on - strand, within Psest_3655 at 3816.357 kb on - strand, within Psest_3655 at 3816.440 kb on + strand, within Psest_3655 at 3816.440 kb on + strand, within Psest_3655 at 3816.440 kb on + strand, within Psest_3655 at 3816.441 kb on - strand, within Psest_3655 at 3816.441 kb on - strand, within Psest_3655 at 3816.441 kb on - strand, within Psest_3655 at 3816.441 kb on - strand, within Psest_3655 at 3816.476 kb on + strand, within Psest_3655 at 3816.476 kb on + strand, within Psest_3655 at 3816.477 kb on - strand, within Psest_3655 at 3816.477 kb on - strand, within Psest_3655 at 3816.526 kb on + strand, within Psest_3655 at 3816.526 kb on + strand, within Psest_3655 at 3816.527 kb on - strand, within Psest_3655 at 3816.527 kb on - strand, within Psest_3655 at 3816.683 kb on + strand, within Psest_3655 at 3816.684 kb on - strand, within Psest_3655 at 3816.714 kb on - strand, within Psest_3655 at 3816.813 kb on - strand at 3816.815 kb on + strand at 3816.815 kb on + strand at 3816.816 kb on - strand at 3816.816 kb on - strand at 3816.842 kb on + strand at 3816.842 kb on + strand at 3816.842 kb on + strand at 3816.843 kb on - strand at 3816.843 kb on - strand at 3816.843 kb on - strand at 3816.843 kb on - strand at 3816.956 kb on + strand at 3816.956 kb on + strand at 3816.956 kb on + strand at 3816.956 kb on + strand at 3816.956 kb on + strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.957 kb on - strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.965 kb on + strand at 3816.966 kb on - strand at 3816.966 kb on - strand at 3816.966 kb on - strand at 3816.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 3,813,778 + Psest_3653 0.52 +3.3 3,813,778 + Psest_3653 0.52 +1.7 3,813,779 - Psest_3653 0.52 +2.0 3,813,861 + Psest_3653 0.59 +0.2 3,813,861 + Psest_3653 0.59 +0.0 3,813,862 - Psest_3653 0.59 +3.4 3,813,862 - Psest_3653 0.59 +0.5 3,813,877 - Psest_3653 0.60 +0.1 3,813,925 - Psest_3653 0.64 -2.2 3,813,975 + Psest_3653 0.68 +2.0 3,813,975 + Psest_3653 0.68 +0.8 3,813,975 + Psest_3653 0.68 +0.8 3,813,976 - Psest_3653 0.68 -0.4 3,814,131 + Psest_3653 0.80 -2.6 3,814,132 - Psest_3653 0.80 +1.2 3,814,132 - Psest_3653 0.80 -1.5 3,814,141 - Psest_3653 0.80 +0.5 3,814,390 + -0.3 3,814,390 + -0.4 3,814,390 + +0.8 3,814,390 + +3.4 3,814,390 + +2.2 3,814,390 + +1.2 3,814,390 + +0.8 3,814,390 + +0.9 3,814,391 - +0.7 3,814,391 - +0.6 3,814,391 - -1.1 3,814,391 - +0.7 3,814,459 + +3.4 3,814,459 + +1.3 3,814,459 + +0.1 3,814,460 - -0.4 3,814,460 - +1.2 3,814,460 - -0.7 3,814,498 + +2.2 3,814,503 + +0.9 3,814,504 - +1.3 3,814,504 - +0.5 3,814,519 - +2.2 3,814,519 - +0.0 3,814,519 - -0.8 3,814,537 - +1.0 3,814,571 + -0.0 3,814,571 + +1.0 3,814,572 - +0.0 3,814,572 - +0.7 3,814,572 - +0.2 3,814,572 - +0.1 3,814,572 - +0.6 3,814,572 - +1.7 3,814,572 - -0.3 3,814,572 - +2.9 3,814,586 + +0.5 3,814,586 + +0.4 3,814,586 + -1.2 3,814,586 + +0.5 3,814,586 + -1.9 3,814,587 - +1.4 3,814,587 - -0.5 3,814,587 - -1.4 3,814,587 - -1.3 3,814,587 - +1.2 3,814,587 - -1.5 3,814,587 - +0.7 3,814,601 + +0.3 3,814,745 + Psest_3654 0.13 +0.7 3,814,752 - Psest_3654 0.14 -2.0 3,815,096 + Psest_3654 0.37 -1.4 3,815,096 + Psest_3654 0.37 -1.8 3,815,096 + Psest_3654 0.37 -0.0 3,815,096 + Psest_3654 0.37 -0.5 3,815,096 + Psest_3654 0.37 +1.2 3,815,096 + Psest_3654 0.37 -0.9 3,815,097 - Psest_3654 0.37 -2.6 3,815,097 - Psest_3654 0.37 -2.1 3,815,097 - Psest_3654 0.37 +0.9 3,815,110 + Psest_3654 0.38 -1.8 3,815,110 + Psest_3654 0.38 -2.9 3,815,111 - Psest_3654 0.38 +0.5 3,815,111 - Psest_3654 0.38 -2.1 3,815,111 - Psest_3654 0.38 +0.7 3,815,114 + Psest_3654 0.39 +0.8 3,815,114 + Psest_3654 0.39 -0.3 3,815,114 + Psest_3654 0.39 -0.8 3,815,114 + Psest_3654 0.39 -0.3 3,815,114 + Psest_3654 0.39 -2.9 3,815,114 + Psest_3654 0.39 +0.3 3,815,115 - Psest_3654 0.39 -0.8 3,815,115 - Psest_3654 0.39 -0.9 3,815,115 - Psest_3654 0.39 +0.9 3,815,115 - Psest_3654 0.39 -2.1 3,815,115 - Psest_3654 0.39 +0.1 3,815,115 - Psest_3654 0.39 +0.8 3,815,115 - Psest_3654 0.39 +1.8 3,815,115 - Psest_3654 0.39 -0.3 3,815,115 - Psest_3654 0.39 -1.4 3,815,383 - Psest_3654 0.57 +2.2 3,815,391 + Psest_3654 0.57 -1.2 3,815,588 - Psest_3654 0.71 -0.9 3,815,588 - Psest_3654 0.71 -0.9 3,815,630 + Psest_3654 0.74 -1.5 3,815,631 - Psest_3654 0.74 +0.0 3,815,631 - Psest_3654 0.74 -2.8 3,815,631 - Psest_3654 0.74 -1.5 3,815,641 - Psest_3654 0.74 +1.5 3,815,679 - Psest_3654 0.77 -0.9 3,815,772 - Psest_3654 0.83 -0.6 3,815,969 + +1.1 3,815,969 + -1.9 3,815,970 - +2.1 3,816,021 + +2.3 3,816,157 + Psest_3655 0.16 -1.9 3,816,159 + Psest_3655 0.16 +0.5 3,816,356 + Psest_3655 0.40 +2.1 3,816,356 + Psest_3655 0.40 -1.5 3,816,357 - Psest_3655 0.40 +1.5 3,816,357 - Psest_3655 0.40 -0.7 3,816,357 - Psest_3655 0.40 +1.1 3,816,357 - Psest_3655 0.40 +3.3 3,816,357 - Psest_3655 0.40 -1.3 3,816,440 + Psest_3655 0.50 -0.5 3,816,440 + Psest_3655 0.50 -1.1 3,816,440 + Psest_3655 0.50 -0.4 3,816,441 - Psest_3655 0.50 +1.1 3,816,441 - Psest_3655 0.50 +0.0 3,816,441 - Psest_3655 0.50 -1.9 3,816,441 - Psest_3655 0.50 -1.0 3,816,476 + Psest_3655 0.54 -0.8 3,816,476 + Psest_3655 0.54 -1.2 3,816,477 - Psest_3655 0.54 -0.0 3,816,477 - Psest_3655 0.54 -0.4 3,816,526 + Psest_3655 0.60 -1.6 3,816,526 + Psest_3655 0.60 -2.2 3,816,527 - Psest_3655 0.60 -1.5 3,816,527 - Psest_3655 0.60 +0.2 3,816,683 + Psest_3655 0.79 -1.6 3,816,684 - Psest_3655 0.79 -0.5 3,816,714 - Psest_3655 0.83 +0.7 3,816,813 - +0.2 3,816,815 + -1.3 3,816,815 + -0.6 3,816,816 - +0.4 3,816,816 - -1.7 3,816,842 + +0.1 3,816,842 + -2.2 3,816,842 + -1.7 3,816,843 - -0.2 3,816,843 - +0.2 3,816,843 - +0.7 3,816,843 - +2.2 3,816,956 + -2.5 3,816,956 + -0.6 3,816,956 + +0.6 3,816,956 + -0.8 3,816,956 + -2.2 3,816,957 - +1.3 3,816,957 - +0.4 3,816,957 - -0.2 3,816,957 - +2.0 3,816,957 - +0.9 3,816,957 - -1.2 3,816,957 - -0.6 3,816,957 - -0.6 3,816,957 - -0.5 3,816,957 - -0.1 3,816,965 + -2.6 3,816,965 + -0.4 3,816,965 + +1.0 3,816,965 + -3.0 3,816,965 + +0.8 3,816,965 + -0.8 3,816,965 + -0.9 3,816,966 - -1.7 3,816,966 - -1.5 3,816,966 - +0.1 3,816,966 - -0.9
Or see this region's nucleotide sequence