Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3181 and Psest_3182 overlap by 4 nucleotides Psest_3182 and Psest_3183 overlap by 14 nucleotides Psest_3183 and Psest_3184 overlap by 4 nucleotides
Psest_3181: Psest_3181 - Predicted membrane protein/domain, at 3,353,321 to 3,354,034
_3181
Psest_3182: Psest_3182 - Uncharacterized membrane protein, at 3,354,031 to 3,355,011
_3182
Psest_3183: Psest_3183 - hypothetical protein, at 3,354,998 to 3,356,551
_3183
Psest_3184: Psest_3184 - hypothetical protein, at 3,356,548 to 3,357,720
_3184
Position (kb)
3354
3355
3356
3357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3354.196 kb on + strand, within Psest_3182 at 3354.340 kb on + strand, within Psest_3182 at 3354.340 kb on + strand, within Psest_3182 at 3354.341 kb on - strand, within Psest_3182 at 3354.341 kb on - strand, within Psest_3182 at 3354.341 kb on - strand, within Psest_3182 at 3354.341 kb on - strand, within Psest_3182 at 3354.368 kb on - strand, within Psest_3182 at 3354.400 kb on + strand, within Psest_3182 at 3354.400 kb on + strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.401 kb on - strand, within Psest_3182 at 3354.421 kb on + strand, within Psest_3182 at 3354.421 kb on + strand, within Psest_3182 at 3354.422 kb on - strand, within Psest_3182 at 3354.422 kb on - strand, within Psest_3182 at 3354.466 kb on + strand, within Psest_3182 at 3354.466 kb on + strand, within Psest_3182 at 3354.467 kb on - strand, within Psest_3182 at 3354.467 kb on - strand, within Psest_3182 at 3354.467 kb on - strand, within Psest_3182 at 3354.467 kb on - strand, within Psest_3182 at 3354.467 kb on - strand, within Psest_3182 at 3354.539 kb on - strand, within Psest_3182 at 3354.541 kb on + strand, within Psest_3182 at 3354.541 kb on + strand, within Psest_3182 at 3354.541 kb on + strand, within Psest_3182 at 3354.541 kb on + strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.542 kb on - strand, within Psest_3182 at 3354.836 kb on + strand, within Psest_3182 at 3354.836 kb on + strand, within Psest_3182 at 3354.836 kb on + strand, within Psest_3182 at 3354.836 kb on + strand, within Psest_3182 at 3354.836 kb on + strand, within Psest_3182 at 3354.837 kb on - strand, within Psest_3182 at 3354.837 kb on - strand, within Psest_3182 at 3354.837 kb on - strand, within Psest_3182 at 3354.837 kb on - strand, within Psest_3182 at 3354.932 kb on - strand at 3354.932 kb on - strand at 3354.932 kb on - strand at 3354.932 kb on - strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.938 kb on + strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.939 kb on - strand at 3354.970 kb on + strand at 3354.970 kb on + strand at 3354.970 kb on + strand at 3355.176 kb on + strand, within Psest_3183 at 3355.177 kb on - strand, within Psest_3183 at 3355.206 kb on - strand, within Psest_3183 at 3355.206 kb on - strand, within Psest_3183 at 3355.206 kb on - strand, within Psest_3183 at 3355.226 kb on - strand, within Psest_3183 at 3355.512 kb on - strand, within Psest_3183 at 3355.600 kb on - strand, within Psest_3183 at 3355.600 kb on - strand, within Psest_3183 at 3355.613 kb on + strand, within Psest_3183 at 3355.613 kb on + strand, within Psest_3183 at 3355.613 kb on + strand, within Psest_3183 at 3355.613 kb on + strand, within Psest_3183 at 3355.613 kb on + strand, within Psest_3183 at 3355.646 kb on + strand, within Psest_3183 at 3355.646 kb on + strand, within Psest_3183 at 3355.647 kb on - strand, within Psest_3183 at 3356.167 kb on - strand, within Psest_3183 at 3356.167 kb on - strand, within Psest_3183 at 3356.248 kb on + strand, within Psest_3183 at 3356.309 kb on + strand, within Psest_3183 at 3356.466 kb on - strand at 3356.639 kb on - strand at 3356.706 kb on - strand, within Psest_3184 at 3357.034 kb on + strand, within Psest_3184 at 3357.035 kb on - strand, within Psest_3184 at 3357.227 kb on - strand, within Psest_3184 at 3357.250 kb on + strand, within Psest_3184 at 3357.250 kb on + strand, within Psest_3184 at 3357.251 kb on - strand, within Psest_3184 at 3357.326 kb on - strand, within Psest_3184 at 3357.362 kb on + strand, within Psest_3184 at 3357.362 kb on + strand, within Psest_3184 at 3357.363 kb on - strand, within Psest_3184 at 3357.363 kb on - strand, within Psest_3184 at 3357.363 kb on - strand, within Psest_3184 at 3357.363 kb on - strand, within Psest_3184 at 3357.449 kb on + strand, within Psest_3184 at 3357.450 kb on - strand, within Psest_3184
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 3,354,196 + Psest_3182 0.17 +0.3 3,354,340 + Psest_3182 0.31 -1.6 3,354,340 + Psest_3182 0.31 -1.8 3,354,341 - Psest_3182 0.32 -1.0 3,354,341 - Psest_3182 0.32 +2.6 3,354,341 - Psest_3182 0.32 -0.0 3,354,341 - Psest_3182 0.32 +0.2 3,354,368 - Psest_3182 0.34 -0.1 3,354,400 + Psest_3182 0.38 -1.0 3,354,400 + Psest_3182 0.38 +1.2 3,354,401 - Psest_3182 0.38 -0.7 3,354,401 - Psest_3182 0.38 -1.5 3,354,401 - Psest_3182 0.38 +0.7 3,354,401 - Psest_3182 0.38 -0.2 3,354,401 - Psest_3182 0.38 -2.7 3,354,401 - Psest_3182 0.38 -1.0 3,354,421 + Psest_3182 0.40 +1.0 3,354,421 + Psest_3182 0.40 +3.9 3,354,422 - Psest_3182 0.40 +1.1 3,354,422 - Psest_3182 0.40 -2.1 3,354,466 + Psest_3182 0.44 -0.4 3,354,466 + Psest_3182 0.44 -1.4 3,354,467 - Psest_3182 0.44 +0.2 3,354,467 - Psest_3182 0.44 -1.4 3,354,467 - Psest_3182 0.44 +1.8 3,354,467 - Psest_3182 0.44 +0.9 3,354,467 - Psest_3182 0.44 +2.7 3,354,539 - Psest_3182 0.52 -2.4 3,354,541 + Psest_3182 0.52 -2.0 3,354,541 + Psest_3182 0.52 -0.9 3,354,541 + Psest_3182 0.52 +0.9 3,354,541 + Psest_3182 0.52 +0.3 3,354,542 - Psest_3182 0.52 +0.9 3,354,542 - Psest_3182 0.52 +0.8 3,354,542 - Psest_3182 0.52 -0.4 3,354,542 - Psest_3182 0.52 -0.1 3,354,542 - Psest_3182 0.52 +1.0 3,354,542 - Psest_3182 0.52 -1.6 3,354,542 - Psest_3182 0.52 -0.2 3,354,542 - Psest_3182 0.52 -0.1 3,354,542 - Psest_3182 0.52 -1.9 3,354,542 - Psest_3182 0.52 -0.1 3,354,542 - Psest_3182 0.52 +0.8 3,354,542 - Psest_3182 0.52 -0.3 3,354,542 - Psest_3182 0.52 +0.2 3,354,542 - Psest_3182 0.52 +0.8 3,354,542 - Psest_3182 0.52 -1.0 3,354,542 - Psest_3182 0.52 -1.0 3,354,542 - Psest_3182 0.52 -0.2 3,354,542 - Psest_3182 0.52 +3.8 3,354,836 + Psest_3182 0.82 +0.0 3,354,836 + Psest_3182 0.82 -1.2 3,354,836 + Psest_3182 0.82 +1.2 3,354,836 + Psest_3182 0.82 +0.2 3,354,836 + Psest_3182 0.82 +1.2 3,354,837 - Psest_3182 0.82 -1.6 3,354,837 - Psest_3182 0.82 +0.3 3,354,837 - Psest_3182 0.82 -0.5 3,354,837 - Psest_3182 0.82 -0.6 3,354,932 - +2.2 3,354,932 - +1.8 3,354,932 - +0.2 3,354,932 - +1.3 3,354,938 + +1.2 3,354,938 + -1.6 3,354,938 + -1.1 3,354,938 + -1.6 3,354,938 + -1.7 3,354,938 + -1.1 3,354,938 + -0.6 3,354,939 - +0.6 3,354,939 - -0.4 3,354,939 - +0.1 3,354,939 - +1.8 3,354,939 - +0.3 3,354,939 - +0.6 3,354,939 - +0.6 3,354,939 - +1.9 3,354,970 + -0.8 3,354,970 + -1.2 3,354,970 + -0.1 3,355,176 + Psest_3183 0.11 -2.2 3,355,177 - Psest_3183 0.12 -1.8 3,355,206 - Psest_3183 0.13 +0.3 3,355,206 - Psest_3183 0.13 -1.1 3,355,206 - Psest_3183 0.13 +0.2 3,355,226 - Psest_3183 0.15 -1.4 3,355,512 - Psest_3183 0.33 -2.5 3,355,600 - Psest_3183 0.39 +0.2 3,355,600 - Psest_3183 0.39 -1.0 3,355,613 + Psest_3183 0.40 -1.5 3,355,613 + Psest_3183 0.40 -0.1 3,355,613 + Psest_3183 0.40 +1.0 3,355,613 + Psest_3183 0.40 -3.6 3,355,613 + Psest_3183 0.40 +0.5 3,355,646 + Psest_3183 0.42 -1.8 3,355,646 + Psest_3183 0.42 -1.0 3,355,647 - Psest_3183 0.42 +0.5 3,356,167 - Psest_3183 0.75 -2.1 3,356,167 - Psest_3183 0.75 +0.2 3,356,248 + Psest_3183 0.80 -0.2 3,356,309 + Psest_3183 0.84 +0.4 3,356,466 - +0.4 3,356,639 - +0.0 3,356,706 - Psest_3184 0.13 -3.0 3,357,034 + Psest_3184 0.41 -2.4 3,357,035 - Psest_3184 0.42 -1.7 3,357,227 - Psest_3184 0.58 +3.0 3,357,250 + Psest_3184 0.60 +0.2 3,357,250 + Psest_3184 0.60 -0.6 3,357,251 - Psest_3184 0.60 +1.1 3,357,326 - Psest_3184 0.66 -0.0 3,357,362 + Psest_3184 0.69 +1.8 3,357,362 + Psest_3184 0.69 +1.5 3,357,363 - Psest_3184 0.69 -0.7 3,357,363 - Psest_3184 0.69 +1.1 3,357,363 - Psest_3184 0.69 +0.9 3,357,363 - Psest_3184 0.69 -0.6 3,357,449 + Psest_3184 0.77 -0.0 3,357,450 - Psest_3184 0.77 +0.4
Or see this region's nucleotide sequence