Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0309 and Psest_0310 are separated by 14 nucleotides Psest_0310 and Psest_0311 overlap by 4 nucleotides
Psest_0309: Psest_0309 - RND family efflux transporter, MFP subunit, at 328,340 to 329,482
_0309
Psest_0310: Psest_0310 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 329,497 to 332,634
_0310
Psest_0311: Psest_0311 - efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family, at 332,631 to 334,055
_0311
Position (kb) 329
330
331
332
333 Strain fitness (log2 ratio) -2
-1
0
1
2 at 328.736 kb on + strand, within Psest_0309 at 328.926 kb on - strand, within Psest_0309 at 329.162 kb on - strand, within Psest_0309 at 329.491 kb on + strand at 329.491 kb on + strand at 329.491 kb on + strand at 329.965 kb on + strand, within Psest_0310 at 330.104 kb on + strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.413 kb on + strand, within Psest_0310 at 330.414 kb on - strand, within Psest_0310 at 330.475 kb on + strand, within Psest_0310 at 330.563 kb on - strand, within Psest_0310 at 331.126 kb on + strand, within Psest_0310 at 331.126 kb on + strand, within Psest_0310 at 331.858 kb on + strand, within Psest_0310 at 332.164 kb on + strand, within Psest_0310 at 332.564 kb on - strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.748 kb on + strand at 333.015 kb on + strand, within Psest_0311 at 333.016 kb on - strand, within Psest_0311 at 333.097 kb on - strand, within Psest_0311 at 333.222 kb on + strand, within Psest_0311 at 333.223 kb on - strand, within Psest_0311 at 333.337 kb on - strand, within Psest_0311 at 333.472 kb on + strand, within Psest_0311 at 333.473 kb on - strand, within Psest_0311 at 333.473 kb on - strand, within Psest_0311
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 328,736 + Psest_0309 0.35 -0.1 328,926 - Psest_0309 0.51 -0.8 329,162 - Psest_0309 0.72 +2.2 329,491 + +2.2 329,491 + -1.3 329,491 + -2.3 329,965 + Psest_0310 0.15 +2.2 330,104 + Psest_0310 0.19 -0.6 330,105 - Psest_0310 0.19 -1.0 330,105 - Psest_0310 0.19 +0.2 330,105 - Psest_0310 0.19 +2.2 330,105 - Psest_0310 0.19 -0.1 330,105 - Psest_0310 0.19 +0.2 330,413 + Psest_0310 0.29 +0.6 330,414 - Psest_0310 0.29 +2.2 330,475 + Psest_0310 0.31 +0.6 330,563 - Psest_0310 0.34 -0.8 331,126 + Psest_0310 0.52 +1.2 331,126 + Psest_0310 0.52 +1.2 331,858 + Psest_0310 0.75 -0.4 332,164 + Psest_0310 0.85 +0.2 332,564 - +2.2 332,577 + +2.4 332,577 + -1.2 332,577 + +0.3 332,577 + -2.2 332,748 + -0.4 333,015 + Psest_0311 0.27 -0.6 333,016 - Psest_0311 0.27 -2.0 333,097 - Psest_0311 0.33 -1.5 333,222 + Psest_0311 0.41 -0.1 333,223 - Psest_0311 0.42 +2.2 333,337 - Psest_0311 0.50 -1.5 333,472 + Psest_0311 0.59 -0.8 333,473 - Psest_0311 0.59 -1.0 333,473 - Psest_0311 0.59 -0.4
Or see this region's nucleotide sequence