Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2946 and Psest_2947 overlap by 4 nucleotides Psest_2947 and Psest_2948 are separated by 61 nucleotides Psest_2948 and Psest_2949 are separated by 119 nucleotides
Psest_2946: Psest_2946 - septum site-determining protein MinD, at 3,143,335 to 3,144,150
_2946
Psest_2947: Psest_2947 - cell division topological specificity factor MinE, at 3,144,147 to 3,144,404
_2947
Psest_2948: Psest_2948 - pseudouridine synthase, RluA family, at 3,144,466 to 3,145,101
_2948
Psest_2949: Psest_2949 - Aspartyl aminopeptidase, at 3,145,221 to 3,146,510
_2949
Position (kb)
3144
3145
3146 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3143.506 kb on + strand, within Psest_2946 at 3143.507 kb on - strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.677 kb on + strand, within Psest_2946 at 3143.678 kb on - strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.731 kb on + strand, within Psest_2946 at 3143.732 kb on - strand, within Psest_2946 at 3143.756 kb on + strand, within Psest_2946 at 3143.867 kb on + strand, within Psest_2946 at 3143.878 kb on + strand, within Psest_2946 at 3143.879 kb on - strand, within Psest_2946 at 3144.047 kb on - strand, within Psest_2946 at 3144.309 kb on + strand, within Psest_2947 at 3144.310 kb on - strand, within Psest_2947 at 3144.420 kb on - strand at 3144.475 kb on - strand at 3144.655 kb on - strand, within Psest_2948 at 3144.655 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.731 kb on - strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.836 kb on + strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.837 kb on - strand, within Psest_2948 at 3144.982 kb on + strand, within Psest_2948 at 3144.983 kb on - strand, within Psest_2948 at 3144.983 kb on - strand, within Psest_2948 at 3145.099 kb on + strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.100 kb on - strand at 3145.102 kb on + strand at 3145.167 kb on - strand at 3145.167 kb on - strand at 3145.186 kb on + strand at 3145.187 kb on - strand at 3145.187 kb on - strand at 3145.187 kb on - strand at 3145.188 kb on + strand at 3145.188 kb on + strand at 3145.189 kb on - strand at 3145.189 kb on - strand at 3145.189 kb on - strand at 3145.189 kb on - strand at 3145.189 kb on - strand at 3145.377 kb on + strand, within Psest_2949 at 3145.377 kb on + strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.378 kb on - strand, within Psest_2949 at 3145.466 kb on + strand, within Psest_2949 at 3145.545 kb on + strand, within Psest_2949 at 3145.545 kb on + strand, within Psest_2949 at 3145.546 kb on - strand, within Psest_2949 at 3145.546 kb on - strand, within Psest_2949 at 3145.609 kb on - strand, within Psest_2949 at 3145.609 kb on - strand, within Psest_2949 at 3145.609 kb on - strand, within Psest_2949 at 3145.739 kb on - strand, within Psest_2949 at 3145.863 kb on + strand, within Psest_2949
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 3,143,506 + Psest_2946 0.21 -0.4 3,143,507 - Psest_2946 0.21 +0.2 3,143,677 + Psest_2946 0.42 -0.6 3,143,677 + Psest_2946 0.42 -0.4 3,143,677 + Psest_2946 0.42 +1.9 3,143,678 - Psest_2946 0.42 +0.6 3,143,731 + Psest_2946 0.49 +0.9 3,143,731 + Psest_2946 0.49 +3.5 3,143,731 + Psest_2946 0.49 +0.2 3,143,731 + Psest_2946 0.49 +0.5 3,143,731 + Psest_2946 0.49 +0.6 3,143,731 + Psest_2946 0.49 -0.4 3,143,731 + Psest_2946 0.49 -0.4 3,143,732 - Psest_2946 0.49 +0.6 3,143,756 + Psest_2946 0.52 +0.6 3,143,867 + Psest_2946 0.65 -0.1 3,143,878 + Psest_2946 0.67 +0.0 3,143,879 - Psest_2946 0.67 +0.2 3,144,047 - Psest_2946 0.87 -0.6 3,144,309 + Psest_2947 0.63 +0.7 3,144,310 - Psest_2947 0.63 +0.6 3,144,420 - +0.0 3,144,475 - +0.2 3,144,655 - Psest_2948 0.30 -1.4 3,144,655 - Psest_2948 0.30 -0.8 3,144,731 - Psest_2948 0.42 -1.8 3,144,731 - Psest_2948 0.42 -2.0 3,144,731 - Psest_2948 0.42 -1.1 3,144,836 + Psest_2948 0.58 -2.5 3,144,836 + Psest_2948 0.58 -0.9 3,144,836 + Psest_2948 0.58 +0.4 3,144,836 + Psest_2948 0.58 -0.5 3,144,836 + Psest_2948 0.58 -0.4 3,144,836 + Psest_2948 0.58 +0.8 3,144,837 - Psest_2948 0.58 -0.8 3,144,837 - Psest_2948 0.58 -0.6 3,144,837 - Psest_2948 0.58 -0.6 3,144,837 - Psest_2948 0.58 -0.3 3,144,837 - Psest_2948 0.58 +2.0 3,144,837 - Psest_2948 0.58 -0.4 3,144,982 + Psest_2948 0.81 +0.2 3,144,983 - Psest_2948 0.81 +0.8 3,144,983 - Psest_2948 0.81 +0.7 3,145,099 + +0.3 3,145,100 - +0.1 3,145,100 - +1.2 3,145,100 - -0.4 3,145,100 - -0.5 3,145,100 - +1.2 3,145,100 - -1.0 3,145,100 - +0.7 3,145,100 - -1.8 3,145,100 - -1.5 3,145,102 + -0.9 3,145,167 - -0.1 3,145,167 - +1.4 3,145,186 + +1.6 3,145,187 - -1.7 3,145,187 - -0.2 3,145,187 - -1.2 3,145,188 + -0.1 3,145,188 + -1.0 3,145,189 - -1.2 3,145,189 - +0.8 3,145,189 - -0.9 3,145,189 - +0.9 3,145,189 - -1.6 3,145,377 + Psest_2949 0.12 +0.1 3,145,377 + Psest_2949 0.12 +0.6 3,145,378 - Psest_2949 0.12 +0.2 3,145,378 - Psest_2949 0.12 +0.0 3,145,378 - Psest_2949 0.12 +1.2 3,145,378 - Psest_2949 0.12 +0.0 3,145,378 - Psest_2949 0.12 -0.3 3,145,378 - Psest_2949 0.12 +0.5 3,145,466 + Psest_2949 0.19 -0.4 3,145,545 + Psest_2949 0.25 +0.6 3,145,545 + Psest_2949 0.25 +0.2 3,145,546 - Psest_2949 0.25 -0.3 3,145,546 - Psest_2949 0.25 -1.8 3,145,609 - Psest_2949 0.30 -0.1 3,145,609 - Psest_2949 0.30 -1.6 3,145,609 - Psest_2949 0.30 +1.2 3,145,739 - Psest_2949 0.40 +0.5 3,145,863 + Psest_2949 0.50 +1.4
Or see this region's nucleotide sequence