Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2627 and Psest_2628 are separated by 12 nucleotides Psest_2628 and Psest_2629 overlap by 1 nucleotides Psest_2629 and Psest_2630 are separated by 184 nucleotides Psest_2630 and Psest_2631 are separated by 2 nucleotides
Psest_2627: Psest_2627 - hypothetical protein, at 2,809,257 to 2,810,576
_2627
Psest_2628: Psest_2628 - Flp pilus assembly protein, protease CpaA, at 2,810,589 to 2,811,095
_2628
Psest_2629: Psest_2629 - Flp pilus assembly protein, pilin Flp, at 2,811,095 to 2,811,295
_2629
Psest_2630: Psest_2630 - hypothetical protein, at 2,811,480 to 2,811,641
_2630
Psest_2631: Psest_2631 - AraC-type DNA-binding domain-containing proteins, at 2,811,644 to 2,812,516
_2631
Position (kb)
2811
2812 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2810.140 kb on + strand, within Psest_2627 at 2810.243 kb on - strand, within Psest_2627 at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.695 kb on + strand, within Psest_2628 at 2810.723 kb on - strand, within Psest_2628 at 2810.844 kb on + strand, within Psest_2628 at 2810.845 kb on - strand, within Psest_2628 at 2811.069 kb on + strand at 2811.069 kb on + strand at 2811.070 kb on - strand at 2811.070 kb on - strand at 2811.070 kb on - strand at 2811.072 kb on + strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.079 kb on - strand at 2811.096 kb on + strand at 2811.156 kb on - strand, within Psest_2629 at 2811.156 kb on - strand, within Psest_2629 at 2811.167 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.189 kb on - strand, within Psest_2629 at 2811.222 kb on + strand, within Psest_2629 at 2811.337 kb on + strand at 2811.384 kb on + strand at 2811.384 kb on + strand at 2811.385 kb on - strand at 2811.385 kb on - strand at 2811.385 kb on - strand at 2811.451 kb on + strand at 2811.451 kb on + strand at 2811.451 kb on + strand at 2811.535 kb on + strand, within Psest_2630 at 2811.535 kb on + strand, within Psest_2630 at 2811.535 kb on + strand, within Psest_2630 at 2811.536 kb on - strand, within Psest_2630 at 2811.541 kb on - strand, within Psest_2630 at 2811.566 kb on - strand, within Psest_2630 at 2811.582 kb on - strand, within Psest_2630 at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.640 kb on - strand at 2811.642 kb on + strand at 2811.642 kb on + strand at 2811.643 kb on - strand at 2811.652 kb on + strand at 2811.653 kb on - strand at 2811.706 kb on + strand at 2811.790 kb on - strand, within Psest_2631 at 2811.790 kb on - strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2812.254 kb on - strand, within Psest_2631 at 2812.265 kb on + strand, within Psest_2631 at 2812.266 kb on - strand, within Psest_2631
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 2,810,140 + Psest_2627 0.67 +0.3 2,810,243 - Psest_2627 0.75 -1.6 2,810,568 + -1.3 2,810,568 + +0.8 2,810,568 + +0.3 2,810,568 + +0.1 2,810,568 + -1.2 2,810,568 + -0.2 2,810,569 - -0.1 2,810,569 - +0.4 2,810,569 - +4.0 2,810,569 - -0.2 2,810,569 - +0.3 2,810,569 - +0.8 2,810,569 - +3.1 2,810,569 - -0.7 2,810,695 + Psest_2628 0.21 -1.4 2,810,723 - Psest_2628 0.26 -1.5 2,810,844 + Psest_2628 0.50 +2.0 2,810,845 - Psest_2628 0.50 -0.2 2,811,069 + -1.1 2,811,069 + +1.2 2,811,070 - -0.5 2,811,070 - +0.3 2,811,070 - -0.1 2,811,072 + +1.1 2,811,073 - +0.1 2,811,073 - -2.4 2,811,073 - +1.1 2,811,073 - +0.4 2,811,073 - +0.1 2,811,079 - -1.6 2,811,096 + -0.2 2,811,156 - Psest_2629 0.30 -0.3 2,811,156 - Psest_2629 0.30 -1.7 2,811,167 + Psest_2629 0.36 -0.6 2,811,188 + Psest_2629 0.46 -0.7 2,811,188 + Psest_2629 0.46 -1.5 2,811,188 + Psest_2629 0.46 +0.3 2,811,189 - Psest_2629 0.47 -1.2 2,811,222 + Psest_2629 0.63 -1.2 2,811,337 + +1.0 2,811,384 + +0.4 2,811,384 + -0.2 2,811,385 - -1.8 2,811,385 - -1.2 2,811,385 - -1.5 2,811,451 + -1.4 2,811,451 + +0.7 2,811,451 + +0.7 2,811,535 + Psest_2630 0.34 -0.3 2,811,535 + Psest_2630 0.34 +0.1 2,811,535 + Psest_2630 0.34 -2.7 2,811,536 - Psest_2630 0.35 -2.5 2,811,541 - Psest_2630 0.38 -0.5 2,811,566 - Psest_2630 0.53 +0.5 2,811,582 - Psest_2630 0.63 -0.4 2,811,639 + -0.1 2,811,639 + +1.9 2,811,639 + -2.3 2,811,639 + +0.1 2,811,640 - -1.3 2,811,642 + +0.5 2,811,642 + +1.5 2,811,643 - -0.2 2,811,652 + -0.7 2,811,653 - +3.7 2,811,706 + -0.5 2,811,790 - Psest_2631 0.17 -0.5 2,811,790 - Psest_2631 0.17 +1.4 2,811,962 + Psest_2631 0.36 -0.5 2,811,962 + Psest_2631 0.36 -0.9 2,811,962 + Psest_2631 0.36 +0.1 2,811,962 + Psest_2631 0.36 +3.1 2,811,962 + Psest_2631 0.36 +0.5 2,811,962 + Psest_2631 0.36 +0.2 2,811,963 - Psest_2631 0.37 -0.8 2,811,963 - Psest_2631 0.37 -0.2 2,811,963 - Psest_2631 0.37 +0.3 2,811,963 - Psest_2631 0.37 -3.2 2,811,963 - Psest_2631 0.37 +0.5 2,812,254 - Psest_2631 0.70 +0.0 2,812,265 + Psest_2631 0.71 +0.9 2,812,266 - Psest_2631 0.71 -0.4
Or see this region's nucleotide sequence