Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2300

Experiment: RCH2 grown on a colony of N2A2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2297 and Psest_2298 are separated by 150 nucleotidesPsest_2298 and Psest_2299 are separated by 51 nucleotidesPsest_2299 and Psest_2300 are separated by 32 nucleotidesPsest_2300 and Psest_2301 are separated by 273 nucleotides Psest_2297: Psest_2297 - hypothetical protein, at 2,448,974 to 2,450,401 _2297 Psest_2298: Psest_2298 - tRNA_Leu_TAG, at 2,450,552 to 2,450,636 _2298 Psest_2299: Psest_2299 - tRNA_His_GTG, at 2,450,688 to 2,450,763 _2299 Psest_2300: Psest_2300 - tRNA_Arg_TCT, at 2,450,796 to 2,450,872 _2300 Psest_2301: Psest_2301 - 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase, at 2,451,146 to 2,452,000 _2301 Position (kb) 2450 2451Strain fitness (log2 ratio) -1 0 1at 2450.018 kb on + strand, within Psest_2297at 2450.018 kb on + strand, within Psest_2297at 2450.184 kb on + strand, within Psest_2297at 2450.185 kb on - strand, within Psest_2297at 2450.452 kb on + strandat 2450.453 kb on - strandat 2450.456 kb on + strandat 2450.456 kb on + strandat 2450.457 kb on - strandat 2450.457 kb on - strandat 2450.529 kb on + strandat 2450.530 kb on - strandat 2450.530 kb on - strandat 2450.876 kb on - strandat 2451.037 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2
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2,450,018 + Psest_2297 0.73 +0.9
2,450,018 + Psest_2297 0.73 +1.1
2,450,184 + Psest_2297 0.85 -1.0
2,450,185 - Psest_2297 0.85 +0.8
2,450,452 + -0.8
2,450,453 - -1.6
2,450,456 + -1.3
2,450,456 + +0.3
2,450,457 - +0.3
2,450,457 - -0.2
2,450,529 + -0.1
2,450,530 - -0.3
2,450,530 - -1.3
2,450,876 - -0.3
2,451,037 + -1.6

Or see this region's nucleotide sequence