Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1601 and Psest_1602 overlap by 4 nucleotides Psest_1602 and Psest_1603 are separated by 139 nucleotides Psest_1603 and Psest_1604 overlap by 1 nucleotides Psest_1604 and Psest_1605 overlap by 4 nucleotides
Psest_1601: Psest_1601 - Signal transduction histidine kinase, at 1,704,503 to 1,705,825
_1601
Psest_1602: Psest_1602 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,705,822 to 1,706,499
_1602
Psest_1603: Psest_1603 - Thiol:disulfide interchange protein, at 1,706,639 to 1,708,375
_1603
Psest_1604: Psest_1604 - Thiol-disulfide isomerase and thioredoxins, at 1,708,375 to 1,709,190
_1604
Psest_1605: Psest_1605 - Protein-disulfide isomerase, at 1,709,187 to 1,709,954
_1605
Position (kb)
1706
1707
1708
1709 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1705.920 kb on + strand, within Psest_1602 at 1706.100 kb on - strand, within Psest_1602 at 1706.100 kb on - strand, within Psest_1602 at 1706.208 kb on - strand, within Psest_1602 at 1706.526 kb on + strand at 1706.544 kb on - strand at 1706.622 kb on + strand at 1706.623 kb on - strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.626 kb on + strand at 1706.627 kb on - strand at 1706.819 kb on + strand, within Psest_1603 at 1706.819 kb on + strand, within Psest_1603 at 1706.819 kb on + strand, within Psest_1603 at 1706.819 kb on + strand, within Psest_1603 at 1706.819 kb on + strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.820 kb on - strand, within Psest_1603 at 1706.826 kb on - strand, within Psest_1603 at 1706.928 kb on - strand, within Psest_1603 at 1707.269 kb on + strand, within Psest_1603 at 1707.269 kb on + strand, within Psest_1603 at 1707.269 kb on + strand, within Psest_1603 at 1707.269 kb on + strand, within Psest_1603 at 1707.270 kb on - strand, within Psest_1603 at 1707.270 kb on - strand, within Psest_1603 at 1707.270 kb on - strand, within Psest_1603 at 1707.971 kb on + strand, within Psest_1603 at 1707.972 kb on - strand, within Psest_1603 at 1708.094 kb on + strand, within Psest_1603 at 1708.097 kb on + strand, within Psest_1603 at 1708.097 kb on + strand, within Psest_1603 at 1708.098 kb on - strand, within Psest_1603 at 1708.120 kb on + strand, within Psest_1603 at 1708.121 kb on - strand, within Psest_1603 at 1708.249 kb on - strand at 1708.561 kb on + strand, within Psest_1604 at 1708.562 kb on - strand, within Psest_1604 at 1708.570 kb on + strand, within Psest_1604 at 1708.570 kb on + strand, within Psest_1604 at 1708.834 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.211 kb on + strand at 1709.358 kb on + strand, within Psest_1605
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 1,705,920 + Psest_1602 0.14 -0.7 1,706,100 - Psest_1602 0.41 +1.0 1,706,100 - Psest_1602 0.41 -0.1 1,706,208 - Psest_1602 0.57 -1.6 1,706,526 + +0.3 1,706,544 - +2.5 1,706,622 + -1.8 1,706,623 - -2.5 1,706,626 + -0.0 1,706,626 + +0.4 1,706,626 + +0.4 1,706,626 + -1.6 1,706,626 + -0.9 1,706,626 + -0.2 1,706,626 + -0.8 1,706,627 - -0.6 1,706,819 + Psest_1603 0.10 +0.2 1,706,819 + Psest_1603 0.10 -1.2 1,706,819 + Psest_1603 0.10 -1.2 1,706,819 + Psest_1603 0.10 +0.1 1,706,819 + Psest_1603 0.10 -0.3 1,706,820 - Psest_1603 0.10 +0.2 1,706,820 - Psest_1603 0.10 -1.6 1,706,820 - Psest_1603 0.10 -0.6 1,706,820 - Psest_1603 0.10 -0.2 1,706,820 - Psest_1603 0.10 -0.2 1,706,820 - Psest_1603 0.10 -1.4 1,706,826 - Psest_1603 0.11 +1.1 1,706,928 - Psest_1603 0.17 -1.5 1,707,269 + Psest_1603 0.36 -0.2 1,707,269 + Psest_1603 0.36 -1.2 1,707,269 + Psest_1603 0.36 -0.0 1,707,269 + Psest_1603 0.36 -2.3 1,707,270 - Psest_1603 0.36 +0.5 1,707,270 - Psest_1603 0.36 +0.0 1,707,270 - Psest_1603 0.36 +0.4 1,707,971 + Psest_1603 0.77 -0.7 1,707,972 - Psest_1603 0.77 -1.2 1,708,094 + Psest_1603 0.84 +0.6 1,708,097 + Psest_1603 0.84 +0.2 1,708,097 + Psest_1603 0.84 -0.5 1,708,098 - Psest_1603 0.84 +0.2 1,708,120 + Psest_1603 0.85 +0.7 1,708,121 - Psest_1603 0.85 -1.6 1,708,249 - -2.5 1,708,561 + Psest_1604 0.23 +1.3 1,708,562 - Psest_1604 0.23 +0.9 1,708,570 + Psest_1604 0.24 -0.6 1,708,570 + Psest_1604 0.24 -0.4 1,708,834 + Psest_1604 0.56 -0.2 1,709,089 + Psest_1604 0.88 -2.1 1,709,089 + Psest_1604 0.88 -0.4 1,709,089 + Psest_1604 0.88 -1.0 1,709,089 + Psest_1604 0.88 +2.4 1,709,211 + -1.3 1,709,358 + Psest_1605 0.22 +2.4
Or see this region's nucleotide sequence