Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0130 and Psest_0131 are separated by 11 nucleotides Psest_0131 and Psest_0132 are separated by 4 nucleotides Psest_0132 and Psest_0133 are separated by 26 nucleotides Psest_0133 and Psest_0134 are separated by 16 nucleotides
Psest_0130: Psest_0130 - Uncharacterized conserved protein, at 128,434 to 130,356
_0130
Psest_0131: Psest_0131 - type VI secretion system lysozyme-related protein, at 130,368 to 130,775
_0131
Psest_0132: Psest_0132 - type VI secretion protein, EvpB/VC_A0108 family, at 130,780 to 132,258
_0132
Psest_0133: Psest_0133 - type VI secretion protein, VC_A0107 family, at 132,285 to 132,791
_0133
Psest_0134: Psest_0134 - type VI secretion-associated protein, VC_A0119 family, at 132,808 to 134,370
_0134
Position (kb) 130
131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 129.944 kb on - strand, within Psest_0130 at 130.832 kb on + strand at 130.832 kb on + strand at 130.832 kb on + strand at 130.832 kb on + strand at 130.832 kb on + strand at 130.832 kb on + strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.833 kb on - strand at 130.936 kb on + strand, within Psest_0132 at 130.936 kb on + strand, within Psest_0132 at 130.936 kb on + strand, within Psest_0132 at 130.936 kb on + strand, within Psest_0132 at 130.936 kb on + strand, within Psest_0132 at 130.936 kb on + strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 130.937 kb on - strand, within Psest_0132 at 131.057 kb on + strand, within Psest_0132 at 131.057 kb on + strand, within Psest_0132 at 131.088 kb on - strand, within Psest_0132 at 131.111 kb on + strand, within Psest_0132 at 131.111 kb on + strand, within Psest_0132 at 131.111 kb on + strand, within Psest_0132 at 131.112 kb on - strand, within Psest_0132 at 131.112 kb on - strand, within Psest_0132 at 131.112 kb on - strand, within Psest_0132 at 131.112 kb on - strand, within Psest_0132 at 131.250 kb on - strand, within Psest_0132 at 131.423 kb on + strand, within Psest_0132 at 131.423 kb on + strand, within Psest_0132 at 131.423 kb on + strand, within Psest_0132 at 131.423 kb on + strand, within Psest_0132 at 131.424 kb on - strand, within Psest_0132 at 131.424 kb on - strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.444 kb on + strand, within Psest_0132 at 131.445 kb on - strand, within Psest_0132 at 131.445 kb on - strand, within Psest_0132 at 131.445 kb on - strand, within Psest_0132 at 131.445 kb on - strand, within Psest_0132 at 131.477 kb on + strand, within Psest_0132 at 131.477 kb on + strand, within Psest_0132 at 131.478 kb on - strand, within Psest_0132 at 131.556 kb on - strand, within Psest_0132 at 131.729 kb on + strand, within Psest_0132 at 131.729 kb on + strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.730 kb on - strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.771 kb on + strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.772 kb on - strand, within Psest_0132 at 131.817 kb on - strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.828 kb on + strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.829 kb on - strand, within Psest_0132 at 131.840 kb on + strand, within Psest_0132 at 131.840 kb on + strand, within Psest_0132 at 131.840 kb on + strand, within Psest_0132 at 131.840 kb on + strand, within Psest_0132 at 131.840 kb on + strand, within Psest_0132 at 131.841 kb on - strand, within Psest_0132 at 131.841 kb on - strand, within Psest_0132 at 132.152 kb on + strand at 132.152 kb on + strand at 132.152 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.260 kb on + strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.261 kb on - strand at 132.283 kb on + strand at 132.283 kb on + strand at 132.361 kb on - strand, within Psest_0133 at 132.361 kb on - strand, within Psest_0133 at 133.016 kb on + strand, within Psest_0134 at 133.016 kb on + strand, within Psest_0134 at 133.016 kb on + strand, within Psest_0134 at 133.016 kb on + strand, within Psest_0134 at 133.017 kb on - strand, within Psest_0134 at 133.017 kb on - strand, within Psest_0134
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 129,944 - Psest_0130 0.79 -0.0 130,832 + -1.6 130,832 + -3.2 130,832 + -1.5 130,832 + -0.8 130,832 + -1.4 130,832 + -0.7 130,833 - -1.7 130,833 - +1.3 130,833 - -0.1 130,833 - -0.1 130,833 - +0.2 130,833 - -3.2 130,833 - -1.3 130,833 - -0.7 130,833 - -1.4 130,936 + Psest_0132 0.11 -0.0 130,936 + Psest_0132 0.11 +0.2 130,936 + Psest_0132 0.11 +1.3 130,936 + Psest_0132 0.11 -0.6 130,936 + Psest_0132 0.11 +0.2 130,936 + Psest_0132 0.11 +0.4 130,937 - Psest_0132 0.11 -0.9 130,937 - Psest_0132 0.11 -0.6 130,937 - Psest_0132 0.11 +0.9 130,937 - Psest_0132 0.11 +0.2 130,937 - Psest_0132 0.11 -2.4 130,937 - Psest_0132 0.11 +0.1 131,057 + Psest_0132 0.19 -0.2 131,057 + Psest_0132 0.19 -2.1 131,088 - Psest_0132 0.21 +1.2 131,111 + Psest_0132 0.22 +0.2 131,111 + Psest_0132 0.22 -0.8 131,111 + Psest_0132 0.22 +0.2 131,112 - Psest_0132 0.22 +0.4 131,112 - Psest_0132 0.22 +0.2 131,112 - Psest_0132 0.22 -2.1 131,112 - Psest_0132 0.22 -2.5 131,250 - Psest_0132 0.32 +1.2 131,423 + Psest_0132 0.43 -1.1 131,423 + Psest_0132 0.43 -2.2 131,423 + Psest_0132 0.43 +0.4 131,423 + Psest_0132 0.43 +0.6 131,424 - Psest_0132 0.44 -1.8 131,424 - Psest_0132 0.44 -0.4 131,444 + Psest_0132 0.45 +0.5 131,444 + Psest_0132 0.45 +0.0 131,444 + Psest_0132 0.45 -0.1 131,444 + Psest_0132 0.45 -0.8 131,444 + Psest_0132 0.45 +1.6 131,444 + Psest_0132 0.45 -2.3 131,445 - Psest_0132 0.45 +1.5 131,445 - Psest_0132 0.45 +0.1 131,445 - Psest_0132 0.45 -1.9 131,445 - Psest_0132 0.45 -2.6 131,477 + Psest_0132 0.47 +1.9 131,477 + Psest_0132 0.47 +0.8 131,478 - Psest_0132 0.47 -2.5 131,556 - Psest_0132 0.52 -1.0 131,729 + Psest_0132 0.64 -0.9 131,729 + Psest_0132 0.64 +1.4 131,730 - Psest_0132 0.64 -0.4 131,730 - Psest_0132 0.64 +0.3 131,730 - Psest_0132 0.64 -1.9 131,730 - Psest_0132 0.64 +0.0 131,730 - Psest_0132 0.64 -0.1 131,730 - Psest_0132 0.64 -0.6 131,730 - Psest_0132 0.64 -0.9 131,771 + Psest_0132 0.67 -1.4 131,771 + Psest_0132 0.67 -1.3 131,771 + Psest_0132 0.67 +1.9 131,771 + Psest_0132 0.67 -2.8 131,771 + Psest_0132 0.67 -0.0 131,771 + Psest_0132 0.67 -2.2 131,771 + Psest_0132 0.67 +3.1 131,772 - Psest_0132 0.67 -0.6 131,772 - Psest_0132 0.67 +0.1 131,772 - Psest_0132 0.67 -1.4 131,772 - Psest_0132 0.67 -0.6 131,772 - Psest_0132 0.67 +1.2 131,772 - Psest_0132 0.67 -1.2 131,772 - Psest_0132 0.67 -1.1 131,772 - Psest_0132 0.67 -1.0 131,817 - Psest_0132 0.70 +1.2 131,828 + Psest_0132 0.71 -2.4 131,828 + Psest_0132 0.71 +1.0 131,828 + Psest_0132 0.71 -0.7 131,828 + Psest_0132 0.71 +0.5 131,828 + Psest_0132 0.71 -0.0 131,828 + Psest_0132 0.71 +0.1 131,828 + Psest_0132 0.71 +0.8 131,828 + Psest_0132 0.71 -0.5 131,828 + Psest_0132 0.71 -1.7 131,828 + Psest_0132 0.71 -1.4 131,828 + Psest_0132 0.71 -1.4 131,828 + Psest_0132 0.71 -0.4 131,828 + Psest_0132 0.71 -0.8 131,828 + Psest_0132 0.71 -0.5 131,829 - Psest_0132 0.71 +0.2 131,829 - Psest_0132 0.71 -2.0 131,829 - Psest_0132 0.71 -1.4 131,829 - Psest_0132 0.71 +0.6 131,829 - Psest_0132 0.71 -0.3 131,829 - Psest_0132 0.71 -0.3 131,829 - Psest_0132 0.71 -2.0 131,829 - Psest_0132 0.71 +0.9 131,829 - Psest_0132 0.71 +1.7 131,829 - Psest_0132 0.71 +0.5 131,829 - Psest_0132 0.71 -0.6 131,829 - Psest_0132 0.71 -0.3 131,840 + Psest_0132 0.72 +0.2 131,840 + Psest_0132 0.72 -1.1 131,840 + Psest_0132 0.72 +0.5 131,840 + Psest_0132 0.72 -1.7 131,840 + Psest_0132 0.72 -1.7 131,841 - Psest_0132 0.72 -0.5 131,841 - Psest_0132 0.72 -1.1 132,152 + +4.0 132,152 + -2.6 132,152 + -0.4 132,260 + -0.8 132,260 + -1.6 132,260 + -0.3 132,260 + -2.6 132,260 + -2.4 132,260 + -1.9 132,260 + +0.0 132,260 + -0.8 132,260 + +1.6 132,260 + +0.7 132,260 + -0.1 132,260 + -2.4 132,260 + -0.1 132,260 + -0.6 132,260 + -0.3 132,260 + -1.3 132,260 + -0.1 132,260 + +1.9 132,261 - -2.4 132,261 - -2.9 132,261 - -0.1 132,261 - -0.2 132,261 - -0.2 132,261 - -0.3 132,261 - +1.1 132,261 - -0.2 132,261 - +2.3 132,261 - -1.6 132,261 - -0.4 132,261 - +3.3 132,261 - -1.6 132,261 - -0.1 132,283 + +1.3 132,283 + -0.4 132,361 - Psest_0133 0.15 +0.7 132,361 - Psest_0133 0.15 +0.1 133,016 + Psest_0134 0.13 +0.4 133,016 + Psest_0134 0.13 +0.6 133,016 + Psest_0134 0.13 +0.1 133,016 + Psest_0134 0.13 -0.1 133,017 - Psest_0134 0.13 +5.8 133,017 - Psest_0134 0.13 -0.7
Or see this region's nucleotide sequence