Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0664 and Psest_0665 are separated by 84 nucleotides Psest_0665 and Psest_0666 are separated by 105 nucleotides Psest_0666 and Psest_0667 are separated by 147 nucleotides
Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652
_0664
Psest_0665: Psest_0665 - Inorganic pyrophosphatase, at 724,737 to 725,264
_0665
Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182
_0666
Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883
_0667
Position (kb) 725
726
727 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 724.490 kb on - strand, within Psest_0664 at 724.548 kb on + strand at 724.548 kb on + strand at 724.549 kb on - strand at 724.576 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.611 kb on + strand at 724.611 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.739 kb on - strand at 724.739 kb on - strand at 725.326 kb on - strand at 725.371 kb on + strand at 725.372 kb on - strand at 725.461 kb on + strand, within Psest_0666 at 725.462 kb on - strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.510 kb on - strand, within Psest_0666 at 725.614 kb on + strand, within Psest_0666 at 725.737 kb on + strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 725.738 kb on - strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.033 kb on + strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.034 kb on - strand, within Psest_0666 at 726.216 kb on + strand at 726.216 kb on + strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.217 kb on - strand at 726.250 kb on + strand at 726.251 kb on - strand at 726.293 kb on - strand at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.822 kb on + strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.823 kb on - strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.879 kb on + strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.880 kb on - strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.997 kb on - strand, within Psest_0667 at 727.002 kb on + strand, within Psest_0667
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 724,490 - Psest_0664 0.89 -1.4 724,548 + +1.5 724,548 + -0.9 724,549 - +0.4 724,576 + -2.0 724,578 + -1.9 724,578 + +4.7 724,578 + +1.0 724,578 + +0.9 724,578 + -1.2 724,579 - +0.8 724,579 - -0.1 724,579 - -0.9 724,579 - +1.4 724,579 - -1.7 724,611 + +0.6 724,611 + -1.4 724,738 + +0.4 724,738 + +0.3 724,738 + -0.4 724,739 - +0.1 724,739 - +0.8 725,326 - +1.6 725,371 + +1.8 725,372 - -0.0 725,461 + Psest_0666 0.11 -1.2 725,462 - Psest_0666 0.11 -3.3 725,475 + Psest_0666 0.13 -0.1 725,475 + Psest_0666 0.13 +1.9 725,476 - Psest_0666 0.13 +2.0 725,476 - Psest_0666 0.13 +0.3 725,509 + Psest_0666 0.17 +0.7 725,509 + Psest_0666 0.17 -0.8 725,510 - Psest_0666 0.17 -0.6 725,614 + Psest_0666 0.30 -0.2 725,737 + Psest_0666 0.45 +0.0 725,738 - Psest_0666 0.45 +2.1 725,738 - Psest_0666 0.45 +3.2 725,738 - Psest_0666 0.45 -0.2 725,738 - Psest_0666 0.45 +1.3 725,738 - Psest_0666 0.45 +0.7 725,738 - Psest_0666 0.45 +5.2 725,738 - Psest_0666 0.45 -1.2 726,033 + Psest_0666 0.82 -1.4 726,033 + Psest_0666 0.82 +0.7 726,033 + Psest_0666 0.82 +0.8 726,033 + Psest_0666 0.82 +0.8 726,033 + Psest_0666 0.82 -1.4 726,034 - Psest_0666 0.82 -0.4 726,034 - Psest_0666 0.82 +0.7 726,216 + +0.2 726,216 + -0.9 726,217 - -0.1 726,217 - +3.6 726,217 - -1.5 726,217 - +1.4 726,217 - -1.7 726,250 + +0.7 726,251 - -1.2 726,293 - +1.0 726,822 + Psest_0667 0.32 +1.9 726,822 + Psest_0667 0.32 +0.6 726,822 + Psest_0667 0.32 +1.3 726,823 - Psest_0667 0.32 -0.7 726,823 - Psest_0667 0.32 -1.3 726,823 - Psest_0667 0.32 +0.1 726,823 - Psest_0667 0.32 -0.4 726,879 + Psest_0667 0.35 +1.6 726,879 + Psest_0667 0.35 -1.3 726,879 + Psest_0667 0.35 +1.4 726,879 + Psest_0667 0.35 -0.6 726,879 + Psest_0667 0.35 +0.2 726,879 + Psest_0667 0.35 -1.4 726,879 + Psest_0667 0.35 -0.6 726,879 + Psest_0667 0.35 -1.0 726,880 - Psest_0667 0.35 +0.0 726,880 - Psest_0667 0.35 -3.5 726,880 - Psest_0667 0.35 +0.3 726,996 + Psest_0667 0.43 +0.4 726,996 + Psest_0667 0.43 -0.7 726,997 - Psest_0667 0.43 -1.2 727,002 + Psest_0667 0.43 +0.5
Or see this region's nucleotide sequence