Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4172 and Psest_4173 are separated by 20 nucleotides Psest_4173 and Psest_4174 are separated by 20 nucleotides Psest_4174 and Psest_4175 are separated by 407 nucleotides
Psest_4172: Psest_4172 - flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF), at 4,350,463 to 4,352,139
_4172
Psest_4173: Psest_4173 - flagellar hook-basal body complex protein FliE, at 4,352,160 to 4,352,480
_4173
Psest_4174: Psest_4174 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 4,352,501 to 4,353,913
_4174
Psest_4175: Psest_4175 - Flagellar motor switch/type III secretory pathway protein, at 4,354,321 to 4,355,160
_4175
Position (kb)
4352
4353
4354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4351.699 kb on + strand, within Psest_4172 at 4351.699 kb on + strand, within Psest_4172 at 4351.700 kb on - strand, within Psest_4172 at 4351.700 kb on - strand, within Psest_4172 at 4351.945 kb on + strand, within Psest_4172 at 4351.946 kb on - strand, within Psest_4172 at 4351.946 kb on - strand, within Psest_4172 at 4352.024 kb on + strand at 4352.024 kb on + strand at 4352.024 kb on + strand at 4352.024 kb on + strand at 4352.144 kb on + strand at 4352.144 kb on + strand at 4352.144 kb on + strand at 4352.144 kb on + strand at 4352.145 kb on - strand at 4352.145 kb on - strand at 4352.145 kb on - strand at 4352.898 kb on + strand, within Psest_4174 at 4352.898 kb on + strand, within Psest_4174 at 4352.899 kb on - strand, within Psest_4174 at 4352.899 kb on - strand, within Psest_4174 at 4353.346 kb on - strand, within Psest_4174 at 4353.546 kb on + strand, within Psest_4174 at 4353.546 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.620 kb on + strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.621 kb on - strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.660 kb on + strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.661 kb on - strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.662 kb on + strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.663 kb on - strand, within Psest_4174 at 4353.860 kb on + strand at 4353.860 kb on + strand at 4353.860 kb on + strand at 4353.861 kb on - strand at 4353.876 kb on + strand at 4353.876 kb on + strand at 4353.876 kb on + strand at 4353.876 kb on + strand at 4353.877 kb on - strand at 4353.877 kb on - strand at 4353.895 kb on + strand at 4354.080 kb on - strand at 4354.117 kb on + strand at 4354.199 kb on + strand at 4354.309 kb on - strand at 4354.374 kb on - strand at 4354.420 kb on + strand, within Psest_4175 at 4354.420 kb on + strand, within Psest_4175 at 4354.421 kb on - strand, within Psest_4175 at 4354.421 kb on - strand, within Psest_4175 at 4354.421 kb on - strand, within Psest_4175 at 4354.424 kb on + strand, within Psest_4175 at 4354.424 kb on + strand, within Psest_4175 at 4354.425 kb on - strand, within Psest_4175 at 4354.425 kb on - strand, within Psest_4175 at 4354.425 kb on - strand, within Psest_4175 at 4354.425 kb on - strand, within Psest_4175 at 4354.425 kb on - strand, within Psest_4175 at 4354.465 kb on + strand, within Psest_4175 at 4354.550 kb on - strand, within Psest_4175 at 4354.550 kb on - strand, within Psest_4175 at 4354.600 kb on - strand, within Psest_4175 at 4354.600 kb on - strand, within Psest_4175 at 4354.637 kb on + strand, within Psest_4175 at 4354.637 kb on + strand, within Psest_4175 at 4354.638 kb on - strand, within Psest_4175 at 4354.665 kb on + strand, within Psest_4175 at 4354.665 kb on + strand, within Psest_4175 at 4354.665 kb on + strand, within Psest_4175 at 4354.715 kb on + strand, within Psest_4175 at 4354.716 kb on - strand, within Psest_4175 at 4354.777 kb on + strand, within Psest_4175 at 4354.798 kb on + strand, within Psest_4175 at 4354.798 kb on + strand, within Psest_4175 at 4354.798 kb on + strand, within Psest_4175 at 4354.798 kb on + strand, within Psest_4175 at 4354.799 kb on - strand, within Psest_4175 at 4354.799 kb on - strand, within Psest_4175 at 4354.817 kb on - strand, within Psest_4175 at 4354.817 kb on - strand, within Psest_4175 at 4354.817 kb on - strand, within Psest_4175 at 4354.817 kb on - strand, within Psest_4175 at 4354.817 kb on - strand, within Psest_4175 at 4354.827 kb on + strand, within Psest_4175 at 4354.827 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.852 kb on + strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.853 kb on - strand, within Psest_4175 at 4354.858 kb on + strand, within Psest_4175 at 4354.858 kb on + strand, within Psest_4175 at 4354.858 kb on + strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175 at 4354.859 kb on - strand, within Psest_4175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 4,351,699 + Psest_4172 0.74 -1.5 4,351,699 + Psest_4172 0.74 -0.7 4,351,700 - Psest_4172 0.74 +3.3 4,351,700 - Psest_4172 0.74 +1.2 4,351,945 + Psest_4172 0.88 +0.3 4,351,946 - Psest_4172 0.88 -1.7 4,351,946 - Psest_4172 0.88 -1.4 4,352,024 + +1.5 4,352,024 + -1.7 4,352,024 + -1.6 4,352,024 + +0.2 4,352,144 + +3.2 4,352,144 + -0.0 4,352,144 + -0.1 4,352,144 + -1.6 4,352,145 - -1.4 4,352,145 - +0.6 4,352,145 - -0.4 4,352,898 + Psest_4174 0.28 -0.8 4,352,898 + Psest_4174 0.28 -0.8 4,352,899 - Psest_4174 0.28 +3.7 4,352,899 - Psest_4174 0.28 +0.3 4,353,346 - Psest_4174 0.60 +1.1 4,353,546 + Psest_4174 0.74 +0.6 4,353,546 + Psest_4174 0.74 +5.0 4,353,620 + Psest_4174 0.79 +2.4 4,353,620 + Psest_4174 0.79 -0.4 4,353,620 + Psest_4174 0.79 +0.7 4,353,620 + Psest_4174 0.79 +3.4 4,353,620 + Psest_4174 0.79 +5.7 4,353,620 + Psest_4174 0.79 -0.1 4,353,620 + Psest_4174 0.79 -0.3 4,353,620 + Psest_4174 0.79 -1.4 4,353,620 + Psest_4174 0.79 +0.3 4,353,620 + Psest_4174 0.79 +1.1 4,353,620 + Psest_4174 0.79 +1.0 4,353,620 + Psest_4174 0.79 +2.3 4,353,620 + Psest_4174 0.79 +1.2 4,353,620 + Psest_4174 0.79 +0.6 4,353,620 + Psest_4174 0.79 -0.4 4,353,621 - Psest_4174 0.79 -0.4 4,353,621 - Psest_4174 0.79 -1.6 4,353,621 - Psest_4174 0.79 -0.6 4,353,621 - Psest_4174 0.79 -1.3 4,353,621 - Psest_4174 0.79 -2.2 4,353,621 - Psest_4174 0.79 +0.0 4,353,621 - Psest_4174 0.79 -1.6 4,353,621 - Psest_4174 0.79 +0.9 4,353,621 - Psest_4174 0.79 -1.3 4,353,621 - Psest_4174 0.79 -2.0 4,353,621 - Psest_4174 0.79 -2.6 4,353,621 - Psest_4174 0.79 -3.1 4,353,621 - Psest_4174 0.79 +3.8 4,353,621 - Psest_4174 0.79 -0.7 4,353,621 - Psest_4174 0.79 +0.1 4,353,621 - Psest_4174 0.79 +4.3 4,353,621 - Psest_4174 0.79 -0.1 4,353,621 - Psest_4174 0.79 +1.3 4,353,621 - Psest_4174 0.79 -0.1 4,353,621 - Psest_4174 0.79 +0.7 4,353,621 - Psest_4174 0.79 -0.2 4,353,621 - Psest_4174 0.79 +0.2 4,353,621 - Psest_4174 0.79 +0.1 4,353,621 - Psest_4174 0.79 -3.0 4,353,621 - Psest_4174 0.79 +0.2 4,353,660 + Psest_4174 0.82 +0.0 4,353,660 + Psest_4174 0.82 +1.2 4,353,660 + Psest_4174 0.82 +0.8 4,353,660 + Psest_4174 0.82 +1.4 4,353,660 + Psest_4174 0.82 -0.5 4,353,660 + Psest_4174 0.82 +0.6 4,353,661 - Psest_4174 0.82 +0.8 4,353,661 - Psest_4174 0.82 +1.5 4,353,661 - Psest_4174 0.82 -0.1 4,353,661 - Psest_4174 0.82 -0.4 4,353,661 - Psest_4174 0.82 -1.4 4,353,661 - Psest_4174 0.82 -0.6 4,353,661 - Psest_4174 0.82 -0.4 4,353,661 - Psest_4174 0.82 -1.3 4,353,661 - Psest_4174 0.82 +0.4 4,353,661 - Psest_4174 0.82 -1.0 4,353,661 - Psest_4174 0.82 -0.7 4,353,661 - Psest_4174 0.82 +0.4 4,353,661 - Psest_4174 0.82 -0.5 4,353,661 - Psest_4174 0.82 -1.7 4,353,661 - Psest_4174 0.82 -1.4 4,353,661 - Psest_4174 0.82 -0.5 4,353,662 + Psest_4174 0.82 -1.8 4,353,662 + Psest_4174 0.82 +1.7 4,353,662 + Psest_4174 0.82 +0.3 4,353,662 + Psest_4174 0.82 +0.6 4,353,662 + Psest_4174 0.82 +3.0 4,353,662 + Psest_4174 0.82 +0.5 4,353,662 + Psest_4174 0.82 +0.7 4,353,662 + Psest_4174 0.82 -1.4 4,353,662 + Psest_4174 0.82 -1.1 4,353,662 + Psest_4174 0.82 +0.8 4,353,663 - Psest_4174 0.82 +0.5 4,353,663 - Psest_4174 0.82 -0.8 4,353,663 - Psest_4174 0.82 +0.2 4,353,663 - Psest_4174 0.82 -0.5 4,353,663 - Psest_4174 0.82 -1.3 4,353,663 - Psest_4174 0.82 +0.3 4,353,663 - Psest_4174 0.82 +0.4 4,353,663 - Psest_4174 0.82 -1.1 4,353,663 - Psest_4174 0.82 +0.1 4,353,663 - Psest_4174 0.82 +0.2 4,353,663 - Psest_4174 0.82 +1.0 4,353,663 - Psest_4174 0.82 -1.3 4,353,663 - Psest_4174 0.82 +1.7 4,353,663 - Psest_4174 0.82 +0.9 4,353,663 - Psest_4174 0.82 +1.6 4,353,663 - Psest_4174 0.82 -1.7 4,353,663 - Psest_4174 0.82 +0.2 4,353,663 - Psest_4174 0.82 -1.8 4,353,663 - Psest_4174 0.82 -1.7 4,353,663 - Psest_4174 0.82 -0.2 4,353,663 - Psest_4174 0.82 +0.3 4,353,860 + +1.1 4,353,860 + +1.6 4,353,860 + -2.6 4,353,861 - -0.7 4,353,876 + -2.0 4,353,876 + +0.3 4,353,876 + -0.5 4,353,876 + +0.7 4,353,877 - -0.3 4,353,877 - -0.7 4,353,895 + +4.7 4,354,080 - +1.6 4,354,117 + -0.2 4,354,199 + +0.7 4,354,309 - +1.2 4,354,374 - +0.2 4,354,420 + Psest_4175 0.12 -0.2 4,354,420 + Psest_4175 0.12 -0.7 4,354,421 - Psest_4175 0.12 +0.3 4,354,421 - Psest_4175 0.12 -0.9 4,354,421 - Psest_4175 0.12 +0.4 4,354,424 + Psest_4175 0.12 -0.6 4,354,424 + Psest_4175 0.12 +0.1 4,354,425 - Psest_4175 0.12 -1.8 4,354,425 - Psest_4175 0.12 +1.8 4,354,425 - Psest_4175 0.12 +0.0 4,354,425 - Psest_4175 0.12 +0.1 4,354,425 - Psest_4175 0.12 -1.0 4,354,465 + Psest_4175 0.17 -0.0 4,354,550 - Psest_4175 0.27 -0.2 4,354,550 - Psest_4175 0.27 -0.8 4,354,600 - Psest_4175 0.33 -0.1 4,354,600 - Psest_4175 0.33 +2.4 4,354,637 + Psest_4175 0.38 -1.4 4,354,637 + Psest_4175 0.38 +0.0 4,354,638 - Psest_4175 0.38 -0.6 4,354,665 + Psest_4175 0.41 -1.6 4,354,665 + Psest_4175 0.41 +0.9 4,354,665 + Psest_4175 0.41 +1.3 4,354,715 + Psest_4175 0.47 +0.4 4,354,716 - Psest_4175 0.47 +0.8 4,354,777 + Psest_4175 0.54 +0.1 4,354,798 + Psest_4175 0.57 +0.4 4,354,798 + Psest_4175 0.57 +1.8 4,354,798 + Psest_4175 0.57 -0.5 4,354,798 + Psest_4175 0.57 +1.4 4,354,799 - Psest_4175 0.57 -0.7 4,354,799 - Psest_4175 0.57 -0.8 4,354,817 - Psest_4175 0.59 +0.4 4,354,817 - Psest_4175 0.59 -2.8 4,354,817 - Psest_4175 0.59 -0.7 4,354,817 - Psest_4175 0.59 -0.4 4,354,817 - Psest_4175 0.59 +1.6 4,354,827 + Psest_4175 0.60 +0.2 4,354,827 + Psest_4175 0.60 +0.1 4,354,852 + Psest_4175 0.63 +0.6 4,354,852 + Psest_4175 0.63 +1.3 4,354,852 + Psest_4175 0.63 -0.2 4,354,852 + Psest_4175 0.63 +0.5 4,354,852 + Psest_4175 0.63 +0.8 4,354,852 + Psest_4175 0.63 -1.2 4,354,852 + Psest_4175 0.63 -1.8 4,354,852 + Psest_4175 0.63 -0.5 4,354,853 - Psest_4175 0.63 -2.2 4,354,853 - Psest_4175 0.63 +0.3 4,354,853 - Psest_4175 0.63 -1.1 4,354,853 - Psest_4175 0.63 +3.9 4,354,853 - Psest_4175 0.63 +0.4 4,354,853 - Psest_4175 0.63 +1.2 4,354,853 - Psest_4175 0.63 -0.8 4,354,853 - Psest_4175 0.63 -3.0 4,354,853 - Psest_4175 0.63 -0.5 4,354,853 - Psest_4175 0.63 -2.9 4,354,853 - Psest_4175 0.63 +0.9 4,354,853 - Psest_4175 0.63 -0.5 4,354,853 - Psest_4175 0.63 -0.9 4,354,853 - Psest_4175 0.63 -0.4 4,354,853 - Psest_4175 0.63 +1.6 4,354,858 + Psest_4175 0.64 +0.8 4,354,858 + Psest_4175 0.64 +0.5 4,354,858 + Psest_4175 0.64 -1.9 4,354,859 - Psest_4175 0.64 -1.1 4,354,859 - Psest_4175 0.64 -0.3 4,354,859 - Psest_4175 0.64 -1.5 4,354,859 - Psest_4175 0.64 +1.4 4,354,859 - Psest_4175 0.64 -0.2 4,354,859 - Psest_4175 0.64 +0.2
Or see this region's nucleotide sequence