Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2083 and Psest_2084 are separated by 58 nucleotides Psest_2084 and Psest_2085 are separated by 181 nucleotides Psest_2085 and Psest_2086 are separated by 13 nucleotides
Psest_2083: Psest_2083 - Putative sterol carrier protein, at 2,212,845 to 2,213,159
_2083
Psest_2084: Psest_2084 - Fructose-2,6-bisphosphatase, at 2,213,218 to 2,213,916
_2084
Psest_2085: Psest_2085 - Periplasmic serine proteases (ClpP class), at 2,214,098 to 2,215,120
_2085
Psest_2086: Psest_2086 - putative quinone oxidoreductase, YhdH/YhfP family, at 2,215,134 to 2,216,126
_2086
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.224 kb on - strand at 2213.224 kb on - strand at 2213.272 kb on - strand at 2213.272 kb on - strand at 2213.272 kb on - strand at 2213.390 kb on - strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.607 kb on + strand, within Psest_2084 at 2213.811 kb on - strand, within Psest_2084 at 2213.861 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.904 kb on - strand at 2213.953 kb on + strand at 2214.092 kb on + strand at 2214.092 kb on + strand at 2214.092 kb on + strand at 2214.093 kb on - strand at 2214.095 kb on + strand at 2214.095 kb on + strand at 2214.095 kb on + strand at 2214.096 kb on - strand at 2214.096 kb on - strand at 2214.096 kb on - strand at 2214.251 kb on + strand, within Psest_2085 at 2214.516 kb on - strand, within Psest_2085 at 2214.605 kb on + strand, within Psest_2085 at 2214.749 kb on + strand, within Psest_2085 at 2214.824 kb on - strand, within Psest_2085 at 2214.962 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.011 kb on + strand, within Psest_2085 at 2215.011 kb on + strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.060 kb on + strand at 2215.153 kb on + strand at 2215.251 kb on + strand, within Psest_2086 at 2215.280 kb on - strand, within Psest_2086 at 2215.314 kb on + strand, within Psest_2086 at 2215.446 kb on + strand, within Psest_2086 at 2215.516 kb on + strand, within Psest_2086 at 2215.517 kb on - strand, within Psest_2086 at 2215.628 kb on + strand, within Psest_2086 at 2215.659 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.675 kb on + strand, within Psest_2086 at 2215.916 kb on + strand, within Psest_2086 at 2215.916 kb on + strand, within Psest_2086 at 2216.113 kb on + strand at 2216.113 kb on + strand at 2216.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 2,213,223 + -1.4 2,213,223 + +0.6 2,213,223 + -2.8 2,213,223 + -1.2 2,213,223 + +0.5 2,213,224 - +1.8 2,213,224 - +3.6 2,213,272 - -1.2 2,213,272 - -2.3 2,213,272 - +0.7 2,213,390 - Psest_2084 0.25 +0.8 2,213,555 + Psest_2084 0.48 -0.1 2,213,555 + Psest_2084 0.48 -1.1 2,213,555 + Psest_2084 0.48 -0.6 2,213,555 + Psest_2084 0.48 -0.4 2,213,556 - Psest_2084 0.48 -0.5 2,213,556 - Psest_2084 0.48 -1.0 2,213,556 - Psest_2084 0.48 -0.1 2,213,556 - Psest_2084 0.48 -1.1 2,213,556 - Psest_2084 0.48 +1.3 2,213,556 - Psest_2084 0.48 +0.6 2,213,607 + Psest_2084 0.56 -1.4 2,213,811 - Psest_2084 0.85 +0.4 2,213,861 + -1.7 2,213,903 + -0.1 2,213,903 + +0.7 2,213,903 + +2.5 2,213,903 + -0.4 2,213,904 - -0.7 2,213,953 + +1.6 2,214,092 + -0.1 2,214,092 + -1.5 2,214,092 + -0.5 2,214,093 - -0.7 2,214,095 + -0.4 2,214,095 + -1.4 2,214,095 + +0.7 2,214,096 - -0.8 2,214,096 - -0.8 2,214,096 - +2.6 2,214,251 + Psest_2085 0.15 +1.5 2,214,516 - Psest_2085 0.41 +1.4 2,214,605 + Psest_2085 0.50 +1.3 2,214,749 + Psest_2085 0.64 +3.1 2,214,824 - Psest_2085 0.71 +0.4 2,214,962 + Psest_2085 0.84 -0.6 2,215,007 + Psest_2085 0.89 -2.3 2,215,007 + Psest_2085 0.89 -2.5 2,215,007 + Psest_2085 0.89 +0.6 2,215,007 + Psest_2085 0.89 +3.5 2,215,007 + Psest_2085 0.89 -1.0 2,215,007 + Psest_2085 0.89 -0.3 2,215,007 + Psest_2085 0.89 +1.7 2,215,008 - Psest_2085 0.89 +0.0 2,215,008 - Psest_2085 0.89 +4.5 2,215,008 - Psest_2085 0.89 +0.5 2,215,008 - Psest_2085 0.89 -2.4 2,215,008 - Psest_2085 0.89 -1.2 2,215,008 - Psest_2085 0.89 -0.9 2,215,011 + Psest_2085 0.89 +0.5 2,215,011 + Psest_2085 0.89 +0.1 2,215,012 - Psest_2085 0.89 +2.0 2,215,012 - Psest_2085 0.89 +0.8 2,215,012 - Psest_2085 0.89 +1.6 2,215,012 - Psest_2085 0.89 -0.1 2,215,060 + -0.6 2,215,153 + -1.9 2,215,251 + Psest_2086 0.12 -1.2 2,215,280 - Psest_2086 0.15 +0.8 2,215,314 + Psest_2086 0.18 +0.9 2,215,446 + Psest_2086 0.31 -0.9 2,215,516 + Psest_2086 0.38 +0.0 2,215,517 - Psest_2086 0.39 -0.4 2,215,628 + Psest_2086 0.50 +1.6 2,215,659 + Psest_2086 0.53 +1.7 2,215,661 + Psest_2086 0.53 -0.2 2,215,661 + Psest_2086 0.53 +1.0 2,215,661 + Psest_2086 0.53 +0.6 2,215,661 + Psest_2086 0.53 +3.9 2,215,661 + Psest_2086 0.53 -1.2 2,215,661 + Psest_2086 0.53 +0.0 2,215,662 - Psest_2086 0.53 +0.4 2,215,662 - Psest_2086 0.53 -1.4 2,215,662 - Psest_2086 0.53 -0.1 2,215,675 + Psest_2086 0.54 +2.9 2,215,916 + Psest_2086 0.79 -0.2 2,215,916 + Psest_2086 0.79 -0.3 2,216,113 + -1.6 2,216,113 + +0.0 2,216,114 - -1.6
Or see this region's nucleotide sequence