Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1227 and Psest_1228 are separated by 69 nucleotides Psest_1228 and Psest_1229 are separated by 109 nucleotides
Psest_1227: Psest_1227 - Predicted SAM-dependent methyltransferase, at 1,333,345 to 1,334,337
_1227
Psest_1228: Psest_1228 - Arabinose efflux permease, at 1,334,407 to 1,335,576
_1228
Psest_1229: Psest_1229 - Di- and tricarboxylate transporters, at 1,335,686 to 1,337,512
_1229
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1333.452 kb on + strand, within Psest_1227 at 1333.452 kb on + strand, within Psest_1227 at 1333.452 kb on + strand, within Psest_1227 at 1333.453 kb on - strand, within Psest_1227 at 1334.042 kb on + strand, within Psest_1227 at 1334.076 kb on - strand, within Psest_1227 at 1334.076 kb on - strand, within Psest_1227 at 1334.512 kb on - strand at 1334.549 kb on + strand, within Psest_1228 at 1334.729 kb on + strand, within Psest_1228 at 1334.729 kb on + strand, within Psest_1228 at 1334.729 kb on + strand, within Psest_1228 at 1334.730 kb on - strand, within Psest_1228 at 1334.730 kb on - strand, within Psest_1228 at 1334.730 kb on - strand, within Psest_1228 at 1334.826 kb on + strand, within Psest_1228 at 1334.826 kb on + strand, within Psest_1228 at 1334.827 kb on - strand, within Psest_1228 at 1334.900 kb on + strand, within Psest_1228 at 1334.900 kb on + strand, within Psest_1228 at 1334.900 kb on + strand, within Psest_1228 at 1334.901 kb on - strand, within Psest_1228 at 1334.901 kb on - strand, within Psest_1228 at 1334.901 kb on - strand, within Psest_1228 at 1334.901 kb on - strand, within Psest_1228 at 1335.098 kb on + strand, within Psest_1228 at 1335.099 kb on - strand, within Psest_1228 at 1335.158 kb on + strand, within Psest_1228 at 1335.158 kb on + strand, within Psest_1228 at 1335.159 kb on - strand, within Psest_1228 at 1335.413 kb on + strand, within Psest_1228 at 1335.491 kb on + strand at 1335.492 kb on - strand at 1335.492 kb on - strand at 1335.682 kb on + strand at 1335.683 kb on - strand at 1336.134 kb on + strand, within Psest_1229 at 1336.135 kb on - strand, within Psest_1229 at 1336.135 kb on - strand, within Psest_1229 at 1336.135 kb on - strand, within Psest_1229 at 1336.135 kb on - strand, within Psest_1229 at 1336.570 kb on - strand, within Psest_1229 at 1336.573 kb on - strand, within Psest_1229
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 1,333,452 + Psest_1227 0.11 -0.6 1,333,452 + Psest_1227 0.11 +0.1 1,333,452 + Psest_1227 0.11 +1.8 1,333,453 - Psest_1227 0.11 -0.5 1,334,042 + Psest_1227 0.70 -1.3 1,334,076 - Psest_1227 0.74 +1.7 1,334,076 - Psest_1227 0.74 -1.2 1,334,512 - +2.0 1,334,549 + Psest_1228 0.12 -1.0 1,334,729 + Psest_1228 0.28 -0.1 1,334,729 + Psest_1228 0.28 +0.7 1,334,729 + Psest_1228 0.28 -1.9 1,334,730 - Psest_1228 0.28 +0.9 1,334,730 - Psest_1228 0.28 +3.8 1,334,730 - Psest_1228 0.28 +0.6 1,334,826 + Psest_1228 0.36 -2.0 1,334,826 + Psest_1228 0.36 +1.0 1,334,827 - Psest_1228 0.36 +0.0 1,334,900 + Psest_1228 0.42 +2.7 1,334,900 + Psest_1228 0.42 +0.3 1,334,900 + Psest_1228 0.42 +1.4 1,334,901 - Psest_1228 0.42 -0.6 1,334,901 - Psest_1228 0.42 +0.1 1,334,901 - Psest_1228 0.42 -0.3 1,334,901 - Psest_1228 0.42 +0.7 1,335,098 + Psest_1228 0.59 -0.6 1,335,099 - Psest_1228 0.59 -0.7 1,335,158 + Psest_1228 0.64 -2.1 1,335,158 + Psest_1228 0.64 +0.9 1,335,159 - Psest_1228 0.64 +0.6 1,335,413 + Psest_1228 0.86 +2.8 1,335,491 + +0.5 1,335,492 - +0.5 1,335,492 - -1.3 1,335,682 + -2.0 1,335,683 - +1.5 1,336,134 + Psest_1229 0.25 -1.6 1,336,135 - Psest_1229 0.25 +0.4 1,336,135 - Psest_1229 0.25 -0.3 1,336,135 - Psest_1229 0.25 -0.2 1,336,135 - Psest_1229 0.25 -0.3 1,336,570 - Psest_1229 0.48 +2.1 1,336,573 - Psest_1229 0.49 -1.9
Or see this region's nucleotide sequence