Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1200 and Psest_1201 are separated by 280 nucleotides Psest_1201 and Psest_1202 are separated by 200 nucleotides Psest_1202 and Psest_1203 are separated by 80 nucleotides
Psest_1200: Psest_1200 - putative urate catabolism protein, at 1,303,240 to 1,304,172
_1200
Psest_1201: Psest_1201 - hydroxyisourate hydrolase, at 1,304,453 to 1,304,806
_1201
Psest_1202: Psest_1202 - Putative threonine efflux protein, at 1,305,007 to 1,305,633
_1202
Psest_1203: Psest_1203 - Permeases, at 1,305,714 to 1,307,060
_1203
Position (kb)
1305
1306 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1304.069 kb on + strand, within Psest_1200 at 1304.070 kb on - strand, within Psest_1200 at 1304.159 kb on + strand at 1304.188 kb on - strand at 1304.249 kb on + strand at 1304.262 kb on - strand at 1304.270 kb on + strand at 1304.271 kb on - strand at 1304.321 kb on + strand at 1304.476 kb on - strand at 1304.476 kb on - strand at 1304.526 kb on - strand, within Psest_1201 at 1304.618 kb on + strand, within Psest_1201 at 1304.633 kb on + strand, within Psest_1201 at 1304.634 kb on - strand, within Psest_1201 at 1304.660 kb on + strand, within Psest_1201 at 1304.661 kb on - strand, within Psest_1201 at 1304.668 kb on + strand, within Psest_1201 at 1304.668 kb on + strand, within Psest_1201 at 1304.750 kb on + strand, within Psest_1201 at 1304.751 kb on - strand, within Psest_1201 at 1304.751 kb on - strand, within Psest_1201 at 1304.751 kb on - strand, within Psest_1201 at 1304.783 kb on + strand at 1304.783 kb on + strand at 1304.784 kb on - strand at 1304.784 kb on - strand at 1304.831 kb on + strand at 1304.831 kb on + strand at 1304.869 kb on + strand at 1304.870 kb on - strand at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.073 kb on + strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.074 kb on - strand, within Psest_1202 at 1305.129 kb on - strand, within Psest_1202 at 1305.268 kb on + strand, within Psest_1202 at 1305.269 kb on - strand, within Psest_1202 at 1305.269 kb on - strand, within Psest_1202 at 1305.269 kb on - strand, within Psest_1202 at 1305.269 kb on - strand, within Psest_1202 at 1305.269 kb on - strand, within Psest_1202 at 1305.380 kb on + strand, within Psest_1202 at 1305.380 kb on + strand, within Psest_1202 at 1305.381 kb on - strand, within Psest_1202 at 1305.381 kb on - strand, within Psest_1202 at 1305.381 kb on - strand, within Psest_1202 at 1305.381 kb on - strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.389 kb on + strand, within Psest_1202 at 1305.390 kb on - strand, within Psest_1202 at 1305.527 kb on - strand, within Psest_1202 at 1305.533 kb on + strand, within Psest_1202 at 1305.533 kb on + strand, within Psest_1202 at 1305.534 kb on - strand, within Psest_1202 at 1305.650 kb on + strand at 1305.757 kb on + strand at 1305.757 kb on + strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.758 kb on - strand at 1305.811 kb on + strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.812 kb on - strand at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.952 kb on + strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.953 kb on - strand, within Psest_1203 at 1305.976 kb on + strand, within Psest_1203 at 1305.976 kb on + strand, within Psest_1203 at 1306.096 kb on + strand, within Psest_1203 at 1306.096 kb on + strand, within Psest_1203 at 1306.096 kb on + strand, within Psest_1203 at 1306.096 kb on + strand, within Psest_1203 at 1306.097 kb on - strand, within Psest_1203 at 1306.097 kb on - strand, within Psest_1203 at 1306.295 kb on + strand, within Psest_1203 at 1306.366 kb on + strand, within Psest_1203 at 1306.367 kb on - strand, within Psest_1203 at 1306.434 kb on + strand, within Psest_1203 at 1306.434 kb on + strand, within Psest_1203 at 1306.435 kb on - strand, within Psest_1203 at 1306.435 kb on - strand, within Psest_1203 at 1306.435 kb on - strand, within Psest_1203
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 1,304,069 + Psest_1200 0.89 -2.3 1,304,070 - Psest_1200 0.89 -0.6 1,304,159 + -0.6 1,304,188 - -0.4 1,304,249 + -2.3 1,304,262 - +0.7 1,304,270 + -2.0 1,304,271 - +0.5 1,304,321 + -2.3 1,304,476 - -0.4 1,304,476 - -0.9 1,304,526 - Psest_1201 0.21 -1.9 1,304,618 + Psest_1201 0.47 -1.5 1,304,633 + Psest_1201 0.51 -2.2 1,304,634 - Psest_1201 0.51 +0.8 1,304,660 + Psest_1201 0.58 -1.9 1,304,661 - Psest_1201 0.59 +3.1 1,304,668 + Psest_1201 0.61 -2.7 1,304,668 + Psest_1201 0.61 -0.0 1,304,750 + Psest_1201 0.84 +0.2 1,304,751 - Psest_1201 0.84 -1.7 1,304,751 - Psest_1201 0.84 +0.3 1,304,751 - Psest_1201 0.84 -2.4 1,304,783 + -1.4 1,304,783 + -0.0 1,304,784 - +0.1 1,304,784 - +0.5 1,304,831 + -1.0 1,304,831 + -0.3 1,304,869 + -1.9 1,304,870 - -3.0 1,305,073 + Psest_1202 0.11 +0.6 1,305,073 + Psest_1202 0.11 +0.1 1,305,073 + Psest_1202 0.11 +0.1 1,305,073 + Psest_1202 0.11 -4.0 1,305,073 + Psest_1202 0.11 -0.1 1,305,073 + Psest_1202 0.11 +1.7 1,305,073 + Psest_1202 0.11 +0.2 1,305,073 + Psest_1202 0.11 -0.3 1,305,073 + Psest_1202 0.11 +2.1 1,305,073 + Psest_1202 0.11 -0.4 1,305,073 + Psest_1202 0.11 -2.2 1,305,073 + Psest_1202 0.11 +0.7 1,305,073 + Psest_1202 0.11 -1.1 1,305,073 + Psest_1202 0.11 +0.1 1,305,073 + Psest_1202 0.11 -0.2 1,305,073 + Psest_1202 0.11 +0.4 1,305,073 + Psest_1202 0.11 -0.8 1,305,073 + Psest_1202 0.11 -3.5 1,305,073 + Psest_1202 0.11 +0.6 1,305,073 + Psest_1202 0.11 -0.7 1,305,073 + Psest_1202 0.11 -0.8 1,305,074 - Psest_1202 0.11 +0.4 1,305,074 - Psest_1202 0.11 -1.7 1,305,074 - Psest_1202 0.11 +0.7 1,305,074 - Psest_1202 0.11 -0.9 1,305,074 - Psest_1202 0.11 -1.6 1,305,074 - Psest_1202 0.11 -0.0 1,305,074 - Psest_1202 0.11 -0.3 1,305,074 - Psest_1202 0.11 +0.2 1,305,074 - Psest_1202 0.11 +0.0 1,305,074 - Psest_1202 0.11 -0.3 1,305,074 - Psest_1202 0.11 +1.6 1,305,074 - Psest_1202 0.11 -0.3 1,305,074 - Psest_1202 0.11 +1.5 1,305,074 - Psest_1202 0.11 +5.0 1,305,074 - Psest_1202 0.11 -0.6 1,305,074 - Psest_1202 0.11 -1.1 1,305,074 - Psest_1202 0.11 -2.1 1,305,074 - Psest_1202 0.11 -2.5 1,305,074 - Psest_1202 0.11 +0.8 1,305,074 - Psest_1202 0.11 +0.1 1,305,074 - Psest_1202 0.11 +0.2 1,305,074 - Psest_1202 0.11 +0.4 1,305,074 - Psest_1202 0.11 +0.5 1,305,074 - Psest_1202 0.11 -2.5 1,305,074 - Psest_1202 0.11 +2.0 1,305,074 - Psest_1202 0.11 +1.7 1,305,074 - Psest_1202 0.11 -1.1 1,305,074 - Psest_1202 0.11 -0.6 1,305,129 - Psest_1202 0.19 +0.9 1,305,268 + Psest_1202 0.42 -1.1 1,305,269 - Psest_1202 0.42 -1.0 1,305,269 - Psest_1202 0.42 -1.0 1,305,269 - Psest_1202 0.42 +2.6 1,305,269 - Psest_1202 0.42 -1.8 1,305,269 - Psest_1202 0.42 -2.4 1,305,380 + Psest_1202 0.59 -1.2 1,305,380 + Psest_1202 0.59 -1.0 1,305,381 - Psest_1202 0.60 +3.1 1,305,381 - Psest_1202 0.60 -2.0 1,305,381 - Psest_1202 0.60 -1.3 1,305,381 - Psest_1202 0.60 +0.5 1,305,389 + Psest_1202 0.61 -1.0 1,305,389 + Psest_1202 0.61 +0.6 1,305,389 + Psest_1202 0.61 -1.8 1,305,389 + Psest_1202 0.61 +0.6 1,305,389 + Psest_1202 0.61 -1.1 1,305,389 + Psest_1202 0.61 -3.1 1,305,389 + Psest_1202 0.61 -0.8 1,305,389 + Psest_1202 0.61 -0.9 1,305,390 - Psest_1202 0.61 -0.8 1,305,527 - Psest_1202 0.83 -0.9 1,305,533 + Psest_1202 0.84 -2.3 1,305,533 + Psest_1202 0.84 +1.2 1,305,534 - Psest_1202 0.84 -1.9 1,305,650 + +0.1 1,305,757 + +0.0 1,305,757 + -1.7 1,305,758 - -0.9 1,305,758 - -0.2 1,305,758 - -0.3 1,305,758 - +0.1 1,305,758 - +1.1 1,305,758 - -0.6 1,305,758 - +0.2 1,305,758 - -0.9 1,305,758 - -0.6 1,305,758 - -0.7 1,305,758 - -2.3 1,305,758 - +1.4 1,305,811 + -0.9 1,305,812 - +3.1 1,305,812 - -0.8 1,305,812 - -0.6 1,305,812 - -0.7 1,305,812 - -0.2 1,305,812 - -1.3 1,305,812 - +5.2 1,305,952 + Psest_1203 0.18 +1.6 1,305,952 + Psest_1203 0.18 -1.4 1,305,952 + Psest_1203 0.18 +1.4 1,305,952 + Psest_1203 0.18 -0.9 1,305,952 + Psest_1203 0.18 +0.0 1,305,952 + Psest_1203 0.18 +0.1 1,305,952 + Psest_1203 0.18 +5.2 1,305,952 + Psest_1203 0.18 +2.7 1,305,952 + Psest_1203 0.18 -0.9 1,305,952 + Psest_1203 0.18 -1.7 1,305,952 + Psest_1203 0.18 -1.4 1,305,952 + Psest_1203 0.18 -1.5 1,305,952 + Psest_1203 0.18 +1.4 1,305,952 + Psest_1203 0.18 -0.3 1,305,953 - Psest_1203 0.18 -0.0 1,305,953 - Psest_1203 0.18 +1.5 1,305,953 - Psest_1203 0.18 +0.1 1,305,953 - Psest_1203 0.18 -1.1 1,305,953 - Psest_1203 0.18 -0.4 1,305,953 - Psest_1203 0.18 +0.6 1,305,953 - Psest_1203 0.18 +0.1 1,305,953 - Psest_1203 0.18 +0.5 1,305,953 - Psest_1203 0.18 +0.0 1,305,953 - Psest_1203 0.18 +1.5 1,305,953 - Psest_1203 0.18 -1.1 1,305,953 - Psest_1203 0.18 +0.1 1,305,976 + Psest_1203 0.19 -1.5 1,305,976 + Psest_1203 0.19 -0.7 1,306,096 + Psest_1203 0.28 -0.9 1,306,096 + Psest_1203 0.28 -0.1 1,306,096 + Psest_1203 0.28 +0.2 1,306,096 + Psest_1203 0.28 +0.5 1,306,097 - Psest_1203 0.28 +1.9 1,306,097 - Psest_1203 0.28 -0.8 1,306,295 + Psest_1203 0.43 +2.5 1,306,366 + Psest_1203 0.48 +0.8 1,306,367 - Psest_1203 0.48 +2.5 1,306,434 + Psest_1203 0.53 -0.8 1,306,434 + Psest_1203 0.53 +0.7 1,306,435 - Psest_1203 0.54 +2.9 1,306,435 - Psest_1203 0.54 -0.8 1,306,435 - Psest_1203 0.54 -0.6
Or see this region's nucleotide sequence