Experiment: RCH2 grown on a colony of N2A2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0111 and Psest_0112 are separated by 332 nucleotides Psest_0112 and Psest_0113 are separated by 58 nucleotides Psest_0113 and Psest_0114 overlap by 4 nucleotides
Psest_0111: Psest_0111 - Glycine/D-amino acid oxidases (deaminating), at 109,205 to 110,464
_0111
Psest_0112: Psest_0112 - Transcriptional regulator, at 110,797 to 111,714
_0112
Psest_0113: Psest_0113 - Uncharacterized protein conserved in bacteria, at 111,773 to 112,414
_0113
Psest_0114: Psest_0114 - RNA polymerase sigma factor, sigma-70 family, at 112,411 to 112,980
_0114
Position (kb)
110
111
112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 109.850 kb on + strand, within Psest_0111 at 109.851 kb on - strand, within Psest_0111 at 109.851 kb on - strand, within Psest_0111 at 110.027 kb on + strand, within Psest_0111 at 110.172 kb on - strand, within Psest_0111 at 110.172 kb on - strand, within Psest_0111 at 110.172 kb on - strand, within Psest_0111 at 110.336 kb on + strand, within Psest_0111 at 110.336 kb on + strand, within Psest_0111 at 110.336 kb on + strand, within Psest_0111 at 110.336 kb on + strand, within Psest_0111 at 110.336 kb on + strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.337 kb on - strand, within Psest_0111 at 110.625 kb on + strand at 110.625 kb on + strand at 110.662 kb on + strand at 110.662 kb on + strand at 110.662 kb on + strand at 110.662 kb on + strand at 110.662 kb on + strand at 110.663 kb on - strand at 110.663 kb on - strand at 110.688 kb on - strand at 110.819 kb on + strand at 110.819 kb on + strand at 110.819 kb on + strand at 110.819 kb on + strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.949 kb on - strand, within Psest_0112 at 111.296 kb on + strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.401 kb on + strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.744 kb on + strand at 111.745 kb on - strand at 111.806 kb on - strand at 111.841 kb on - strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.577 kb on - strand, within Psest_0114
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2A2 remove 109,850 + Psest_0111 0.51 -2.2 109,851 - Psest_0111 0.51 -0.8 109,851 - Psest_0111 0.51 +3.0 110,027 + Psest_0111 0.65 -0.1 110,172 - Psest_0111 0.77 -1.2 110,172 - Psest_0111 0.77 +1.4 110,172 - Psest_0111 0.77 -0.0 110,336 + Psest_0111 0.90 -2.8 110,336 + Psest_0111 0.90 +0.4 110,336 + Psest_0111 0.90 +0.2 110,336 + Psest_0111 0.90 -0.6 110,336 + Psest_0111 0.90 -0.2 110,337 - Psest_0111 0.90 -0.7 110,337 - Psest_0111 0.90 +0.8 110,337 - Psest_0111 0.90 -1.2 110,337 - Psest_0111 0.90 -0.2 110,337 - Psest_0111 0.90 -1.7 110,337 - Psest_0111 0.90 -0.8 110,625 + -1.3 110,625 + +0.5 110,662 + +0.7 110,662 + -0.0 110,662 + +1.2 110,662 + +0.6 110,662 + -0.4 110,663 - +1.2 110,663 - -0.8 110,688 - -1.2 110,819 + +0.9 110,819 + +1.2 110,819 + +0.2 110,819 + +2.2 110,820 - -0.5 110,820 - +0.3 110,820 - -1.3 110,820 - -1.6 110,820 - -1.2 110,820 - -1.8 110,820 - +2.0 110,949 - Psest_0112 0.17 +0.1 111,296 + Psest_0112 0.54 -0.3 111,297 - Psest_0112 0.54 -1.8 111,297 - Psest_0112 0.54 -1.8 111,297 - Psest_0112 0.54 +1.5 111,297 - Psest_0112 0.54 +0.4 111,401 + Psest_0112 0.66 +0.0 111,402 - Psest_0112 0.66 -0.7 111,402 - Psest_0112 0.66 +0.0 111,402 - Psest_0112 0.66 -0.8 111,512 + Psest_0112 0.78 -0.5 111,512 + Psest_0112 0.78 -0.2 111,512 + Psest_0112 0.78 +0.2 111,512 + Psest_0112 0.78 -0.3 111,512 + Psest_0112 0.78 -0.2 111,512 + Psest_0112 0.78 -2.0 111,744 + -0.5 111,745 - -0.3 111,806 - -2.9 111,841 - Psest_0113 0.11 -3.4 112,281 + Psest_0113 0.79 -2.2 112,281 + Psest_0113 0.79 +0.5 112,577 - Psest_0114 0.29 -1.4
Or see this region's nucleotide sequence