Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3516 and Psest_3517 are separated by 145 nucleotides Psest_3517 and Psest_3518 are separated by 241 nucleotides Psest_3518 and Psest_3519 are separated by 236 nucleotides
Psest_3516: Psest_3516 - Leucine dehydrogenase (EC 1.4.1.9) (from data) , at 3,692,956 to 3,694,050
_3516
Psest_3517: Psest_3517 - branched-chain ketoacid ferredoxin reductase (EC 1.2.7.7) active on 4-methyl-2-oxopentanoate, (S)-3-methyl-2-oxopentanoate, or 3-methyl-2-oxobutanoate (from data) , at 3,694,196 to 3,697,666
_3517
Psest_3518: Psest_3518 - Transcriptional regulators, at 3,697,908 to 3,698,378
_3518
Psest_3519: Psest_3519 - Uncharacterized conserved protein, at 3,698,615 to 3,699,031
_3519
Position (kb)
3694
3695
3696
3697
3698 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3693.356 kb on + strand, within Psest_3516 at 3693.356 kb on + strand, within Psest_3516 at 3693.356 kb on + strand, within Psest_3516 at 3693.357 kb on - strand, within Psest_3516 at 3693.720 kb on + strand, within Psest_3516 at 3693.720 kb on + strand, within Psest_3516 at 3693.721 kb on - strand, within Psest_3516 at 3693.721 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.723 kb on - strand, within Psest_3516 at 3693.908 kb on + strand, within Psest_3516 at 3694.099 kb on + strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.100 kb on - strand at 3694.362 kb on + strand at 3694.611 kb on + strand, within Psest_3517 at 3694.612 kb on - strand, within Psest_3517 at 3694.612 kb on - strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.662 kb on + strand, within Psest_3517 at 3694.680 kb on + strand, within Psest_3517 at 3694.692 kb on + strand, within Psest_3517 at 3694.692 kb on + strand, within Psest_3517 at 3694.693 kb on - strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.710 kb on + strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.711 kb on - strand, within Psest_3517 at 3694.714 kb on - strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.343 kb on + strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.344 kb on - strand, within Psest_3517 at 3695.582 kb on + strand, within Psest_3517 at 3695.859 kb on + strand, within Psest_3517 at 3695.860 kb on - strand, within Psest_3517 at 3695.860 kb on - strand, within Psest_3517 at 3696.096 kb on + strand, within Psest_3517 at 3696.097 kb on - strand, within Psest_3517 at 3696.108 kb on + strand, within Psest_3517 at 3696.108 kb on + strand, within Psest_3517 at 3696.108 kb on + strand, within Psest_3517 at 3696.109 kb on - strand, within Psest_3517 at 3696.109 kb on - strand, within Psest_3517 at 3696.168 kb on + strand, within Psest_3517 at 3696.168 kb on + strand, within Psest_3517 at 3696.168 kb on + strand, within Psest_3517 at 3696.168 kb on + strand, within Psest_3517 at 3696.168 kb on + strand, within Psest_3517 at 3696.169 kb on - strand, within Psest_3517 at 3696.169 kb on - strand, within Psest_3517 at 3696.169 kb on - strand, within Psest_3517 at 3696.169 kb on - strand, within Psest_3517 at 3696.169 kb on - strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.297 kb on + strand, within Psest_3517 at 3696.298 kb on - strand, within Psest_3517 at 3696.298 kb on - strand, within Psest_3517 at 3696.298 kb on - strand, within Psest_3517 at 3696.298 kb on - strand, within Psest_3517 at 3696.298 kb on - strand, within Psest_3517 at 3696.376 kb on - strand, within Psest_3517 at 3696.399 kb on + strand, within Psest_3517 at 3696.399 kb on + strand, within Psest_3517 at 3696.399 kb on + strand, within Psest_3517 at 3696.399 kb on + strand, within Psest_3517 at 3696.400 kb on - strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.471 kb on + strand, within Psest_3517 at 3696.472 kb on - strand, within Psest_3517 at 3696.606 kb on + strand, within Psest_3517 at 3696.606 kb on + strand, within Psest_3517 at 3696.607 kb on - strand, within Psest_3517 at 3696.807 kb on + strand, within Psest_3517 at 3696.808 kb on - strand, within Psest_3517 at 3696.808 kb on - strand, within Psest_3517 at 3696.808 kb on - strand, within Psest_3517 at 3696.903 kb on + strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.904 kb on - strand, within Psest_3517 at 3696.913 kb on - strand, within Psest_3517 at 3697.122 kb on + strand, within Psest_3517 at 3697.122 kb on + strand, within Psest_3517 at 3697.353 kb on + strand at 3697.353 kb on + strand at 3697.354 kb on - strand at 3697.354 kb on - strand at 3697.354 kb on - strand at 3697.458 kb on + strand at 3697.458 kb on + strand at 3697.458 kb on + strand at 3697.608 kb on + strand at 3697.608 kb on + strand at 3697.608 kb on + strand at 3697.608 kb on + strand at 3697.609 kb on - strand at 3697.609 kb on - strand at 3697.609 kb on - strand at 3697.609 kb on - strand at 3697.632 kb on + strand at 3697.678 kb on - strand at 3697.678 kb on - strand at 3697.678 kb on - strand at 3697.852 kb on - strand at 3697.925 kb on - strand at 3698.268 kb on + strand, within Psest_3518 at 3698.269 kb on - strand, within Psest_3518 at 3698.376 kb on + strand at 3698.377 kb on - strand at 3698.434 kb on - strand at 3698.477 kb on - strand at 3698.616 kb on + strand at 3698.616 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 3,693,356 + Psest_3516 0.37 +1.8 3,693,356 + Psest_3516 0.37 -2.3 3,693,356 + Psest_3516 0.37 -1.2 3,693,357 - Psest_3516 0.37 -3.5 3,693,720 + Psest_3516 0.70 -4.2 3,693,720 + Psest_3516 0.70 +0.7 3,693,721 - Psest_3516 0.70 -1.1 3,693,721 - Psest_3516 0.70 -2.2 3,693,723 - Psest_3516 0.70 -0.6 3,693,723 - Psest_3516 0.70 +0.1 3,693,723 - Psest_3516 0.70 -2.5 3,693,908 + Psest_3516 0.87 +0.1 3,694,099 + -0.7 3,694,100 - +1.9 3,694,100 - +1.5 3,694,100 - -1.8 3,694,100 - -1.5 3,694,362 + +0.3 3,694,611 + Psest_3517 0.12 -0.5 3,694,612 - Psest_3517 0.12 +0.1 3,694,612 - Psest_3517 0.12 -1.3 3,694,662 + Psest_3517 0.13 -2.7 3,694,662 + Psest_3517 0.13 -1.0 3,694,662 + Psest_3517 0.13 -0.6 3,694,680 + Psest_3517 0.14 -0.5 3,694,692 + Psest_3517 0.14 -2.7 3,694,692 + Psest_3517 0.14 -2.6 3,694,693 - Psest_3517 0.14 -2.7 3,694,710 + Psest_3517 0.15 -1.7 3,694,710 + Psest_3517 0.15 -2.5 3,694,710 + Psest_3517 0.15 -0.5 3,694,710 + Psest_3517 0.15 -2.9 3,694,710 + Psest_3517 0.15 -2.0 3,694,710 + Psest_3517 0.15 -0.5 3,694,711 - Psest_3517 0.15 -0.5 3,694,711 - Psest_3517 0.15 +0.1 3,694,711 - Psest_3517 0.15 +0.3 3,694,711 - Psest_3517 0.15 -1.0 3,694,711 - Psest_3517 0.15 -2.3 3,694,711 - Psest_3517 0.15 -3.3 3,694,711 - Psest_3517 0.15 +1.5 3,694,714 - Psest_3517 0.15 -0.3 3,695,343 + Psest_3517 0.33 +0.3 3,695,343 + Psest_3517 0.33 -1.0 3,695,343 + Psest_3517 0.33 -0.8 3,695,343 + Psest_3517 0.33 -0.9 3,695,343 + Psest_3517 0.33 -0.5 3,695,343 + Psest_3517 0.33 -1.2 3,695,343 + Psest_3517 0.33 -0.5 3,695,343 + Psest_3517 0.33 +2.2 3,695,343 + Psest_3517 0.33 +0.1 3,695,343 + Psest_3517 0.33 +0.8 3,695,343 + Psest_3517 0.33 -0.5 3,695,343 + Psest_3517 0.33 -0.8 3,695,343 + Psest_3517 0.33 -1.2 3,695,343 + Psest_3517 0.33 +1.7 3,695,343 + Psest_3517 0.33 +0.4 3,695,343 + Psest_3517 0.33 -1.3 3,695,343 + Psest_3517 0.33 -2.2 3,695,343 + Psest_3517 0.33 -2.2 3,695,344 - Psest_3517 0.33 -2.2 3,695,344 - Psest_3517 0.33 +0.1 3,695,344 - Psest_3517 0.33 -0.8 3,695,344 - Psest_3517 0.33 +0.2 3,695,344 - Psest_3517 0.33 -1.1 3,695,344 - Psest_3517 0.33 -0.6 3,695,344 - Psest_3517 0.33 -2.3 3,695,344 - Psest_3517 0.33 +1.0 3,695,344 - Psest_3517 0.33 +0.9 3,695,344 - Psest_3517 0.33 -1.9 3,695,582 + Psest_3517 0.40 +0.8 3,695,859 + Psest_3517 0.48 -1.9 3,695,860 - Psest_3517 0.48 -2.7 3,695,860 - Psest_3517 0.48 -0.2 3,696,096 + Psest_3517 0.55 -0.0 3,696,097 - Psest_3517 0.55 +0.6 3,696,108 + Psest_3517 0.55 -2.7 3,696,108 + Psest_3517 0.55 +1.4 3,696,108 + Psest_3517 0.55 -0.5 3,696,109 - Psest_3517 0.55 +1.0 3,696,109 - Psest_3517 0.55 -1.4 3,696,168 + Psest_3517 0.57 -0.5 3,696,168 + Psest_3517 0.57 -0.4 3,696,168 + Psest_3517 0.57 +1.2 3,696,168 + Psest_3517 0.57 +1.2 3,696,168 + Psest_3517 0.57 +1.3 3,696,169 - Psest_3517 0.57 +1.1 3,696,169 - Psest_3517 0.57 -0.8 3,696,169 - Psest_3517 0.57 -2.8 3,696,169 - Psest_3517 0.57 -0.6 3,696,169 - Psest_3517 0.57 +0.0 3,696,297 + Psest_3517 0.61 +0.3 3,696,297 + Psest_3517 0.61 -2.3 3,696,297 + Psest_3517 0.61 -1.2 3,696,297 + Psest_3517 0.61 -0.7 3,696,297 + Psest_3517 0.61 +1.1 3,696,297 + Psest_3517 0.61 -0.7 3,696,297 + Psest_3517 0.61 +1.0 3,696,298 - Psest_3517 0.61 -1.7 3,696,298 - Psest_3517 0.61 +1.4 3,696,298 - Psest_3517 0.61 +1.3 3,696,298 - Psest_3517 0.61 -0.9 3,696,298 - Psest_3517 0.61 -0.8 3,696,376 - Psest_3517 0.63 -1.6 3,696,399 + Psest_3517 0.63 +1.3 3,696,399 + Psest_3517 0.63 +0.6 3,696,399 + Psest_3517 0.63 +0.0 3,696,399 + Psest_3517 0.63 +0.7 3,696,400 - Psest_3517 0.63 -1.2 3,696,471 + Psest_3517 0.66 +1.1 3,696,471 + Psest_3517 0.66 -1.4 3,696,471 + Psest_3517 0.66 -2.1 3,696,471 + Psest_3517 0.66 -1.1 3,696,471 + Psest_3517 0.66 -1.0 3,696,471 + Psest_3517 0.66 +2.8 3,696,472 - Psest_3517 0.66 -0.2 3,696,606 + Psest_3517 0.69 -1.7 3,696,606 + Psest_3517 0.69 +3.5 3,696,607 - Psest_3517 0.69 +0.2 3,696,807 + Psest_3517 0.75 -0.0 3,696,808 - Psest_3517 0.75 -4.2 3,696,808 - Psest_3517 0.75 -1.2 3,696,808 - Psest_3517 0.75 -1.6 3,696,903 + Psest_3517 0.78 -0.7 3,696,904 - Psest_3517 0.78 +2.4 3,696,904 - Psest_3517 0.78 -2.2 3,696,904 - Psest_3517 0.78 +1.2 3,696,904 - Psest_3517 0.78 -2.2 3,696,904 - Psest_3517 0.78 -0.2 3,696,904 - Psest_3517 0.78 +2.5 3,696,913 - Psest_3517 0.78 -1.5 3,697,122 + Psest_3517 0.84 +1.2 3,697,122 + Psest_3517 0.84 -0.3 3,697,353 + -1.0 3,697,353 + -1.9 3,697,354 - -1.9 3,697,354 - -1.0 3,697,354 - -2.0 3,697,458 + -0.8 3,697,458 + -1.3 3,697,458 + +0.6 3,697,608 + -0.0 3,697,608 + -2.1 3,697,608 + -1.2 3,697,608 + -1.8 3,697,609 - +0.3 3,697,609 - -1.0 3,697,609 - -3.9 3,697,609 - +2.6 3,697,632 + -1.0 3,697,678 - +1.7 3,697,678 - +0.2 3,697,678 - +0.8 3,697,852 - -0.4 3,697,925 - -1.9 3,698,268 + Psest_3518 0.76 -1.9 3,698,269 - Psest_3518 0.77 -1.5 3,698,376 + -2.7 3,698,377 - -2.2 3,698,434 - -0.0 3,698,477 - +0.9 3,698,616 + +1.2 3,698,616 + +0.3
Or see this region's nucleotide sequence