Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1006 and Psest_1007 are separated by 115 nucleotides Psest_1007 and Psest_1008 are separated by 41 nucleotides Psest_1008 and Psest_1009 are separated by 74 nucleotides
Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998
_1006
Psest_1007: Psest_1007 - Predicted permeases, at 1,092,114 to 1,093,013
_1007
Psest_1008: Psest_1008 - hypothetical protein, at 1,093,055 to 1,093,216
_1008
Psest_1009: Psest_1009 - hypothetical protein, at 1,093,291 to 1,094,049
_1009
Position (kb)
1092
1093
1094 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1091.255 kb on + strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.297 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.369 kb on - strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.412 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.529 kb on - strand, within Psest_1006 at 1091.705 kb on + strand, within Psest_1006 at 1091.706 kb on - strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.712 kb on - strand, within Psest_1006 at 1091.739 kb on - strand, within Psest_1006 at 1091.791 kb on - strand, within Psest_1006 at 1092.053 kb on + strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.166 kb on - strand at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.475 kb on - strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1093.023 kb on - strand at 1093.023 kb on - strand at 1093.023 kb on - strand at 1093.198 kb on - strand, within Psest_1008
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,091,255 + Psest_1006 0.19 +1.6 1,091,256 - Psest_1006 0.19 -0.4 1,091,256 - Psest_1006 0.19 +0.0 1,091,297 + Psest_1006 0.24 -3.5 1,091,368 + Psest_1006 0.31 -2.1 1,091,368 + Psest_1006 0.31 -1.4 1,091,368 + Psest_1006 0.31 +0.2 1,091,369 - Psest_1006 0.32 -2.9 1,091,381 + Psest_1006 0.33 +1.6 1,091,381 + Psest_1006 0.33 -1.0 1,091,381 + Psest_1006 0.33 +0.2 1,091,382 - Psest_1006 0.33 -1.8 1,091,382 - Psest_1006 0.33 -1.5 1,091,411 + Psest_1006 0.36 +1.4 1,091,411 + Psest_1006 0.36 +2.0 1,091,412 - Psest_1006 0.36 -1.9 1,091,448 - Psest_1006 0.40 +2.5 1,091,448 - Psest_1006 0.40 -2.8 1,091,448 - Psest_1006 0.40 +0.0 1,091,448 - Psest_1006 0.40 -2.0 1,091,529 - Psest_1006 0.49 +0.0 1,091,705 + Psest_1006 0.68 +0.4 1,091,706 - Psest_1006 0.68 -0.3 1,091,711 + Psest_1006 0.69 +1.2 1,091,711 + Psest_1006 0.69 -0.6 1,091,712 - Psest_1006 0.69 +0.5 1,091,739 - Psest_1006 0.72 +1.9 1,091,791 - Psest_1006 0.77 -1.7 1,092,053 + +2.0 1,092,054 - +0.4 1,092,054 - +1.4 1,092,054 - +0.2 1,092,166 - +0.9 1,092,316 - Psest_1007 0.22 +2.2 1,092,316 - Psest_1007 0.22 -0.6 1,092,316 - Psest_1007 0.22 -0.9 1,092,416 + Psest_1007 0.34 +1.2 1,092,416 + Psest_1007 0.34 -0.1 1,092,416 + Psest_1007 0.34 +4.9 1,092,474 + Psest_1007 0.40 +4.4 1,092,474 + Psest_1007 0.40 -0.6 1,092,474 + Psest_1007 0.40 -1.8 1,092,474 + Psest_1007 0.40 +0.2 1,092,474 + Psest_1007 0.40 -3.1 1,092,475 - Psest_1007 0.40 -2.5 1,092,531 + Psest_1007 0.46 +2.2 1,092,531 + Psest_1007 0.46 -0.2 1,092,532 - Psest_1007 0.46 +2.9 1,092,532 - Psest_1007 0.46 -1.9 1,092,532 - Psest_1007 0.46 -1.2 1,092,532 - Psest_1007 0.46 +0.3 1,092,532 - Psest_1007 0.46 -2.9 1,092,532 - Psest_1007 0.46 +1.5 1,092,532 - Psest_1007 0.46 +0.4 1,092,532 - Psest_1007 0.46 -1.1 1,092,559 + Psest_1007 0.49 +1.5 1,092,559 + Psest_1007 0.49 -0.1 1,092,559 + Psest_1007 0.49 -1.1 1,092,560 - Psest_1007 0.50 -1.8 1,092,560 - Psest_1007 0.50 -2.3 1,093,023 - -0.1 1,093,023 - -0.4 1,093,023 - +0.2 1,093,198 - Psest_1008 0.88 -2.5
Or see this region's nucleotide sequence