Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0086 and Psest_0087 are separated by 71 nucleotides Psest_0087 and Psest_0088 are separated by 273 nucleotides Psest_0088 and Psest_0089 overlap by 10 nucleotides Psest_0089 and Psest_0090 are separated by 98 nucleotides
Psest_0086: Psest_0086 - hypothetical protein, at 88,546 to 88,971
_0086
Psest_0087: Psest_0087 - hypothetical protein, at 89,043 to 89,285
_0087
Psest_0088: Psest_0088 - phosphonate metabolism protein PhnP, at 89,559 to 90,308
_0088
Psest_0089: Psest_0089 - phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN, at 90,299 to 90,853
_0089
Psest_0090: Psest_0090 - phosphonate metabolism protein PhnM, at 90,952 to 92,097
_0090
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 88.673 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 88.769 kb on - strand, within Psest_0086 at 89.112 kb on + strand, within Psest_0087 at 89.112 kb on + strand, within Psest_0087 at 89.112 kb on + strand, within Psest_0087 at 89.113 kb on - strand, within Psest_0087 at 89.113 kb on - strand, within Psest_0087 at 89.429 kb on - strand at 89.460 kb on - strand at 89.560 kb on + strand at 89.561 kb on - strand at 89.561 kb on - strand at 89.561 kb on - strand at 89.801 kb on + strand, within Psest_0088 at 89.802 kb on - strand, within Psest_0088 at 89.834 kb on - strand, within Psest_0088 at 89.867 kb on - strand, within Psest_0088 at 89.983 kb on + strand, within Psest_0088 at 89.983 kb on + strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 89.984 kb on - strand, within Psest_0088 at 90.259 kb on + strand at 90.259 kb on + strand at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.384 kb on + strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.385 kb on - strand, within Psest_0089 at 90.549 kb on + strand, within Psest_0089 at 90.621 kb on + strand, within Psest_0089 at 90.835 kb on - strand at 90.835 kb on - strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.898 kb on + strand at 90.899 kb on - strand at 90.899 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.010 kb on - strand at 91.182 kb on - strand, within Psest_0090
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 88,673 - Psest_0086 0.30 -3.1 88,769 - Psest_0086 0.52 -0.9 88,769 - Psest_0086 0.52 +0.7 88,769 - Psest_0086 0.52 -0.9 89,112 + Psest_0087 0.28 +0.8 89,112 + Psest_0087 0.28 +1.1 89,112 + Psest_0087 0.28 +0.8 89,113 - Psest_0087 0.29 +0.6 89,113 - Psest_0087 0.29 +1.8 89,429 - -1.7 89,460 - -2.2 89,560 + +1.5 89,561 - -1.6 89,561 - +1.5 89,561 - +1.7 89,801 + Psest_0088 0.32 -2.6 89,802 - Psest_0088 0.32 +0.8 89,834 - Psest_0088 0.37 -1.7 89,867 - Psest_0088 0.41 +1.4 89,983 + Psest_0088 0.57 -1.7 89,983 + Psest_0088 0.57 +0.0 89,984 - Psest_0088 0.57 -1.2 89,984 - Psest_0088 0.57 +1.0 89,984 - Psest_0088 0.57 -0.4 89,984 - Psest_0088 0.57 +1.6 90,259 + -2.2 90,259 + -0.9 90,384 + Psest_0089 0.15 -0.7 90,384 + Psest_0089 0.15 +3.5 90,384 + Psest_0089 0.15 -1.5 90,384 + Psest_0089 0.15 +2.1 90,384 + Psest_0089 0.15 -0.1 90,384 + Psest_0089 0.15 -1.7 90,385 - Psest_0089 0.15 -0.1 90,385 - Psest_0089 0.15 -0.0 90,385 - Psest_0089 0.15 +0.6 90,385 - Psest_0089 0.15 +0.0 90,385 - Psest_0089 0.15 -2.2 90,385 - Psest_0089 0.15 -2.3 90,385 - Psest_0089 0.15 +0.3 90,385 - Psest_0089 0.15 -0.7 90,549 + Psest_0089 0.45 +0.4 90,621 + Psest_0089 0.58 -2.0 90,835 - +1.1 90,835 - +3.6 90,898 + -1.5 90,898 + -0.6 90,898 + +2.4 90,898 + -2.6 90,898 + -3.6 90,898 + -0.5 90,898 + -0.7 90,898 + -1.2 90,899 - -1.2 90,899 - -1.2 91,010 - +0.3 91,010 - -2.5 91,010 - +1.8 91,010 - -0.5 91,010 - +2.1 91,182 - Psest_0090 0.20 +1.7
Or see this region's nucleotide sequence