Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0667 and Psest_0668 are separated by 6 nucleotides Psest_0668 and Psest_0669 are separated by 85 nucleotides
Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883
_0667
Psest_0668: Psest_0668 - Ethanolamine ammonia-lyase, small subunit, at 727,890 to 728,684
_0668
Psest_0669: Psest_0669 - Ethanolamine ammonia-lyase, large subunit, at 728,770 to 730,161
_0669
Position (kb) 727
728
729 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 726.996 kb on + strand, within Psest_0667 at 726.996 kb on + strand, within Psest_0667 at 726.997 kb on - strand, within Psest_0667 at 727.002 kb on + strand, within Psest_0667 at 727.224 kb on + strand, within Psest_0667 at 727.225 kb on - strand, within Psest_0667 at 727.239 kb on + strand, within Psest_0667 at 727.239 kb on + strand, within Psest_0667 at 727.302 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.334 kb on - strand, within Psest_0667 at 727.579 kb on - strand, within Psest_0667 at 727.593 kb on + strand, within Psest_0667 at 727.594 kb on - strand, within Psest_0667 at 727.594 kb on - strand, within Psest_0667 at 727.596 kb on + strand, within Psest_0667 at 727.596 kb on + strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.597 kb on - strand, within Psest_0667 at 727.617 kb on + strand, within Psest_0667 at 727.617 kb on + strand, within Psest_0667 at 727.817 kb on - strand at 727.817 kb on - strand at 727.849 kb on - strand at 727.938 kb on + strand at 727.962 kb on + strand at 728.002 kb on + strand, within Psest_0668 at 728.002 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.029 kb on + strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.030 kb on - strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.110 kb on + strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.111 kb on - strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.434 kb on + strand, within Psest_0668 at 728.435 kb on - strand at 728.483 kb on + strand, within Psest_0668 at 728.483 kb on + strand, within Psest_0668 at 728.483 kb on + strand, within Psest_0668 at 728.484 kb on - strand, within Psest_0668 at 728.484 kb on - strand, within Psest_0668 at 728.688 kb on + strand at 728.918 kb on + strand, within Psest_0669 at 728.918 kb on + strand, within Psest_0669 at 728.918 kb on + strand, within Psest_0669 at 728.918 kb on + strand, within Psest_0669 at 728.918 kb on + strand, within Psest_0669 at 728.918 kb on + strand, within Psest_0669 at 728.919 kb on - strand, within Psest_0669 at 728.919 kb on - strand, within Psest_0669 at 728.919 kb on - strand, within Psest_0669 at 728.919 kb on - strand, within Psest_0669 at 728.919 kb on - strand, within Psest_0669 at 728.948 kb on + strand, within Psest_0669 at 729.066 kb on - strand, within Psest_0669 at 729.066 kb on - strand, within Psest_0669 at 729.152 kb on + strand, within Psest_0669 at 729.152 kb on + strand, within Psest_0669 at 729.153 kb on - strand, within Psest_0669 at 729.158 kb on + strand, within Psest_0669 at 729.158 kb on + strand, within Psest_0669 at 729.158 kb on + strand, within Psest_0669 at 729.158 kb on + strand, within Psest_0669 at 729.159 kb on - strand, within Psest_0669 at 729.206 kb on + strand, within Psest_0669 at 729.206 kb on + strand, within Psest_0669 at 729.206 kb on + strand, within Psest_0669 at 729.206 kb on + strand, within Psest_0669 at 729.207 kb on - strand, within Psest_0669 at 729.207 kb on - strand, within Psest_0669 at 729.207 kb on - strand, within Psest_0669 at 729.224 kb on + strand, within Psest_0669 at 729.305 kb on + strand, within Psest_0669 at 729.305 kb on + strand, within Psest_0669 at 729.306 kb on - strand, within Psest_0669 at 729.357 kb on - strand, within Psest_0669 at 729.357 kb on - strand, within Psest_0669
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 726,996 + Psest_0667 0.43 -0.6 726,996 + Psest_0667 0.43 -2.8 726,997 - Psest_0667 0.43 +1.4 727,002 + Psest_0667 0.43 -1.3 727,224 + Psest_0667 0.58 -2.4 727,225 - Psest_0667 0.58 +2.9 727,239 + Psest_0667 0.58 +1.6 727,239 + Psest_0667 0.58 +0.3 727,302 - Psest_0667 0.63 +0.5 727,334 - Psest_0667 0.65 -0.1 727,334 - Psest_0667 0.65 -0.3 727,334 - Psest_0667 0.65 +0.7 727,334 - Psest_0667 0.65 +4.7 727,334 - Psest_0667 0.65 -2.1 727,579 - Psest_0667 0.80 -1.3 727,593 + Psest_0667 0.81 -0.3 727,594 - Psest_0667 0.81 +1.0 727,594 - Psest_0667 0.81 -0.9 727,596 + Psest_0667 0.81 -0.9 727,596 + Psest_0667 0.81 -0.5 727,597 - Psest_0667 0.82 +2.1 727,597 - Psest_0667 0.82 +1.4 727,597 - Psest_0667 0.82 -2.3 727,617 + Psest_0667 0.83 -2.0 727,617 + Psest_0667 0.83 -0.9 727,817 - +2.8 727,817 - +4.7 727,849 - -1.3 727,938 + -2.7 727,962 + -0.6 728,002 + Psest_0668 0.14 +0.6 728,002 + Psest_0668 0.14 +2.3 728,029 + Psest_0668 0.17 -1.3 728,029 + Psest_0668 0.17 +1.1 728,029 + Psest_0668 0.17 +1.0 728,029 + Psest_0668 0.17 -0.7 728,029 + Psest_0668 0.17 -2.3 728,029 + Psest_0668 0.17 -2.9 728,029 + Psest_0668 0.17 +2.3 728,030 - Psest_0668 0.18 -0.7 728,030 - Psest_0668 0.18 -1.7 728,030 - Psest_0668 0.18 +0.7 728,110 + Psest_0668 0.28 -2.4 728,110 + Psest_0668 0.28 -1.2 728,110 + Psest_0668 0.28 +1.6 728,110 + Psest_0668 0.28 +0.9 728,111 - Psest_0668 0.28 +1.2 728,111 - Psest_0668 0.28 -1.4 728,111 - Psest_0668 0.28 +0.3 728,111 - Psest_0668 0.28 -1.1 728,111 - Psest_0668 0.28 +0.3 728,434 + Psest_0668 0.68 +1.6 728,434 + Psest_0668 0.68 +0.2 728,434 + Psest_0668 0.68 -1.5 728,434 + Psest_0668 0.68 -1.0 728,435 - -1.0 728,483 + Psest_0668 0.75 +2.5 728,483 + Psest_0668 0.75 -1.1 728,483 + Psest_0668 0.75 -1.0 728,484 - Psest_0668 0.75 -1.5 728,484 - Psest_0668 0.75 -1.4 728,688 + +0.4 728,918 + Psest_0669 0.11 -0.5 728,918 + Psest_0669 0.11 -1.3 728,918 + Psest_0669 0.11 +0.7 728,918 + Psest_0669 0.11 -2.0 728,918 + Psest_0669 0.11 -0.3 728,918 + Psest_0669 0.11 -2.0 728,919 - Psest_0669 0.11 +2.2 728,919 - Psest_0669 0.11 -0.3 728,919 - Psest_0669 0.11 -1.0 728,919 - Psest_0669 0.11 +3.3 728,919 - Psest_0669 0.11 +1.4 728,948 + Psest_0669 0.13 -0.7 729,066 - Psest_0669 0.21 -1.3 729,066 - Psest_0669 0.21 +2.0 729,152 + Psest_0669 0.27 -1.9 729,152 + Psest_0669 0.27 -0.5 729,153 - Psest_0669 0.28 -1.6 729,158 + Psest_0669 0.28 +1.3 729,158 + Psest_0669 0.28 +1.9 729,158 + Psest_0669 0.28 +2.2 729,158 + Psest_0669 0.28 -2.6 729,159 - Psest_0669 0.28 -1.5 729,206 + Psest_0669 0.31 -1.7 729,206 + Psest_0669 0.31 -2.5 729,206 + Psest_0669 0.31 +1.6 729,206 + Psest_0669 0.31 +3.4 729,207 - Psest_0669 0.31 -2.4 729,207 - Psest_0669 0.31 -0.5 729,207 - Psest_0669 0.31 -2.1 729,224 + Psest_0669 0.33 -1.0 729,305 + Psest_0669 0.38 -0.3 729,305 + Psest_0669 0.38 -2.7 729,306 - Psest_0669 0.39 +3.2 729,357 - Psest_0669 0.42 -2.6 729,357 - Psest_0669 0.42 -1.6
Or see this region's nucleotide sequence