Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_4350

Experiment: RCH2 grown on a colony of Serratia

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_4349 and Psest_4350 are separated by 2 nucleotidesPsest_4350 and Psest_4352 are separated by 293 nucleotides Psest_4349: Psest_4349 - glucosamine--fructose-6-phosphate aminotransferase (isomerizing), at 4,549,153 to 4,551,003 _4349 Psest_4350: Psest_4350 - Transcriptional regulators of sugar metabolism, at 4,551,006 to 4,551,788 _4350 Psest_4352: Psest_4352 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 4,552,082 to 4,553,440 _4352 Position (kb) 4551 4552Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4551.226 kb on - strand, within Psest_4350at 4551.226 kb on - strand, within Psest_4350at 4551.226 kb on - strand, within Psest_4350at 4551.243 kb on - strand, within Psest_4350at 4551.560 kb on - strand, within Psest_4350at 4551.560 kb on - strand, within Psest_4350at 4551.560 kb on - strand, within Psest_4350at 4551.576 kb on + strand, within Psest_4350at 4551.576 kb on + strand, within Psest_4350at 4551.576 kb on + strand, within Psest_4350at 4551.576 kb on + strand, within Psest_4350at 4551.576 kb on + strand, within Psest_4350at 4551.577 kb on - strand, within Psest_4350at 4551.577 kb on - strand, within Psest_4350at 4551.577 kb on - strand, within Psest_4350at 4551.793 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia
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4,551,226 - Psest_4350 0.28 +0.9
4,551,226 - Psest_4350 0.28 -1.9
4,551,226 - Psest_4350 0.28 -2.2
4,551,243 - Psest_4350 0.30 -1.5
4,551,560 - Psest_4350 0.71 +3.3
4,551,560 - Psest_4350 0.71 +0.4
4,551,560 - Psest_4350 0.71 -1.4
4,551,576 + Psest_4350 0.73 -1.6
4,551,576 + Psest_4350 0.73 -3.6
4,551,576 + Psest_4350 0.73 +1.1
4,551,576 + Psest_4350 0.73 -2.2
4,551,576 + Psest_4350 0.73 -0.7
4,551,577 - Psest_4350 0.73 -2.8
4,551,577 - Psest_4350 0.73 -0.2
4,551,577 - Psest_4350 0.73 -1.1
4,551,793 - -1.9

Or see this region's nucleotide sequence