Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0378 and Psest_0379 are separated by 126 nucleotides Psest_0379 and Psest_0380 are separated by 159 nucleotides
Psest_0378: Psest_0378 - Lactate dehydrogenase and related dehydrogenases, at 406,715 to 407,644
_0378
Psest_0379: Psest_0379 - Galactose mutarotase and related enzymes, at 407,771 to 408,661
_0379
Psest_0380: Psest_0380 - TonB-dependent siderophore receptor, at 408,821 to 410,944
_0380
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.154 kb on + strand, within Psest_0378 at 407.154 kb on + strand, within Psest_0378 at 407.155 kb on - strand, within Psest_0378 at 407.155 kb on - strand, within Psest_0378 at 407.219 kb on + strand, within Psest_0378 at 407.220 kb on - strand, within Psest_0378 at 407.522 kb on - strand, within Psest_0378 at 407.677 kb on + strand at 407.908 kb on - strand, within Psest_0379 at 408.199 kb on - strand, within Psest_0379 at 408.254 kb on + strand, within Psest_0379 at 408.254 kb on + strand, within Psest_0379 at 408.254 kb on + strand, within Psest_0379 at 408.254 kb on + strand, within Psest_0379 at 408.254 kb on + strand, within Psest_0379 at 408.255 kb on - strand, within Psest_0379 at 408.255 kb on - strand, within Psest_0379 at 408.376 kb on - strand, within Psest_0379 at 408.506 kb on + strand, within Psest_0379 at 408.507 kb on - strand, within Psest_0379 at 408.507 kb on - strand, within Psest_0379 at 408.800 kb on + strand at 408.801 kb on - strand at 408.970 kb on + strand at 408.989 kb on + strand at 408.990 kb on - strand at 409.148 kb on + strand, within Psest_0380 at 409.149 kb on - strand, within Psest_0380 at 409.238 kb on + strand, within Psest_0380 at 409.238 kb on + strand, within Psest_0380 at 409.238 kb on + strand, within Psest_0380 at 409.239 kb on - strand, within Psest_0380 at 409.239 kb on - strand, within Psest_0380 at 409.239 kb on - strand, within Psest_0380 at 409.311 kb on - strand, within Psest_0380
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 407,154 + Psest_0378 0.47 +1.2 407,154 + Psest_0378 0.47 -1.9 407,155 - Psest_0378 0.47 -2.6 407,155 - Psest_0378 0.47 -0.3 407,219 + Psest_0378 0.54 -1.7 407,220 - Psest_0378 0.54 -0.2 407,522 - Psest_0378 0.87 -0.5 407,677 + +2.7 407,908 - Psest_0379 0.15 -0.1 408,199 - Psest_0379 0.48 +1.0 408,254 + Psest_0379 0.54 -2.0 408,254 + Psest_0379 0.54 +1.7 408,254 + Psest_0379 0.54 +1.2 408,254 + Psest_0379 0.54 +2.0 408,254 + Psest_0379 0.54 -2.6 408,255 - Psest_0379 0.54 +0.4 408,255 - Psest_0379 0.54 +2.3 408,376 - Psest_0379 0.68 +1.2 408,506 + Psest_0379 0.82 +1.6 408,507 - Psest_0379 0.83 +1.1 408,507 - Psest_0379 0.83 +1.8 408,800 + +0.0 408,801 - +0.8 408,970 + +3.0 408,989 + -0.7 408,990 - +3.5 409,148 + Psest_0380 0.15 -2.5 409,149 - Psest_0380 0.15 -3.3 409,238 + Psest_0380 0.20 +3.7 409,238 + Psest_0380 0.20 +2.0 409,238 + Psest_0380 0.20 -1.6 409,239 - Psest_0380 0.20 +3.2 409,239 - Psest_0380 0.20 +1.9 409,239 - Psest_0380 0.20 +2.3 409,311 - Psest_0380 0.23 +0.8
Or see this region's nucleotide sequence