Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3049 and Psest_3050 are separated by 80 nucleotides Psest_3050 and Psest_3051 are separated by 95 nucleotides Psest_3051 and Psest_3052 are separated by 62 nucleotides Psest_3052 and Psest_3053 overlap by 19 nucleotides
Psest_3049: Psest_3049 - pseudouridine synthase, at 3,231,616 to 3,232,308
_3049
Psest_3050: Psest_3050 - tRNA_Gly_CCC, at 3,232,389 to 3,232,462
_3050
Psest_3051: Psest_3051 - Predicted Fe-S-cluster oxidoreductase, at 3,232,558 to 3,233,229
_3051
Psest_3052: Psest_3052 - Site-specific recombinase XerD, at 3,233,292 to 3,234,299
_3052
Psest_3053: Psest_3053 - hypothetical protein, at 3,234,281 to 3,235,558
_3053
Position (kb)
3233
3234
3235 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3232.399 kb on + strand, within Psest_3050 at 3232.399 kb on + strand, within Psest_3050 at 3232.399 kb on + strand, within Psest_3050 at 3232.400 kb on - strand, within Psest_3050 at 3232.400 kb on - strand, within Psest_3050 at 3232.402 kb on - strand, within Psest_3050 at 3232.411 kb on - strand, within Psest_3050 at 3232.560 kb on - strand at 3232.689 kb on + strand, within Psest_3051 at 3232.689 kb on + strand, within Psest_3051 at 3232.713 kb on + strand, within Psest_3051 at 3232.714 kb on - strand, within Psest_3051 at 3232.742 kb on - strand, within Psest_3051 at 3232.779 kb on + strand, within Psest_3051 at 3232.779 kb on + strand, within Psest_3051 at 3232.779 kb on + strand, within Psest_3051 at 3232.780 kb on - strand, within Psest_3051 at 3232.780 kb on - strand, within Psest_3051 at 3232.780 kb on - strand, within Psest_3051 at 3232.780 kb on - strand, within Psest_3051 at 3232.792 kb on - strand, within Psest_3051 at 3232.792 kb on - strand, within Psest_3051 at 3232.835 kb on + strand, within Psest_3051 at 3232.835 kb on + strand, within Psest_3051 at 3232.835 kb on + strand, within Psest_3051 at 3232.836 kb on - strand, within Psest_3051 at 3232.836 kb on - strand, within Psest_3051 at 3232.836 kb on - strand, within Psest_3051 at 3232.836 kb on - strand, within Psest_3051 at 3232.880 kb on + strand, within Psest_3051 at 3232.880 kb on + strand, within Psest_3051 at 3232.880 kb on + strand, within Psest_3051 at 3232.881 kb on - strand, within Psest_3051 at 3232.881 kb on - strand, within Psest_3051 at 3232.881 kb on - strand, within Psest_3051 at 3232.936 kb on + strand, within Psest_3051 at 3232.937 kb on - strand, within Psest_3051 at 3232.984 kb on - strand, within Psest_3051 at 3232.985 kb on + strand, within Psest_3051 at 3232.986 kb on - strand, within Psest_3051 at 3233.059 kb on + strand, within Psest_3051 at 3233.060 kb on - strand, within Psest_3051 at 3233.107 kb on + strand, within Psest_3051 at 3233.107 kb on + strand, within Psest_3051 at 3233.108 kb on - strand, within Psest_3051 at 3233.108 kb on - strand, within Psest_3051 at 3233.108 kb on - strand, within Psest_3051 at 3233.108 kb on - strand, within Psest_3051 at 3233.137 kb on - strand, within Psest_3051 at 3233.168 kb on + strand at 3233.168 kb on + strand at 3233.168 kb on + strand at 3233.169 kb on - strand at 3233.169 kb on - strand at 3233.322 kb on - strand at 3233.548 kb on + strand, within Psest_3052 at 3233.624 kb on + strand, within Psest_3052 at 3233.625 kb on - strand, within Psest_3052 at 3233.712 kb on - strand, within Psest_3052 at 3233.794 kb on + strand, within Psest_3052 at 3233.794 kb on + strand, within Psest_3052 at 3233.871 kb on - strand, within Psest_3052 at 3233.941 kb on - strand, within Psest_3052 at 3233.987 kb on + strand, within Psest_3052 at 3233.987 kb on + strand, within Psest_3052 at 3234.085 kb on - strand, within Psest_3052 at 3234.085 kb on - strand, within Psest_3052 at 3234.339 kb on + strand at 3234.339 kb on + strand at 3234.339 kb on + strand at 3234.340 kb on - strand at 3234.340 kb on - strand at 3234.340 kb on - strand at 3234.340 kb on - strand at 3234.372 kb on + strand at 3234.372 kb on + strand at 3234.461 kb on + strand, within Psest_3053 at 3234.461 kb on + strand, within Psest_3053 at 3234.479 kb on + strand, within Psest_3053 at 3234.479 kb on + strand, within Psest_3053 at 3234.479 kb on + strand, within Psest_3053 at 3234.479 kb on + strand, within Psest_3053 at 3234.479 kb on + strand, within Psest_3053 at 3234.519 kb on + strand, within Psest_3053 at 3234.519 kb on + strand, within Psest_3053 at 3234.519 kb on + strand, within Psest_3053 at 3234.519 kb on + strand, within Psest_3053 at 3234.519 kb on + strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.520 kb on - strand, within Psest_3053 at 3234.523 kb on - strand, within Psest_3053 at 3234.531 kb on + strand, within Psest_3053 at 3234.531 kb on + strand, within Psest_3053 at 3234.532 kb on - strand, within Psest_3053 at 3234.609 kb on + strand, within Psest_3053 at 3234.768 kb on + strand, within Psest_3053 at 3234.807 kb on + strand, within Psest_3053 at 3234.807 kb on + strand, within Psest_3053 at 3234.807 kb on + strand, within Psest_3053 at 3234.807 kb on + strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.808 kb on - strand, within Psest_3053 at 3234.811 kb on + strand, within Psest_3053 at 3234.906 kb on + strand, within Psest_3053 at 3234.906 kb on + strand, within Psest_3053 at 3234.906 kb on + strand, within Psest_3053 at 3234.907 kb on - strand, within Psest_3053 at 3234.907 kb on - strand, within Psest_3053 at 3234.907 kb on - strand, within Psest_3053 at 3234.907 kb on - strand, within Psest_3053 at 3235.010 kb on - strand, within Psest_3053 at 3235.140 kb on + strand, within Psest_3053 at 3235.140 kb on + strand, within Psest_3053 at 3235.144 kb on - strand, within Psest_3053 at 3235.144 kb on - strand, within Psest_3053 at 3235.144 kb on - strand, within Psest_3053 at 3235.294 kb on - strand, within Psest_3053 at 3235.294 kb on - strand, within Psest_3053
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 3,232,399 + Psest_3050 0.14 +0.6 3,232,399 + Psest_3050 0.14 +1.1 3,232,399 + Psest_3050 0.14 +3.5 3,232,400 - Psest_3050 0.15 +0.7 3,232,400 - Psest_3050 0.15 -0.8 3,232,402 - Psest_3050 0.18 -0.5 3,232,411 - Psest_3050 0.30 +0.6 3,232,560 - -1.3 3,232,689 + Psest_3051 0.19 +1.7 3,232,689 + Psest_3051 0.19 -0.3 3,232,713 + Psest_3051 0.23 -0.9 3,232,714 - Psest_3051 0.23 +0.3 3,232,742 - Psest_3051 0.27 +2.9 3,232,779 + Psest_3051 0.33 +0.3 3,232,779 + Psest_3051 0.33 -1.1 3,232,779 + Psest_3051 0.33 -0.1 3,232,780 - Psest_3051 0.33 +1.4 3,232,780 - Psest_3051 0.33 +2.3 3,232,780 - Psest_3051 0.33 -0.7 3,232,780 - Psest_3051 0.33 +2.7 3,232,792 - Psest_3051 0.35 -2.3 3,232,792 - Psest_3051 0.35 +1.6 3,232,835 + Psest_3051 0.41 -2.1 3,232,835 + Psest_3051 0.41 +0.5 3,232,835 + Psest_3051 0.41 -1.2 3,232,836 - Psest_3051 0.41 -2.0 3,232,836 - Psest_3051 0.41 -1.1 3,232,836 - Psest_3051 0.41 +2.6 3,232,836 - Psest_3051 0.41 +0.4 3,232,880 + Psest_3051 0.48 -1.1 3,232,880 + Psest_3051 0.48 -1.1 3,232,880 + Psest_3051 0.48 -1.4 3,232,881 - Psest_3051 0.48 +1.9 3,232,881 - Psest_3051 0.48 -0.1 3,232,881 - Psest_3051 0.48 +1.5 3,232,936 + Psest_3051 0.56 -2.5 3,232,937 - Psest_3051 0.56 -1.7 3,232,984 - Psest_3051 0.63 -1.1 3,232,985 + Psest_3051 0.64 -0.3 3,232,986 - Psest_3051 0.64 -2.2 3,233,059 + Psest_3051 0.75 -1.1 3,233,060 - Psest_3051 0.75 -2.4 3,233,107 + Psest_3051 0.82 +0.3 3,233,107 + Psest_3051 0.82 +0.8 3,233,108 - Psest_3051 0.82 +2.9 3,233,108 - Psest_3051 0.82 +3.2 3,233,108 - Psest_3051 0.82 +3.7 3,233,108 - Psest_3051 0.82 -1.3 3,233,137 - Psest_3051 0.86 -2.3 3,233,168 + +0.3 3,233,168 + -0.8 3,233,168 + -0.2 3,233,169 - +0.5 3,233,169 - -1.1 3,233,322 - +3.3 3,233,548 + Psest_3052 0.25 -0.1 3,233,624 + Psest_3052 0.33 +2.1 3,233,625 - Psest_3052 0.33 +0.4 3,233,712 - Psest_3052 0.42 -2.1 3,233,794 + Psest_3052 0.50 -1.6 3,233,794 + Psest_3052 0.50 -1.9 3,233,871 - Psest_3052 0.57 -0.4 3,233,941 - Psest_3052 0.64 -3.4 3,233,987 + Psest_3052 0.69 +0.0 3,233,987 + Psest_3052 0.69 -2.3 3,234,085 - Psest_3052 0.79 -1.2 3,234,085 - Psest_3052 0.79 +0.6 3,234,339 + -0.9 3,234,339 + +0.4 3,234,339 + +0.6 3,234,340 - +0.4 3,234,340 - +2.5 3,234,340 - -1.1 3,234,340 - -1.6 3,234,372 + -1.1 3,234,372 + -1.1 3,234,461 + Psest_3053 0.14 +2.0 3,234,461 + Psest_3053 0.14 +3.3 3,234,479 + Psest_3053 0.15 -1.4 3,234,479 + Psest_3053 0.15 -0.5 3,234,479 + Psest_3053 0.15 -1.2 3,234,479 + Psest_3053 0.15 -1.8 3,234,479 + Psest_3053 0.15 +2.0 3,234,519 + Psest_3053 0.19 +0.4 3,234,519 + Psest_3053 0.19 +0.7 3,234,519 + Psest_3053 0.19 -0.6 3,234,519 + Psest_3053 0.19 +4.6 3,234,519 + Psest_3053 0.19 -1.5 3,234,520 - Psest_3053 0.19 +2.5 3,234,520 - Psest_3053 0.19 -1.1 3,234,520 - Psest_3053 0.19 -0.5 3,234,520 - Psest_3053 0.19 -2.3 3,234,520 - Psest_3053 0.19 -1.3 3,234,520 - Psest_3053 0.19 -0.1 3,234,520 - Psest_3053 0.19 -1.1 3,234,520 - Psest_3053 0.19 -1.6 3,234,520 - Psest_3053 0.19 -0.9 3,234,523 - Psest_3053 0.19 +4.8 3,234,531 + Psest_3053 0.20 -1.9 3,234,531 + Psest_3053 0.20 +0.3 3,234,532 - Psest_3053 0.20 -1.1 3,234,609 + Psest_3053 0.26 +0.2 3,234,768 + Psest_3053 0.38 +1.1 3,234,807 + Psest_3053 0.41 -1.4 3,234,807 + Psest_3053 0.41 +2.3 3,234,807 + Psest_3053 0.41 -0.9 3,234,807 + Psest_3053 0.41 -2.1 3,234,808 - Psest_3053 0.41 +0.6 3,234,808 - Psest_3053 0.41 -1.1 3,234,808 - Psest_3053 0.41 +0.1 3,234,808 - Psest_3053 0.41 +4.6 3,234,808 - Psest_3053 0.41 -2.4 3,234,808 - Psest_3053 0.41 +0.7 3,234,808 - Psest_3053 0.41 -1.4 3,234,808 - Psest_3053 0.41 +0.9 3,234,811 + Psest_3053 0.41 -1.1 3,234,906 + Psest_3053 0.49 -1.7 3,234,906 + Psest_3053 0.49 +3.3 3,234,906 + Psest_3053 0.49 +1.7 3,234,907 - Psest_3053 0.49 -1.5 3,234,907 - Psest_3053 0.49 -0.6 3,234,907 - Psest_3053 0.49 -2.0 3,234,907 - Psest_3053 0.49 -1.1 3,235,010 - Psest_3053 0.57 -0.7 3,235,140 + Psest_3053 0.67 +0.3 3,235,140 + Psest_3053 0.67 -0.5 3,235,144 - Psest_3053 0.68 -2.8 3,235,144 - Psest_3053 0.68 -1.7 3,235,144 - Psest_3053 0.68 +1.6 3,235,294 - Psest_3053 0.79 -0.3 3,235,294 - Psest_3053 0.79 -1.4
Or see this region's nucleotide sequence