Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1904 and Psest_1905 are separated by 116 nucleotides Psest_1905 and Psest_1907 are separated by 284 nucleotides Psest_1907 and Psest_1908 are separated by 3 nucleotides Psest_1908 and Psest_1909 are separated by 3 nucleotides Psest_1909 and Psest_1910 overlap by 4 nucleotides
Psest_1904: Psest_1904 - Arabinose efflux permease, at 2,014,700 to 2,015,851
_1904
Psest_1905: Psest_1905 - Uncharacterized conserved protein, contains double-stranded beta-helix domain, at 2,015,968 to 2,016,513
_1905
Psest_1907: Psest_1907 - hypothetical protein, at 2,016,798 to 2,017,007
_1907
Psest_1908: Psest_1908 - hypothetical protein, at 2,017,011 to 2,017,205
_1908
Psest_1909: Psest_1909 - Phosphohistidine phosphatase SixA, at 2,017,209 to 2,017,766
_1909
Psest_1910: Psest_1910 - 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase, at 2,017,763 to 2,018,647
_1910
Position (kb)
2016
2017
2018 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2015.816 kb on - strand at 2016.334 kb on + strand, within Psest_1905 at 2016.341 kb on + strand, within Psest_1905 at 2016.341 kb on + strand, within Psest_1905 at 2016.439 kb on - strand, within Psest_1905 at 2016.467 kb on - strand at 2016.565 kb on - strand at 2016.565 kb on - strand at 2016.565 kb on - strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.594 kb on + strand at 2016.595 kb on - strand at 2016.595 kb on - strand at 2016.595 kb on - strand at 2016.683 kb on + strand at 2016.684 kb on - strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.764 kb on + strand at 2016.765 kb on - strand at 2016.765 kb on - strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.769 kb on + strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.770 kb on - strand at 2016.773 kb on + strand at 2016.774 kb on - strand at 2016.848 kb on + strand, within Psest_1907 at 2017.129 kb on + strand, within Psest_1908 at 2017.203 kb on + strand at 2017.203 kb on + strand at 2017.312 kb on + strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.313 kb on - strand, within Psest_1909 at 2017.466 kb on + strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.467 kb on - strand, within Psest_1909 at 2017.741 kb on + strand at 2017.788 kb on + strand at 2017.789 kb on - strand at 2017.789 kb on - strand at 2017.959 kb on + strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 2,015,816 - +1.4 2,016,334 + Psest_1905 0.67 -0.7 2,016,341 + Psest_1905 0.68 -1.6 2,016,341 + Psest_1905 0.68 +0.1 2,016,439 - Psest_1905 0.86 -2.4 2,016,467 - +1.4 2,016,565 - +0.1 2,016,565 - -0.5 2,016,565 - -0.6 2,016,594 + +1.0 2,016,594 + -2.5 2,016,594 + -0.9 2,016,594 + -1.8 2,016,595 - -3.4 2,016,595 - -2.0 2,016,595 - +0.7 2,016,683 + +1.2 2,016,684 - -1.6 2,016,764 + +2.2 2,016,764 + -1.0 2,016,764 + -0.2 2,016,764 + +1.3 2,016,765 - -0.4 2,016,765 - +1.8 2,016,769 + +2.4 2,016,769 + +2.4 2,016,769 + -0.7 2,016,769 + -0.3 2,016,770 - -0.4 2,016,770 - +4.2 2,016,770 - +2.9 2,016,770 - -0.9 2,016,770 - +0.5 2,016,770 - +0.2 2,016,773 + +0.3 2,016,774 - +0.1 2,016,848 + Psest_1907 0.24 +1.1 2,017,129 + Psest_1908 0.61 +4.5 2,017,203 + +3.0 2,017,203 + -2.5 2,017,312 + Psest_1909 0.18 +1.4 2,017,313 - Psest_1909 0.19 -0.5 2,017,313 - Psest_1909 0.19 -1.6 2,017,313 - Psest_1909 0.19 +0.7 2,017,466 + Psest_1909 0.46 +0.8 2,017,467 - Psest_1909 0.46 -1.0 2,017,467 - Psest_1909 0.46 +0.5 2,017,467 - Psest_1909 0.46 +1.1 2,017,741 + +0.9 2,017,788 + +0.2 2,017,789 - -1.3 2,017,789 - -1.6 2,017,959 + Psest_1910 0.22 +1.4 2,017,960 - Psest_1910 0.22 +1.6 2,017,960 - Psest_1910 0.22 -0.2
Or see this region's nucleotide sequence