Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1885 and Psest_1886 are separated by 75 nucleotides Psest_1886 and Psest_1887 are separated by 404 nucleotides Psest_1887 and Psest_1888 are separated by 13 nucleotides Psest_1888 and Psest_1889 are separated by 46 nucleotides
Psest_1885: Psest_1885 - hypothetical protein, at 1,995,024 to 1,995,401
_1885
Psest_1886: Psest_1886 - Uncharacterized protein conserved in bacteria, at 1,995,477 to 1,995,776
_1886
Psest_1887: Psest_1887 - Predicted metal-dependent phosphoesterases (PHP family), at 1,996,181 to 1,997,047
_1887
Psest_1888: Psest_1888 - Sua5/YciO/YrdC/YwlC family protein, at 1,997,061 to 1,997,684
_1888
Psest_1889: Psest_1889 - tryptophanyl-tRNA synthetase, at 1,997,731 to 1,998,945
_1889
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1995.210 kb on + strand, within Psest_1885 at 1995.316 kb on + strand, within Psest_1885 at 1995.317 kb on - strand, within Psest_1885 at 1995.317 kb on - strand, within Psest_1885 at 1995.346 kb on - strand, within Psest_1885 at 1995.346 kb on - strand, within Psest_1885 at 1995.412 kb on - strand at 1995.420 kb on - strand at 1995.459 kb on - strand at 1995.510 kb on + strand, within Psest_1886 at 1995.511 kb on - strand, within Psest_1886 at 1995.534 kb on + strand, within Psest_1886 at 1995.535 kb on - strand, within Psest_1886 at 1995.536 kb on + strand, within Psest_1886 at 1995.537 kb on - strand, within Psest_1886 at 1995.537 kb on - strand, within Psest_1886 at 1995.537 kb on - strand, within Psest_1886 at 1995.537 kb on - strand, within Psest_1886 at 1995.538 kb on + strand, within Psest_1886 at 1995.538 kb on + strand, within Psest_1886 at 1995.538 kb on + strand, within Psest_1886 at 1995.539 kb on - strand, within Psest_1886 at 1995.539 kb on - strand, within Psest_1886 at 1995.539 kb on - strand, within Psest_1886 at 1995.769 kb on + strand at 1995.809 kb on - strand at 1995.824 kb on - strand at 1995.909 kb on - strand at 1996.108 kb on + strand at 1996.175 kb on + strand at 1996.178 kb on + strand at 1996.178 kb on + strand at 1996.178 kb on + strand at 1996.178 kb on + strand at 1996.178 kb on + strand at 1996.245 kb on + strand at 1996.381 kb on - strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.404 kb on + strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.405 kb on - strand, within Psest_1887 at 1996.897 kb on - strand, within Psest_1887 at 1997.564 kb on - strand, within Psest_1888 at 1997.642 kb on - strand at 1997.642 kb on - strand at 1997.711 kb on + strand at 1997.712 kb on - strand at 1997.712 kb on - strand at 1997.712 kb on - strand at 1997.718 kb on + strand at 1997.718 kb on + strand at 1997.719 kb on - strand at 1997.719 kb on - strand at 1997.803 kb on + strand at 1997.803 kb on + strand at 1997.932 kb on - strand, within Psest_1889 at 1997.981 kb on + strand, within Psest_1889
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,995,210 + Psest_1885 0.49 +0.5 1,995,316 + Psest_1885 0.77 +0.7 1,995,317 - Psest_1885 0.78 +3.5 1,995,317 - Psest_1885 0.78 -1.2 1,995,346 - Psest_1885 0.85 -2.8 1,995,346 - Psest_1885 0.85 +2.1 1,995,412 - -2.3 1,995,420 - -1.8 1,995,459 - -0.6 1,995,510 + Psest_1886 0.11 -1.3 1,995,511 - Psest_1886 0.11 +3.5 1,995,534 + Psest_1886 0.19 -0.7 1,995,535 - Psest_1886 0.19 +2.0 1,995,536 + Psest_1886 0.20 +1.1 1,995,537 - Psest_1886 0.20 +3.5 1,995,537 - Psest_1886 0.20 -0.8 1,995,537 - Psest_1886 0.20 -1.4 1,995,537 - Psest_1886 0.20 -2.3 1,995,538 + Psest_1886 0.20 +0.6 1,995,538 + Psest_1886 0.20 +3.8 1,995,538 + Psest_1886 0.20 -0.3 1,995,539 - Psest_1886 0.21 +0.5 1,995,539 - Psest_1886 0.21 -0.5 1,995,539 - Psest_1886 0.21 -1.3 1,995,769 + +2.1 1,995,809 - -2.7 1,995,824 - +1.5 1,995,909 - +0.8 1,996,108 + -3.3 1,996,175 + -1.2 1,996,178 + -0.9 1,996,178 + +0.4 1,996,178 + -1.9 1,996,178 + +4.3 1,996,178 + +0.3 1,996,245 + +1.0 1,996,381 - Psest_1887 0.23 -1.0 1,996,404 + Psest_1887 0.26 +0.5 1,996,404 + Psest_1887 0.26 +0.6 1,996,404 + Psest_1887 0.26 -1.6 1,996,404 + Psest_1887 0.26 -1.1 1,996,404 + Psest_1887 0.26 -0.1 1,996,404 + Psest_1887 0.26 +0.3 1,996,404 + Psest_1887 0.26 +0.3 1,996,405 - Psest_1887 0.26 -1.7 1,996,405 - Psest_1887 0.26 -0.2 1,996,405 - Psest_1887 0.26 -0.1 1,996,405 - Psest_1887 0.26 +1.2 1,996,405 - Psest_1887 0.26 -0.4 1,996,405 - Psest_1887 0.26 +1.5 1,996,897 - Psest_1887 0.83 -0.7 1,997,564 - Psest_1888 0.81 -3.5 1,997,642 - +2.0 1,997,642 - +3.5 1,997,711 + +2.0 1,997,712 - +0.6 1,997,712 - +0.5 1,997,712 - +0.1 1,997,718 + -0.9 1,997,718 + +3.3 1,997,719 - -2.1 1,997,719 - +1.5 1,997,803 + -2.8 1,997,803 + +0.0 1,997,932 - Psest_1889 0.17 +1.4 1,997,981 + Psest_1889 0.21 -2.4
Or see this region's nucleotide sequence