Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1800 and Psest_1801 are separated by 199 nucleotides Psest_1801 and Psest_1802 are separated by 132 nucleotides
Psest_1800: Psest_1800 - anion transporter, at 1,894,776 to 1,896,224
_1800
Psest_1801: Psest_1801 - 3-deoxy-7-phosphoheptulonate synthase, class II, at 1,896,424 to 1,897,773
_1801
Psest_1802: Psest_1802 - Spermidine synthase, at 1,897,906 to 1,898,655
_1802
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1895.517 kb on + strand, within Psest_1800 at 1895.518 kb on - strand, within Psest_1800 at 1895.584 kb on + strand, within Psest_1800 at 1895.584 kb on + strand, within Psest_1800 at 1895.584 kb on + strand, within Psest_1800 at 1895.585 kb on - strand, within Psest_1800 at 1895.585 kb on - strand, within Psest_1800 at 1895.786 kb on + strand, within Psest_1800 at 1895.787 kb on - strand, within Psest_1800 at 1896.238 kb on + strand at 1896.238 kb on + strand at 1896.238 kb on + strand at 1896.239 kb on - strand at 1896.243 kb on - strand at 1896.243 kb on - strand at 1896.243 kb on - strand at 1896.243 kb on - strand at 1896.243 kb on - strand at 1896.298 kb on + strand at 1896.299 kb on - strand at 1896.299 kb on - strand at 1896.308 kb on + strand at 1896.312 kb on + strand at 1896.312 kb on + strand at 1896.340 kb on + strand at 1896.340 kb on + strand at 1896.341 kb on - strand at 1896.341 kb on - strand at 1896.341 kb on - strand at 1896.343 kb on + strand at 1896.343 kb on + strand at 1896.351 kb on + strand at 1896.351 kb on + strand at 1896.351 kb on + strand at 1896.352 kb on - strand at 1896.390 kb on + strand at 1896.390 kb on + strand at 1896.390 kb on + strand at 1896.390 kb on + strand at 1896.412 kb on + strand at 1896.412 kb on + strand at 1896.412 kb on + strand at 1896.413 kb on - strand at 1896.413 kb on - strand at 1896.518 kb on + strand at 1896.615 kb on - strand, within Psest_1801 at 1896.690 kb on - strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.056 kb on - strand, within Psest_1801 at 1897.056 kb on - strand, within Psest_1801 at 1897.143 kb on + strand, within Psest_1801 at 1897.178 kb on + strand, within Psest_1801 at 1897.178 kb on + strand, within Psest_1801 at 1897.855 kb on + strand at 1897.856 kb on - strand at 1897.965 kb on - strand at 1898.024 kb on + strand, within Psest_1802 at 1898.025 kb on - strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.121 kb on - strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.653 kb on + strand at 1898.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,895,517 + Psest_1800 0.51 +0.6 1,895,518 - Psest_1800 0.51 +1.0 1,895,584 + Psest_1800 0.56 +0.2 1,895,584 + Psest_1800 0.56 -0.9 1,895,584 + Psest_1800 0.56 +5.4 1,895,585 - Psest_1800 0.56 -1.1 1,895,585 - Psest_1800 0.56 +3.1 1,895,786 + Psest_1800 0.70 +2.6 1,895,787 - Psest_1800 0.70 -1.6 1,896,238 + -2.7 1,896,238 + +0.4 1,896,238 + +0.2 1,896,239 - -0.8 1,896,243 - -0.7 1,896,243 - +1.5 1,896,243 - -3.5 1,896,243 - -1.9 1,896,243 - +0.6 1,896,298 + +1.1 1,896,299 - +0.4 1,896,299 - -1.2 1,896,308 + -1.0 1,896,312 + -0.1 1,896,312 + -0.7 1,896,340 + +2.2 1,896,340 + -1.8 1,896,341 - -0.5 1,896,341 - -1.9 1,896,341 - +0.1 1,896,343 + +2.6 1,896,343 + +3.9 1,896,351 + +0.2 1,896,351 + -1.2 1,896,351 + +3.2 1,896,352 - -1.5 1,896,390 + +0.5 1,896,390 + -0.9 1,896,390 + +0.6 1,896,390 + +1.7 1,896,412 + -0.5 1,896,412 + -2.8 1,896,412 + +0.3 1,896,413 - +0.5 1,896,413 - -3.3 1,896,518 + +2.6 1,896,615 - Psest_1801 0.14 +0.5 1,896,690 - Psest_1801 0.20 -0.2 1,897,055 + Psest_1801 0.47 -1.1 1,897,055 + Psest_1801 0.47 +2.0 1,897,055 + Psest_1801 0.47 +0.9 1,897,055 + Psest_1801 0.47 +2.3 1,897,055 + Psest_1801 0.47 -1.5 1,897,055 + Psest_1801 0.47 +1.7 1,897,056 - Psest_1801 0.47 -0.2 1,897,056 - Psest_1801 0.47 -0.5 1,897,143 + Psest_1801 0.53 +2.9 1,897,178 + Psest_1801 0.56 +2.6 1,897,178 + Psest_1801 0.56 +0.4 1,897,855 + -1.0 1,897,856 - +3.7 1,897,965 - +0.6 1,898,024 + Psest_1802 0.16 -2.5 1,898,025 - Psest_1802 0.16 -1.9 1,898,059 + Psest_1802 0.20 +0.2 1,898,059 + Psest_1802 0.20 -2.6 1,898,121 - Psest_1802 0.29 -2.8 1,898,257 + Psest_1802 0.47 -2.9 1,898,257 + Psest_1802 0.47 +0.3 1,898,257 + Psest_1802 0.47 +1.3 1,898,257 + Psest_1802 0.47 +1.9 1,898,257 + Psest_1802 0.47 -1.7 1,898,258 - Psest_1802 0.47 +1.4 1,898,258 - Psest_1802 0.47 +4.1 1,898,258 - Psest_1802 0.47 -1.5 1,898,258 - Psest_1802 0.47 -0.8 1,898,258 - Psest_1802 0.47 +2.0 1,898,330 - Psest_1802 0.57 -0.2 1,898,330 - Psest_1802 0.57 -1.3 1,898,653 + +1.0 1,898,654 - +1.3
Or see this region's nucleotide sequence